Key features and details
- P2 purinergic receptor antagonist
- CAS Number: 149017-66-3
- Soluble in water to 100 mM
- Form / State: Solid
- Source: Synthetic
Product namePPADS, P2 purinergic receptor antagonist
DescriptionP2 purinergic receptor antagonist
P2 purinergic receptor antagonist.
Chemical name4-[[4-Formyl-5-hydroxy-6-methyl-3-[(phosphonooxy)methyl]-2-pyridinyl]azo]-1,3-benzenedisulfonic acid tetrasodium salt
Storage instructionsStore at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months.
Solubility overviewSoluble in water to 100 mM
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one week. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
2D chemical structure image of ab120009, PPADS, P2 purinergic receptor antagonist
Inhibition by suramin and PPADS significantly reduces the ATP-induced increase in extracellular H+ flux from isolated Müller cells.
Mean responses to 10 µM ATP with or without suramin and PPADS in the bath; N = 7, error bars represent SEMs.
Astrocyte Ca2+ signal inhibition does not affect interictal discharges. (A–D) Mean percentage of astrocytes activated by the ictal discharges (A), mean duration (B) and frequency (C) of the ictal discharge, and mean frequency of interictal discharges (D) under different experimental conditions in EC slice preparations. Controls (n=16), MPEP (ab120008) (n=7), PPADS (ab120009) (n=9), and MPEP+PPADS (n=3). A single asterisk (*) indicates p<0.05; double asterisks (**), p<0.01.
Ca2+ signal from a field A neuron, a field B neuron, and field A astrocytes in response to repetitive episodes of NMDA stimulation (black arrowheads). The NMDA stimulation that evoked an ictal discharge became ineffective after blocking the astrocyte response by bath perfusion with MPEP (ab120008) and PPADS (ab120009). An ictal discharge could be recovered by increasing the number of NMDA puffs (white arrowheads). A double NMDA pulse evoked both astrocyte activation and the ictal discharge after inhibitor washout.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab120009 has been referenced in 9 publications.
- Pulukool SK et al. Elevated dimethylarginine, ATP, cytokines, metabolic remodeling involving tryptophan metabolism and potential microglial inflammation characterize primary open angle glaucoma. Sci Rep 11:9766 (2021). PubMed: 33963197
- Bhagavatham SKS et al. Adenosine deaminase modulates metabolic remodeling and orchestrates joint destruction in rheumatoid arthritis. Sci Rep 11:15129 (2021). PubMed: 34301999
- Su WF et al. Overexpression of P2X4 receptor in Schwann cells promotes motor and sensory functional recovery and remyelination via BDNF secretion after nerve injury. Glia 67:78-90 (2019). PubMed: 30306657
- Tchernookova BK et al. Activation of retinal glial (Müller) cells by extracellular ATP induces pronounced increases in extracellular H+ flux. PLoS One 13:e0190893 (2018). PubMed: 29466379
- Ferreira-Neto HC et al. ATP stimulates rat hypothalamic sympathetic neurons by enhancing AMPA receptor-mediated currents. J Neurophysiol 114:159-69 (2015). PubMed: 25904713
- Heo SJ et al. Biophysical Regulation of Chromatin Architecture Instills a Mechanical Memory in Mesenchymal Stem Cells. Sci Rep 5:16895 (2015). PubMed: 26592929
- Kovacs FE et al. Purine receptor-mediated endocannabinoid production and retrograde synaptic signalling in the cerebellar cortex. Br J Pharmacol 162:974-88 (2011). PubMed: 21054344
- Toulme E et al. P2X4 receptors in activated C8-B4 cells of cerebellar microglial origin. J Gen Physiol 135:333-53 (2010). PubMed: 20231374
- Gómez-Gonzalo M et al. An excitatory loop with astrocytes contributes to drive neurons to seizure threshold. PLoS Biol 8:e1000352 (2010). PubMed: 20405049