Overview

  • Product name
  • Description
    Rabbit polyclonal to PPAR alpha
  • Host species
    Rabbit
  • Specificity
    The antibody recognizes ~52 kDa PPARa in human sample. A ~28 kDa cleavage fragment can also be detected in samples of human, mouse, and rat origins.
  • Tested applications
    Suitable for: IP, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Dog
  • Immunogen

    Synthetic peptide surrounding amino acid 25 of human PPARa.

  • Positive control
    • Rat liver/ kidney Baboon myometrium single band at ~52 kDa. K562 cells three bands: 52 kDa band and ~60 kDa band have the similar density and a much weaker band at ~45-50 kDa.

Applications

Our Abpromise guarantee covers the use of ab24509 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 12.5-1000 µg/mg of lysate.
WB Use a concentration of 3 - 6 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).

Target

  • Function
    Ligand-activated transcription factor. Key regulator of lipid metabolism. Activated by the endogenous ligand 1-palmitoyl-2-oleoyl-sn-glycerol-3-phosphocholine (16:0/18:1-GPC). Activated by oleylethanolamide, a naturally occurring lipid that regulates satiety (By similarity). Receptor for peroxisome proliferators such as hypolipidemic drugs and fatty acids. Regulates the peroxisomal beta-oxidation pathway of fatty acids. Functions as transcription activator for the ACOX1 and P450 genes. Transactivation activity requires heterodimerization with RXRA and is antagonized by NR2C2.
  • Tissue specificity
    Skeletal muscle, liver, heart and kidney.
  • Sequence similarities
    Belongs to the nuclear hormone receptor family. NR1 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • hPPAR antibody
    • MGC2237 antibody
    • MGC2452 antibody
    • NR1C1 antibody
    • Nuclear receptor subfamily 1 group C member 1 antibody
    • OTTHUMP00000197740 antibody
    • OTTHUMP00000197741 antibody
    • Peroxisome proliferative activated receptor alpha antibody
    • Peroxisome proliferator activated receptor alpha antibody
    • Peroxisome proliferator-activated receptor alpha antibody
    • PPAR antibody
    • PPAR-alpha antibody
    • ppara antibody
    • PPARA_HUMAN antibody
    • PPARalpha antibody
    see all

Images

  • All lanes : Anti-PPAR alpha antibody (ab24509) at 0.5 µg/ml

    Lane 1 : Jurkat Cells
    Lane 2 : Jurkat Cells
    Lane 3 : Rat Liver
    Lane 4 : Rat Kidney

    Predicted band size: 52 kDa

References

This product has been referenced in:
  • Mukherjee S  et al. Boosting efferocytosis in alveolar space using BCG vaccine to protect host against influenza pneumonia. PLoS One 12:e0180143 (2017). WB ; Mouse . Read more (PubMed: 28686604) »
  • Fu Y  et al. Tribbles homolog 1 enhances cholesterol efflux from oxidized low-density lipoprotein-loaded THP-1 macrophages. Exp Ther Med 14:862-866 (2017). Read more (PubMed: 28673011) »
See all 16 Publications for this product

Customer reviews and Q&As

1-7 of 7 Abreviews or Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (mouse heart)
Loading amount
50 µg
Specification
mouse heart
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C

Abcam user community

Verified customer

Submitted Jun 23 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (C2C12)
Loading amount
40 µg
Specification
C2C12
Gel Running Conditions
Reduced Denaturing
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Nov 07 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunoprecipitation
Sample
Mouse Cell lysate - whole cell (Hepa1-6 (murine hepatoma) cells)
Total protein in input
1000 µg
Specification
Hepa1-6 (murine hepatoma) cells
Immuno-precipitation step
Protein A

Abcam user community

Verified customer

Submitted Apr 12 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Hepa1-6 (murine hepatoma) cells)
Loading amount
50 µg
Specification
Hepa1-6 (murine hepatoma) cells
Gel Running Conditions
Reduced Denaturing
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Apr 12 2011

Answer

Thank you for getting back to me. From literature searching I can see that the detection of PPAR alpha in rat muscle is to be expected. Given that your customer has been preparing their samples in the same manner and has been detecting the non specific band at ~60 kDa in all of their samples I find it concerning that they have been unable to detect a 50KDa band in their skeletal muscle samples. After consideration I am prepared to offer your customer credit against this purchase provided that they purchased the antibody within the past 90 days. If this is the case please email me details of the initial order including the order number and date of purchase.

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Answer

Thank you for your enquiry. I am sorry to hear that you have been having difficulties with this antibody. I have read your technical questionaire and I have a few comments. On the antibody datasheet it details that when using a human erythroid cell line (K562) three bands are detected: 52 kDa and ~60 kDa band of similar densities and a much weaker band at ~45-50 kDa. Given your customers observations I consider it very likely that the three bands that they have been detecting in rat cells are the three bands mentioned. The minor deviation in molecular weight (50KDa as opposed to 52KDa) is most likely attributable to different molecular weight standards employed in each case. It is well known that commercially available molecular weight standards migrate faster than others. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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