Overview

  • Product name

  • Description

    Rabbit polyclonal to PPAR alpha
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Rabbit, Human
    Predicted to work with: Rat, Cow, Dog, Macaque monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human PPAR alpha aa 450 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab140319)

  • Positive control

    • This antibody gave a positive signal in the following whole cell lysates: HepG2; HEK293; NIH3T3; Jurkat.

Properties

Applications

Our Abpromise guarantee covers the use of ab126285 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).

Target

  • Function

    Ligand-activated transcription factor. Key regulator of lipid metabolism. Activated by the endogenous ligand 1-palmitoyl-2-oleoyl-sn-glycerol-3-phosphocholine (16:0/18:1-GPC). Activated by oleylethanolamide, a naturally occurring lipid that regulates satiety (By similarity). Receptor for peroxisome proliferators such as hypolipidemic drugs and fatty acids. Regulates the peroxisomal beta-oxidation pathway of fatty acids. Functions as transcription activator for the ACOX1 and P450 genes. Transactivation activity requires heterodimerization with RXRA and is antagonized by NR2C2.
  • Tissue specificity

    Skeletal muscle, liver, heart and kidney.
  • Sequence similarities

    Belongs to the nuclear hormone receptor family. NR1 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • hPPAR antibody
    • MGC2237 antibody
    • MGC2452 antibody
    • NR1C1 antibody
    • Nuclear receptor subfamily 1 group C member 1 antibody
    • OTTHUMP00000197740 antibody
    • OTTHUMP00000197741 antibody
    • Peroxisome proliferative activated receptor alpha antibody
    • Peroxisome proliferator activated receptor alpha antibody
    • Peroxisome proliferator-activated receptor alpha antibody
    • PPAR antibody
    • PPAR-alpha antibody
    • ppara antibody
    • PPARA_HUMAN antibody
    • PPARalpha antibody
    see all

Images

  • All lanes : Anti-PPAR alpha antibody (ab126285) at 1 µg/ml

    Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 4 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 52 kDa
    Observed band size: 52 kDa
    Additional bands at: 37 kDa, 50 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 1 minute

References

This product has been referenced in:

  • Guillory B  et al. Ghrelin deletion protects against age-associated hepatic steatosis by downregulating the C/EBPa-p300/DGAT1 pathway. Aging Cell 17:N/A (2018). WB ; Mouse . Read more (PubMed: 29024407) »
  • Zhu Y  et al. Expression patterns of hepatic genes involved in lipid metabolism in cows with subclinical or clinical ketosis. J Dairy Sci N/A:N/A (2018). Read more (PubMed: 30471902) »
See all 2 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Western blot
Loading amount
40 µg
Gel Running Conditions
Reduced Denaturing (7.5)
Sample
Rabbit Tissue lysate - whole (liver)
Specification
liver
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 7.5% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Jun 25 2013

Answer

Perdona por la espera.

He estado consultando las posibilidades de reaccionar con lisados celulares de conejos entre los anticuerpos contra PPAR alpha que habíamos comentado.

Tengo buenas noticias, pues varios de ellos muestran porcentajes altos de homología con la proteína en conejo. De todos ellos, quizás el que más posibilidades tiene de funcionar en las condiciones que buscáis sea ab126285.

Si estas interesada en comprar el anticuerpo y participar en el sistema Abreview de evaluación de anticuerpos, no dudes en comentármelo para que primero te envíe el código canjeable por el importe del anticuerpo.

Espero entonces tu respuesta. Que pases una buena tarde.

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