Overview

  • Product name

    Anti-PPAR alpha antibody - ChIP Grade
    See all PPAR alpha primary antibodies
  • Description

    Rabbit polyclonal to PPAR alpha - ChIP Grade
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, ChIPmore details
  • Species reactivity

    Reacts with: Rat, Human
    Predicted to work with: Dog, Pig, Rhesus monkey
  • Immunogen

    Recombinant fragment within Human PPAR alpha (N terminal). The exact sequence is proprietary.
    Database link: Q07869

  • Positive control

    • ChIP: HepG2 chromatin extract. IP: HepG2 whole cell lysate (ab7900). WB: Rat primary hepatocyte lysate.

Applications

Our Abpromise guarantee covers the use of ab227074 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 52 kDa.
IP 1/100 - 1/500.
ChIP Use at an assay dependent concentration.

Use 5 μg per reaction.

Target

  • Function

    Ligand-activated transcription factor. Key regulator of lipid metabolism. Activated by the endogenous ligand 1-palmitoyl-2-oleoyl-sn-glycerol-3-phosphocholine (16:0/18:1-GPC). Activated by oleylethanolamide, a naturally occurring lipid that regulates satiety (By similarity). Receptor for peroxisome proliferators such as hypolipidemic drugs and fatty acids. Regulates the peroxisomal beta-oxidation pathway of fatty acids. Functions as transcription activator for the ACOX1 and P450 genes. Transactivation activity requires heterodimerization with RXRA and is antagonized by NR2C2.
  • Tissue specificity

    Skeletal muscle, liver, heart and kidney.
  • Sequence similarities

    Belongs to the nuclear hormone receptor family. NR1 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • hPPAR antibody
    • MGC2237 antibody
    • MGC2452 antibody
    • NR1C1 antibody
    • Nuclear receptor subfamily 1 group C member 1 antibody
    • OTTHUMP00000197740 antibody
    • OTTHUMP00000197741 antibody
    • Peroxisome proliferative activated receptor alpha antibody
    • Peroxisome proliferator activated receptor alpha antibody
    • Peroxisome proliferator-activated receptor alpha antibody
    • PPAR antibody
    • PPAR-alpha antibody
    • ppara antibody
    • PPARA_HUMAN antibody
    • PPARalpha antibody
    see all

Images

  • Anti-PPAR alpha antibody - ChIP Grade (ab227074) at 1/1000 dilution + Rat primary hepatocyte lysate at 30 µg

    Secondary
    HRP-conjugated anti-rabbit IgG

    Predicted band size: 52 kDa



    7.5% SDS-PAGE gel.

  • Cross-linked ChIP was performed with HepG2 (human liver hepatocellular carcinoma cell line) chromatin extract and 5 μg of either control rabbit IgG or ab227074. The precipitated DNA was detected by PCR with primer set targeting to C3 promotor or NOD1 gene.

  • PPAR alpha was immunoprecipitated from HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract with ab227074 at 1/100 dilution. Western blot was performed from the immunoprecipitate using ab227074 at 1/1000 dilution.

    Lane 1: Control IgG IP in HepG2 whole cell extract.

    Lane 2: ab227074 IP in HepG2 whole cell extract.

References

ab227074 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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