Anti-PPAP2C antibody (ab236857)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-PPAP2C antibody
See all PPAP2C primary antibodies -
Description
Rabbit polyclonal to PPAP2C -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human PPAP2C aa 109-162.
Database link: O43688 -
Positive control
- WB: A549 and MCF7 whole cell lysates. IHC-P: Human colon cancer and small intestine tissues. ICC/IF: A549 cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.03% Proclin
Constituents: 50% Glycerol, PBS -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purity >95%. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Related Products
Applications
Our Abpromise guarantee covers the use of ab236857 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/2000. Predicted molecular weight: 33 kDa. | |
| IHC-P | 1/20 - 1/200. | |
| ICC/IF | 1/50 - 1/200. |
Target
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Relevance
PPAP2C belongs to the PA-phosphatase related phosphoesterase family. It catalyzes the conversion of phosphatidic acid (PA) to diacylglycerol (DG). In addition it hydrolyzes lysophosphatidic acid (LPA), ceramide-1-phosphate (C-1-P) and sphingosine-1-phosphate (S-1-P). The relative catalytic efficiency is PA > C-1-P > LPA > S-1-P. PPAP2C is found mainly in brain, pancreas and placenta. -
Cellular localization
Membrane; Multi-pass membrane protein. -
Database links
- Entrez Gene: 8612 Human
- Omim: 607126 Human
- SwissProt: O43688 Human
- Unigene: 465506 Human
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Alternative names
- EC 3.1.3.4 antibody
- Lipid phosphate phosphohydrolase 2 antibody
- LPP2 antibody
see all
Images
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Western blot - Anti-PPAP2C antibody (ab236857)All lanes : Anti-PPAP2C antibody (ab236857) at 1/500 dilution
Lane 1 : A549 (human lung carcinoma cell line) whole cell lysate
Lane 2 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Secondary
All lanes : Goat polyclonal to Rabbit IgG at 1/10000 dilution
Predicted band size: 33 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPAP2C antibody (ab236857)Paraffin-embedded human colon cancer tissue stained for PPAP2C using ab236857 at 1/100 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPAP2C antibody (ab236857)Paraffin-embedded human small intestine tissue stained for PPAP2C using ab236857 at 1/100 dilution in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-PPAP2C antibody (ab236857)A549 (human lung carcinoma cell line) cells stained for PPAP2C (green) using ab236857 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488-congugated Goat Anti-Rabbit IgG (H+L) secondary antibody.
References
ab236857 has not yet been referenced specifically in any publications.
