Anti-PPAR alpha antibody - ChIP Grade (ab227074)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-PPAR alpha antibody - ChIP Grade
See all PPAR alpha primary antibodies -
Description
Rabbit polyclonal to PPAR alpha - ChIP Grade -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, ChIPmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Dog, Pig, Rhesus monkey
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Immunogen
Recombinant fragment within Human PPAR alpha (N terminal). The exact sequence is proprietary.
Database link: Q07869 -
Positive control
- ChIP: HepG2 chromatin extract. IP: HepG2 whole cell lysate (ab7900). WB: Rat primary hepatocyte lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Nucleotide metabolism
- Molecular processes
- Mitochondrial transcription
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab227074 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 52 kDa. | |
| IP | 1/100 - 1/500. | |
| ChIP | Use at an assay dependent concentration. Use 5 μg per reaction. |
Target
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Function
Ligand-activated transcription factor. Key regulator of lipid metabolism. Activated by the endogenous ligand 1-palmitoyl-2-oleoyl-sn-glycerol-3-phosphocholine (16:0/18:1-GPC). Activated by oleylethanolamide, a naturally occurring lipid that regulates satiety (By similarity). Receptor for peroxisome proliferators such as hypolipidemic drugs and fatty acids. Regulates the peroxisomal beta-oxidation pathway of fatty acids. Functions as transcription activator for the ACOX1 and P450 genes. Transactivation activity requires heterodimerization with RXRA and is antagonized by NR2C2. -
Tissue specificity
Skeletal muscle, liver, heart and kidney. -
Sequence similarities
Belongs to the nuclear hormone receptor family. NR1 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 403654 Dog
- Entrez Gene: 5465 Human
- Entrez Gene: 397239 Pig
- Entrez Gene: 25747 Rat
- Entrez Gene: 613250 Rhesus monkey
- Omim: 170998 Human
- SwissProt: Q95N78 Dog
- SwissProt: Q07869 Human
see all -
Alternative names
- hPPAR antibody
- MGC2237 antibody
- MGC2452 antibody
see all
Images
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Western blot - Anti-PPAR alpha antibody - ChIP Grade (ab227074)Anti-PPAR alpha antibody - ChIP Grade (ab227074) at 1/1000 dilution + Rat primary hepatocyte lysate at 30 µg
Secondary
HRP-conjugated anti-rabbit IgG
Predicted band size: 52 kDa7.5% SDS-PAGE gel.
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ChIP - Anti-PPAR alpha antibody - ChIP Grade (ab227074)Cross-linked ChIP was performed with HepG2 (human liver hepatocellular carcinoma cell line) chromatin extract and 5 μg of either control rabbit IgG or ab227074. The precipitated DNA was detected by PCR with primer set targeting to C3 promotor or NOD1 gene.
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Immunoprecipitation - Anti-PPAR alpha antibody - ChIP Grade (ab227074)PPAR alpha was immunoprecipitated from HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract with ab227074 at 1/100 dilution. Western blot was performed from the immunoprecipitate using ab227074 at 1/1000 dilution.
Lane 1: Control IgG IP in HepG2 whole cell extract.
Lane 2: ab227074 IP in HepG2 whole cell extract.
Datasheets and documents
References
ab227074 has not yet been referenced specifically in any publications.
