Key features and details
- Mouse monoclonal [OTI1H4] to PPAP2A
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-PPAP2A antibody [OTI1H4]
See all PPAP2A primary antibodies
DescriptionMouse monoclonal [OTI1H4] to PPAP2A
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Guinea pig
Recombinant full length protein corresponding to Human PPAP2A aa 1-284.
MFDKTRLPYVALDVLCVLLAGLPFAILTSRHTPFQRGVFCNDESIKYPYK EDTIPYALLGGIIIPFSIIVIILGETLSVYCNLLHSNSFIRNNYIATIYK AIGTFLFGAAASQSLTDIAKYSIGRLRPHFLDVCDPDWSKINCSDGYIEY YICRGNAERVKEGRLSFYSGHSSFSMYCMLFVALYLQARMKGDWARLLRP TLQFGLVAVSIYVGLSRVSDYKHHWSDVLTGLIQGALVAILVAVYVSDFF KERTSFKERKEEDSHTTLHETPTTGNHYPSNHQP
Database link: NP_003702
- WB: HEK-293T cell lysate transfected with pCMV6-ENTRY PPAP2A cDNA. IHC-P: Human kidney, kidney carcinoma, liver, endometrium adenocarcinoma and lymph node tissues.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 1% BSA
Concentration information loading...
Purification notesPurified from cell culture supernatant by affinity chromatography
Our Abpromise guarantee covers the use of ab170151 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500. Predicted molecular weight: 32 kDa.|
FunctionBroad-specificity phosphohydrolase that dephosphorylates exogenous bioactive glycerolipids and sphingolipids. Catalyzes the conversion of phosphatidic acid (PA) to diacylglycerol (DG). Pivotal regulator of lysophosphatidic acid (LPA) signaling in the cardiovascular system. Major enzyme responsible of dephosphorylating LPA in platelets, which terminates signaling actions of LPA. May control circulating, and possibly also regulate localized, LPA levels resulting from platelet activation. It has little activity towards ceramide-1-phosphate (C-1-P) and sphingosine-1-phosphate (S-1-P). The relative catalytic efficiency is LPA > PA > S-1-P > C-1-P. It's down-regulation may contribute to the development of colon adenocarcinoma.
Tissue specificityUbiquitously expressed with highest expression found in prostate. Isoform 1 is predominant in kidney, lung, placenta and liver. Isoform 2 is predominant in heart and pancreas. Found to be down-regulated in colon adenocarcinomas.
Sequence similaritiesBelongs to the PA-phosphatase related phosphoesterase family.
modificationsN-glycosylated. Contains high-mannose oligosaccharides.
Cellular localizationCell membrane.
- Information by UniProt
- Lipid phosphate phosphohydrolase 1 antibody
- Lipid phosphate phosphohydrolase 1a antibody
- LLP1a antibody
Paraffin-embedded human lymph node tissue stained for PPAP2A using ab170151 at 1/150 dilution in immunohistochemical analysis.
Paraffin-embedded human endometrium adenocarcinoma tissue stained for PPAP2A using ab170151 at 1/150 dilution in immunohistochemical analysis.
Paraffin-embedded human liver tissue stained for PPAP2A using ab170151 at 1/150 dilution in immunohistochemical analysis.
Paraffin-embedded human kidney carcinoma tissue stained for PPAP2A using ab170151 at 1/150 dilution in immunohistochemical analysis.
Paraffin-embedded human kidney tissue stained for PPAP2A using ab170151 at 1/150 dilution in immunohistochemical analysis.
All lanes : Anti-PPAP2A antibody [OTI1H4] (ab170151) at 1/500 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate transfected with pCMV6-ENTRY control
Lane 2 : HEK-293T cell lysate transfected with pCMV6-ENTRY PPAP2A cDNA
Lysates/proteins at 5 µg per lane.
Predicted band size: 32 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab170151 has not yet been referenced specifically in any publications.