Recombinant Anti-PPAR alpha antibody [EPR21244] (ab245119)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPAR alpha antibody [EPR21244] (ab245119)](https://www.abcam.com/ps/products/245/ab245119/Images/ab245119-336252-anti-ppar-alpha-antibody-epr21244-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPAR alpha antibody [EPR21244] (ab245119)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR21244] to PPAR alpha
- Suitable for: IHC-P
- Reacts with: Human
Overview
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Product name
Anti-PPAR alpha antibody [EPR21244]
See all PPAR alpha primary antibodies -
Description
Rabbit monoclonal [EPR21244] to PPAR alpha -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Pmore details
Unsuitable for: WB -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is considered to be commercially sensitive.
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Positive control
- IHC-P: Hu Duodenum
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General notes
This product was made using synthetic libraries and phage display technology.
This antibody is a recombinant chimeric antibody. Rabbit chimeric monoclonal antibody (Human Fab/Rabbit Fc).
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
Clonality
Monoclonal -
Clone number
EPR21244 -
Isotype
IgG1 -
Research areas
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Nucleotide metabolism
- Molecular processes
- Mitochondrial transcription
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
Associated products
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Compatible Secondaries
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab245119 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
Use a concentration of 0.03 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| Notes |
|---|
|
IHC-P
Use a concentration of 0.03 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Ligand-activated transcription factor. Key regulator of lipid metabolism. Activated by the endogenous ligand 1-palmitoyl-2-oleoyl-sn-glycerol-3-phosphocholine (16:0/18:1-GPC). Activated by oleylethanolamide, a naturally occurring lipid that regulates satiety (By similarity). Receptor for peroxisome proliferators such as hypolipidemic drugs and fatty acids. Regulates the peroxisomal beta-oxidation pathway of fatty acids. Functions as transcription activator for the ACOX1 and P450 genes. Transactivation activity requires heterodimerization with RXRA and is antagonized by NR2C2. -
Tissue specificity
Skeletal muscle, liver, heart and kidney. -
Sequence similarities
Belongs to the nuclear hormone receptor family. NR1 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 5465 Human
- Omim: 170998 Human
- SwissProt: Q07869 Human
- Unigene: 103110 Human
- Unigene: 710044 Human
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Alternative names
- hPPAR antibody
- MGC2237 antibody
- MGC2452 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPAR alpha antibody [EPR21244] (ab245119)
IHC image of PPAR alpha staining in a section of formalin-fixed paraffin-embedded normal human duodenum*, performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab245119 at 0.03μg/ml concentration, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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![Anti-PPAR alpha antibody [EPR21244] (ab245119)](https://www.abcam.com/ps/products/245/ab245119/Images/ab245119-3-benefits-of-recombinant-antibodies.png)
Anti-PPAR alpha antibody [EPR21244] (ab245119)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (1)
ab245119 has been referenced in 1 publication.
- Liu B et al. Polygonatum sibiricum F. Delaroche polysaccharide ameliorates HFD-induced mouse obesity via regulation of lipid metabolism and inflammatory response. Mol Med Rep 24:N/A (2021). PubMed: 33982779
