Overview

  • Product name

    Anti-PPAPDC1A antibody
  • Description

    Rabbit polyclonal to PPAPDC1A
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Chinese hamster, Orangutan
  • Immunogen

    Recombinant fragment, corresponding to amino acids 223-271 of Human PPAPDC1A (Q5VZY2).

  • Positive control

    • RT-4, U-251 MG and Human Liver cell lysates, Human cerebellum tissue
  • General notes

    The antibody solution should be gently mixed before use.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 50% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG

Applications

Our Abpromise guarantee covers the use of ab150925 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/250 - 1/500. Predicted molecular weight: 30 kDa.
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

Target

  • Function

    Displays magnesium-independent phosphatidate phosphatase activity in vitro. Catalyzes the conversion of phosphatidic acid to diacylglycerol.
  • Tissue specificity

    Expressed mainly to the brain, kidney and testis, and to a lesser extent the bone marrow, thymus, prostate, liver and uterus.
  • Sequence similarities

    Belongs to the PA-phosphatase related phosphoesterase family.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • DPPL2 antibody
    • EC=3.1.3.4 antibody
    • Phosphatidate phosphatase PPAPDC1A antibody
    • Phosphatidic acid phosphatase type 2 domain-containing protein 1A antibody
    • PPAPDC1 antibody
    • PPAPDC1A antibody
    • PPC1A_HUMAN antibody
    see all

Images

  • Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleoli. Recommended concentration of ab150925 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • All lanes : Anti-PPAPDC1A antibody (ab150925) at 1/250 dilution

    Lane 1 : RT-4 cell lysate
    Lane 2 : U-251 MG cell lysate
    Lane 3 : Human Plasma cell lysate
    Lane 4 : Human Liver cell lysate
    Lane 5 : Human Tonsil cell lysate

    Predicted band size: 30 kDa

  • Immunohistochemical analysis of paraffin embedded Human cerebellum tissue labeling PPAPDC1A with ab150925 antibody at 1/200.

References

This product has been referenced in:

  • Zhang X  et al. Phospholipid Phosphatase 4 promotes proliferation and tumorigenesis, and activates Ca2+-permeable Cationic Channel in lung carcinoma cells. Mol Cancer 16:147 (2017). Read more (PubMed: 28851360) »
See 1 Publication for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab150925.
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