Anti-PPAR alpha antibody (ab126285)
Key features and details
- Rabbit polyclonal to PPAR alpha
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-PPAR alpha antibody
See all PPAR alpha primary antibodies -
Description
Rabbit polyclonal to PPAR alpha -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Cow, Dog, Macaque monkey, Gorilla, Orangutan
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Immunogen
Synthetic peptide corresponding to Human PPAR alpha aa 450 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab140319) -
Positive control
- This antibody gave a positive signal in the following whole cell lysates: HepG2; HEK293; NIH3T3; Jurkat.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Nucleotide metabolism
- Molecular processes
- Mitochondrial transcription
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab126285 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
Use a concentration of 1 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).
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| Notes |
|---|
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa). |
Target
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Function
Ligand-activated transcription factor. Key regulator of lipid metabolism. Activated by the endogenous ligand 1-palmitoyl-2-oleoyl-sn-glycerol-3-phosphocholine (16:0/18:1-GPC). Activated by oleylethanolamide, a naturally occurring lipid that regulates satiety (By similarity). Receptor for peroxisome proliferators such as hypolipidemic drugs and fatty acids. Regulates the peroxisomal beta-oxidation pathway of fatty acids. Functions as transcription activator for the ACOX1 and P450 genes. Transactivation activity requires heterodimerization with RXRA and is antagonized by NR2C2. -
Tissue specificity
Skeletal muscle, liver, heart and kidney. -
Sequence similarities
Belongs to the nuclear hormone receptor family. NR1 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 281992 Cow
- Entrez Gene: 403654 Dog
- Entrez Gene: 5465 Human
- Entrez Gene: 19013 Mouse
- Entrez Gene: 25747 Rat
- Omim: 170998 Human
- SwissProt: Q95N78 Dog
- SwissProt: Q07869 Human
see all -
Alternative names
- hPPAR antibody
- MGC2237 antibody
- MGC2452 antibody
see all
Images
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Western blot - Anti-PPAR alpha antibody (ab126285)All lanes : Anti-PPAR alpha antibody (ab126285) at 1 µg/ml
Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 4 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 52 kDa
Observed band size: 52 kDa
Additional bands at: 37 kDa, 50 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 1 minute
Datasheets and documents
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SDS download
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Datasheet download
References (8)
ab126285 has been referenced in 8 publications.
- Shao Z et al. RNA Sequence Analyses throughout the Course of Mouse Cardiac Laminopathy Identify Differentially Expressed Genes for Cell Cycle Control and Mitochondrial Function. Sci Rep 10:6632 (2020). PubMed: 32313136
- Jestin M et al. Mitochondrial disease disrupts hepatic allostasis and lowers the threshold for immune-mediated liver toxicity. Mol Metab 37:100981 (2020). PubMed: 32283081
- Matlock HG et al. Pathogenic Role of PPARa Downregulation in Corneal Nerve Degeneration and Impaired Corneal Sensitivity in Diabetes. Diabetes 69:1279-1291 (2020). PubMed: 32213513
- Zhu XY et al. In vitro and in vivo approaches for identifying the role of aryl hydrocarbon receptor in the development of nonalcoholic fatty liver disease. Toxicol Lett 319:85-94 (2020). PubMed: 31730885
- Wang J et al. Inhibited Lipophagy Suppresses Lipid Metabolism in Zebrafish Liver Cells. Front Physiol 10:1077 (2019). PubMed: 31496957
- Gu L et al. Spexin alleviates insulin resistance and inhibits hepatic gluconeogenesis via the FoxO1/PGC-1a pathway in high-fat-diet-induced rats and insulin resistant cells. Int J Biol Sci 15:2815-2829 (2019). PubMed: 31853220
- Guillory B et al. Ghrelin deletion protects against age-associated hepatic steatosis by downregulating the C/EBPa-p300/DGAT1 pathway. Aging Cell 17:N/A (2018). WB ; Mouse . PubMed: 29024407
- Zhu Y et al. Expression patterns of hepatic genes involved in lipid metabolism in cows with subclinical or clinical ketosis. J Dairy Sci N/A:N/A (2018). PubMed: 30471902