For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

We use cookies to make our site as useful as possible.

Our Cookie Policy explains how you can opt-out of the cookies we use.

If you continue without changing your cookie settings, we'll assume you’re happy with this.

Continue Continue

United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Diagnostic & Therapeutic Solutions
    Custom solutions & partnerships

    Custom antibody development and commercial partnerships to advance your diagnostic and therapeutic discovery.

    Create custom solutions with us

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

Supporting our customers and employees during the COVID-19 pandemic. Read more

  1. Link

    ppar-alpha-antibody-ab126285.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Epigenetics and Nuclear Signaling Transcription Domain Families Zinc Finger
Share by email

Anti-PPAR alpha antibody (ab126285)

  • Datasheet
  • SDS
Reviews (1)Q&A (1)References (8)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Western blot - Anti-PPAR alpha antibody (ab126285)

    Key features and details

    • Rabbit polyclonal to PPAR alpha
    • Suitable for: WB
    • Reacts with: Mouse, Human
    • Isotype: IgG

    You may also be interested in

    Primary
    Product image
    Anti-CD64 antibody (ab203349)
    Secondary
    Product image
    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    Primary
    Product image
    Anti-CD86 antibody [BU63] - BSA and Azide free (ab213044)

    View more associated products

    Overview

    • Product name

      Anti-PPAR alpha antibody
      See all PPAR alpha primary antibodies
    • Description

      Rabbit polyclonal to PPAR alpha
    • Host species

      Rabbit
    • Tested applications

      Suitable for: WBmore details
    • Species reactivity

      Reacts with: Mouse, Human
      Predicted to work with: Rat, Rabbit, Cow, Dog, Macaque monkey, Gorilla, Orangutan
    • Immunogen

      Synthetic peptide corresponding to Human PPAR alpha aa 450 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
      (Peptide available as ab140319)

    • Positive control

      • This antibody gave a positive signal in the following whole cell lysates: HepG2; HEK293; NIH3T3; Jurkat.
    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • Storage buffer

      pH: 7.40
      Preservative: 0.02% Sodium azide
      Constituent: PBS

      Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Epigenetics and Nuclear Signaling
      • Transcription
      • Domain Families
      • Zinc Finger
      • Cancer
      • Signal transduction
      • Nuclear signaling
      • Nuclear hormone receptors
      • Other
      • Cardiovascular
      • Lipids / Lipoproteins
      • Fatty Acids
      • Metabolism
      • Cancer
      • Cancer Metabolism
      • Metabolic signaling pathway
      • Metabolism of lipids and lipoproteins
      • Cancer
      • Cancer Metabolism
      • Response to hypoxia
      • Metabolism
      • Pathways and Processes
      • Mitochondrial Metabolism
      • Mitochondrial Biogenesis
      • Metabolism
      • Pathways and Processes
      • Metabolic signaling pathways
      • Nucleotide metabolism
      • Molecular processes
      • Mitochondrial transcription
      • Metabolism
      • Pathways and Processes
      • Metabolic signaling pathways
      • Lipid and lipoprotein metabolism
      • Lipid metabolism
      • Metabolism
      • Pathways and Processes
      • Metabolic signaling pathways
      • Lipid and lipoprotein metabolism
      • Fatty acids
      • Metabolism
      • Pathways and Processes
      • Metabolism processes
      • Hypoxia
      • Metabolism
      • Types of disease
      • Obesity
      • Metabolism
      • Pathways and Processes
      • Redox metabolism
      • Fatty acid oxidation

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Recombinant Protein

      • Recombinant Human PPAR alpha protein (ab81927)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab126285 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB (1)
    Use a concentration of 1 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).
    Notes
    WB
    Use a concentration of 1 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).

    Target

    • Function

      Ligand-activated transcription factor. Key regulator of lipid metabolism. Activated by the endogenous ligand 1-palmitoyl-2-oleoyl-sn-glycerol-3-phosphocholine (16:0/18:1-GPC). Activated by oleylethanolamide, a naturally occurring lipid that regulates satiety (By similarity). Receptor for peroxisome proliferators such as hypolipidemic drugs and fatty acids. Regulates the peroxisomal beta-oxidation pathway of fatty acids. Functions as transcription activator for the ACOX1 and P450 genes. Transactivation activity requires heterodimerization with RXRA and is antagonized by NR2C2.
    • Tissue specificity

      Skeletal muscle, liver, heart and kidney.
    • Sequence similarities

      Belongs to the nuclear hormone receptor family. NR1 subfamily.
      Contains 1 nuclear receptor DNA-binding domain.
    • Cellular localization

      Nucleus.
    • Target information above from: UniProt accession Q07869 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 281992 Cow
      • Entrez Gene: 403654 Dog
      • Entrez Gene: 5465 Human
      • Entrez Gene: 19013 Mouse
      • Entrez Gene: 25747 Rat
      • Omim: 170998 Human
      • SwissProt: Q95N78 Dog
      • SwissProt: Q07869 Human
      • SwissProt: P23204 Mouse
      • SwissProt: P37230 Rat
      • Unigene: 103110 Human
      • Unigene: 710044 Human
      • Unigene: 212789 Mouse
      • Unigene: 9753 Rat
      see all
    • Alternative names

      • hPPAR antibody
      • MGC2237 antibody
      • MGC2452 antibody
      • NR1C1 antibody
      • Nuclear receptor subfamily 1 group C member 1 antibody
      • OTTHUMP00000197740 antibody
      • OTTHUMP00000197741 antibody
      • Peroxisome proliferative activated receptor alpha antibody
      • Peroxisome proliferator activated receptor alpha antibody
      • Peroxisome proliferator-activated receptor alpha antibody
      • PPAR antibody
      • PPAR-alpha antibody
      • ppara antibody
      • PPARA_HUMAN antibody
      • PPARalpha antibody
      see all

    Images

    • Western blot - Anti-PPAR alpha antibody (ab126285)
      Western blot - Anti-PPAR alpha antibody (ab126285)
      All lanes : Anti-PPAR alpha antibody (ab126285) at 1 µg/ml

      Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
      Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
      Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
      Lane 4 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 52 kDa
      Observed band size: 52 kDa
      Additional bands at: 37 kDa, 50 kDa. We are unsure as to the identity of these extra bands.


