Anti-PPAR alpha antibody - ChIP Grade (ab227074)
Key features and details
- Rabbit polyclonal to PPAR alpha - ChIP Grade
- Suitable for: WB, IP, ChIP
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-PPAR alpha antibody - ChIP Grade
See all PPAR alpha primary antibodies -
Description
Rabbit polyclonal to PPAR alpha - ChIP Grade -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, ChIPmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Dog, Pig, Rhesus monkey
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Immunogen
Recombinant fragment within Human PPAR alpha (N terminal). The exact sequence is proprietary.
Database link: Q07869 -
Positive control
- ChIP: HepG2 chromatin extract. IP: HepG2 whole cell lysate (ab7900). WB: Rat primary hepatocyte lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Nucleotide metabolism
- Molecular processes
- Mitochondrial transcription
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab227074 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
1/500 - 1/3000. Predicted molecular weight: 52 kDa.
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| IP |
1/100 - 1/500.
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| ChIP |
Use at an assay dependent concentration.
Use 5 μg per reaction. |
| Notes |
|---|
|
WB
1/500 - 1/3000. Predicted molecular weight: 52 kDa. |
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IP
1/100 - 1/500. |
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ChIP
Use at an assay dependent concentration. Use 5 μg per reaction. |
Target
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Function
Ligand-activated transcription factor. Key regulator of lipid metabolism. Activated by the endogenous ligand 1-palmitoyl-2-oleoyl-sn-glycerol-3-phosphocholine (16:0/18:1-GPC). Activated by oleylethanolamide, a naturally occurring lipid that regulates satiety (By similarity). Receptor for peroxisome proliferators such as hypolipidemic drugs and fatty acids. Regulates the peroxisomal beta-oxidation pathway of fatty acids. Functions as transcription activator for the ACOX1 and P450 genes. Transactivation activity requires heterodimerization with RXRA and is antagonized by NR2C2. -
Tissue specificity
Skeletal muscle, liver, heart and kidney. -
Sequence similarities
Belongs to the nuclear hormone receptor family. NR1 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 403654 Dog
- Entrez Gene: 5465 Human
- Entrez Gene: 397239 Pig
- Entrez Gene: 25747 Rat
- Entrez Gene: 613250 Rhesus monkey
- Omim: 170998 Human
- SwissProt: Q95N78 Dog
- SwissProt: Q07869 Human
see all -
Alternative names
- hPPAR antibody
- MGC2237 antibody
- MGC2452 antibody
see all
Images
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Western blot - Anti-PPAR alpha antibody - ChIP Grade (ab227074)Anti-PPAR alpha antibody - ChIP Grade (ab227074) at 1/1000 dilution + Rat primary hepatocyte lysate at 30 µg
Secondary
HRP-conjugated anti-rabbit IgG
Predicted band size: 52 kDa7.5% SDS-PAGE gel.
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ChIP - Anti-PPAR alpha antibody - ChIP Grade (ab227074)Cross-linked ChIP was performed with HepG2 (human liver hepatocellular carcinoma cell line) chromatin extract and 5 μg of either control rabbit IgG or ab227074. The precipitated DNA was detected by PCR with primer set targeting to C3 promotor or NOD1 gene.
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Immunoprecipitation - Anti-PPAR alpha antibody - ChIP Grade (ab227074)PPAR alpha was immunoprecipitated from HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract with ab227074 at 1/100 dilution. Western blot was performed from the immunoprecipitate using ab227074 at 1/1000 dilution.
Lane 1: Control IgG IP in HepG2 whole cell extract.
Lane 2: ab227074 IP in HepG2 whole cell extract.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab227074 has been referenced in 4 publications.
- Dai M et al. Icariin enhance mild hypothermia-induced neuroprotection via inhibiting the activation of NF-?B in experimental ischemic stroke. Metab Brain Dis N/A:N/A (2021). PubMed: 33978900
- Murphy SA et al. PGC1/PPAR drive cardiomyocyte maturation at single cell level via YAP1 and SF3B2. Nat Commun 12:1648 (2021). PubMed: 33712605
- Li J et al. Angiotensin II-induced muscle atrophy via PPAR? suppression is mediated by miR-29b. Mol Ther Nucleic Acids 23:743-756 (2021). PubMed: 33614226
- Wang C et al. CTRP13 Preserves Endothelial Function by Targeting GTP Cyclohydrolase 1 in Diabetes. Diabetes 69:99-111 (2020). PubMed: 31676569