Anti-PPAR alpha antibody (ab126285)
- Datasheet
- References (2)
- Protocols
Overview
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Product name
Anti-PPAR alpha antibody
See all PPAR alpha primary antibodies -
Description
Rabbit polyclonal to PPAR alpha -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rabbit, Human
Predicted to work with: Rat, Cow, Dog, Macaque monkey, Gorilla, Orangutan
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Immunogen
Synthetic peptide corresponding to Human PPAR alpha aa 450 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab140319) -
Positive control
- This antibody gave a positive signal in the following whole cell lysates: HepG2; HEK293; NIH3T3; Jurkat.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Nucleotide metabolism
- Molecular processes
- Mitochondrial transcription
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab126285 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa). |
Target
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Function
Ligand-activated transcription factor. Key regulator of lipid metabolism. Activated by the endogenous ligand 1-palmitoyl-2-oleoyl-sn-glycerol-3-phosphocholine (16:0/18:1-GPC). Activated by oleylethanolamide, a naturally occurring lipid that regulates satiety (By similarity). Receptor for peroxisome proliferators such as hypolipidemic drugs and fatty acids. Regulates the peroxisomal beta-oxidation pathway of fatty acids. Functions as transcription activator for the ACOX1 and P450 genes. Transactivation activity requires heterodimerization with RXRA and is antagonized by NR2C2. -
Tissue specificity
Skeletal muscle, liver, heart and kidney. -
Sequence similarities
Belongs to the nuclear hormone receptor family. NR1 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 281992 Cow
- Entrez Gene: 403654 Dog
- Entrez Gene: 5465 Human
- Entrez Gene: 19013 Mouse
- Entrez Gene: 25747 Rat
- Omim: 170998 Human
- SwissProt: Q95N78 Dog
- SwissProt: Q07869 Human
see all -
Alternative names
- hPPAR antibody
- MGC2237 antibody
- MGC2452 antibody
see all
Images
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Western blot - Anti-PPAR alpha antibody (ab126285)All lanes : Anti-PPAR alpha antibody (ab126285) at 1 µg/ml
Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 4 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 52 kDa
Observed band size: 52 kDa
Additional bands at: 37 kDa, 50 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 1 minute
Protocols
Datasheets and documents
References
This product has been referenced in:
- Guillory B et al. Ghrelin deletion protects against age-associated hepatic steatosis by downregulating the C/EBPa-p300/DGAT1 pathway. Aging Cell 17:N/A (2018). WB ; Mouse . Read more (PubMed: 29024407) »
- Zhu Y et al. Expression patterns of hepatic genes involved in lipid metabolism in cows with subclinical or clinical ketosis. J Dairy Sci N/A:N/A (2018). Read more (PubMed: 30471902) »
