Anti-PPAP2C antibody (ab236857)
Key features and details
- Rabbit polyclonal to PPAP2C
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PPAP2C antibody
See all PPAP2C primary antibodies -
Description
Rabbit polyclonal to PPAP2C -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human PPAP2C aa 109-162.
Database link: O43688 -
Positive control
- WB: A549 and MCF7 whole cell lysates. IHC-P: Human colon cancer and small intestine tissues. ICC/IF: A549 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300 -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purity >95%. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab236857 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/500 - 1/2000. Predicted molecular weight: 33 kDa.
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| IHC-P |
1/20 - 1/200.
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| ICC/IF |
1/50 - 1/200.
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| Notes |
|---|
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WB
1/500 - 1/2000. Predicted molecular weight: 33 kDa. |
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IHC-P
1/20 - 1/200. |
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ICC/IF
1/50 - 1/200. |
Target
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Relevance
PPAP2C belongs to the PA-phosphatase related phosphoesterase family. It catalyzes the conversion of phosphatidic acid (PA) to diacylglycerol (DG). In addition it hydrolyzes lysophosphatidic acid (LPA), ceramide-1-phosphate (C-1-P) and sphingosine-1-phosphate (S-1-P). The relative catalytic efficiency is PA > C-1-P > LPA > S-1-P. PPAP2C is found mainly in brain, pancreas and placenta. -
Cellular localization
Membrane; Multi-pass membrane protein. -
Database links
- Entrez Gene: 8612 Human
- Omim: 607126 Human
- SwissProt: O43688 Human
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Alternative names
- EC 3.1.3.4 antibody
- Lipid phosphate phosphohydrolase 2 antibody
- LPP2 antibody
see all
Images
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Western blot - Anti-PPAP2C antibody (ab236857)All lanes : Anti-PPAP2C antibody (ab236857) at 1/500 dilution
Lane 1 : A549 (human lung carcinoma cell line) whole cell lysate
Lane 2 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Secondary
All lanes : Goat polyclonal to Rabbit IgG at 1/10000 dilution
Predicted band size: 33 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPAP2C antibody (ab236857)Paraffin-embedded human colon cancer tissue stained for PPAP2C using ab236857 at 1/100 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPAP2C antibody (ab236857)Paraffin-embedded human small intestine tissue stained for PPAP2C using ab236857 at 1/100 dilution in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-PPAP2C antibody (ab236857)A549 (human lung carcinoma cell line) cells stained for PPAP2C (green) using ab236857 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488-congugated Goat Anti-Rabbit IgG (H+L) secondary antibody.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab236857 has not yet been referenced specifically in any publications.