      Exposure time: 1 minute

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (8)

    Publishing research using ab126285? Please let us know so that we can cite the reference in this datasheet.

    ab126285 has been referenced in 8 publications.

    • Shao Z  et al. RNA Sequence Analyses throughout the Course of Mouse Cardiac Laminopathy Identify Differentially Expressed Genes for Cell Cycle Control and Mitochondrial Function. Sci Rep 10:6632 (2020). PubMed: 32313136
    • Jestin M  et al. Mitochondrial disease disrupts hepatic allostasis and lowers the threshold for immune-mediated liver toxicity. Mol Metab 37:100981 (2020). PubMed: 32283081
    • Matlock HG  et al. Pathogenic Role of PPARa Downregulation in Corneal Nerve Degeneration and Impaired Corneal Sensitivity in Diabetes. Diabetes 69:1279-1291 (2020). PubMed: 32213513
    • Zhu XY  et al. In vitro and in vivo approaches for identifying the role of aryl hydrocarbon receptor in the development of nonalcoholic fatty liver disease. Toxicol Lett 319:85-94 (2020). PubMed: 31730885
    • Wang J  et al. Inhibited Lipophagy Suppresses Lipid Metabolism in Zebrafish Liver Cells. Front Physiol 10:1077 (2019). PubMed: 31496957
    • Gu L  et al. Spexin alleviates insulin resistance and inhibits hepatic gluconeogenesis via the FoxO1/PGC-1a pathway in high-fat-diet-induced rats and insulin resistant cells. Int J Biol Sci 15:2815-2829 (2019). PubMed: 31853220
    • Guillory B  et al. Ghrelin deletion protects against age-associated hepatic steatosis by downregulating the C/EBPa-p300/DGAT1 pathway. Aging Cell 17:N/A (2018). WB ; Mouse . PubMed: 29024407
    • Zhu Y  et al. Expression patterns of hepatic genes involved in lipid metabolism in cows with subclinical or clinical ketosis. J Dairy Sci N/A:N/A (2018). PubMed: 30471902

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-2 of 2 Abreviews or Q&A

    Western blot abreview for Anti-PPAR alpha antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    40 µg
    Gel Running Conditions
    Reduced Denaturing (7.5)
    Sample
    Rabbit Tissue lysate - whole (liver)
    Specification
    liver
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 7.5% · Temperature: 22°C
    Read More

    Abcam user community

    Verified customer

    Submitted Jun 25 2013

    Question

    Hola :
    Muchas gracias por tu respuesta. Aun no tenemos claro cuál es la tiolasa que necesitamos, pero estamos muy interesados en el anticuerpo para PPAR-alpha, por favor en cuanto tengas noticias me cuentas.
    Tenía una consulta acerca de los abpoints ¿son canjeables por bonos de compra en amazon o para descuentos de compra? Muchas gracias por todo.
    Un saludo

    Read More

    Abcam community

    Verified customer

    Asked on Jan 10 2013

    Answer

    Perdona por la espera.

    He estado consultando las posibilidades de reaccionar con lisados celulares de conejos entre los anticuerpos contra PPAR alpha que habíamos comentado.

    Tengo buenas noticias, pues varios de ellos muestran porcentajes altos de homología con la proteína en conejo. De todos ellos, quizás el que más posibilidades tiene de funcionar en las condiciones que buscáis sea ab126285.

    Si estas interesada en comprar el anticuerpo y participar en el sistema Abreview de evaluación de anticuerpos, no dudes en comentármelo para que primero te envíe el código canjeable por el importe del anticuerpo.

    Espero entonces tu respuesta. Que pases una buena tarde.

    Read More

    Abcam Scientific Support

    Answered on Jan 10 2013

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Get resources and offers direct to your inbox Sign up
    A-Z by research area
    • Cancer
    • Cardiovascular
    • Cell biology
    • Developmental biology
    • Epigenetics & Nuclear signaling
    • Immunology
    • Metabolism
    • Microbiology
    • Neuroscience
    • Signal transduction
    • Stem cells
    A-Z by product type
    • Primary antibodies
    • Secondary antibodies
    • Biochemicals
    • Isotype controls
    • Flow cytometry multi-color selector
    • Kits
    • Loading controls
    • Lysates
    • Peptides
    • Proteins
    • Slides
    • Tags and cell markers
    • Tools & Reagents
    Help & support
    • Support
    • Make an Inquiry
    • Protocols & troubleshooting
    • Placing an order
    • RabMAb products
    • Biochemical product FAQs
    • Training
    • Browse by Target
    Company
    • Corporate site
    • Investor relations
    • Company news
    • Careers
    • About us
    • Blog
    Events
    • Tradeshows
    • Conferences
    International websites
    • abcam.cn
    • abcam.co.jp

    Join with us

    • LinkedIn
    • facebook
    • Twitter
    • YouTube
    • Terms of sale
    • Website terms of use
    • Cookie policy
    • Privacy policy
    • Legal
    • Modern slavery statement
    © 1998-2021 Abcam plc. All rights reserved.