Overview

  • Product name

    PPAR (alpha, delta, gamma) Transcription Factor Assay Kit
  • Detection method

    Colorimetric
  • Sample type

    Adherent cells, Suspension cells, Nuclear Extracts
  • Assay type

    Semi-quantitative
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mammals
  • Product overview

    Abcam's PPAR Transcription Factor Assay Kit (ab133113) is a non-radioactive, sensitive method for detecting specific transcription factor DNA binding activity in nuclear extracts and whole cell lysates.


    A 96-well enzyme-linked immunosorbent assay (ELISA) replaces the cumbersome radioactive electrophoretic mobility shift assay (EMSA). A specific double stranded DNA (dsDNA) sequence containing the PPAR response element is immobilized onto the bottom of wells of a 96-well plate. PPARs contained in a nuclear extract, bind specifically to the PPAR response element. PPAR alpha, delta, or gamma are detected by addition of specific primary antibodies directed against the individual PPARs. A secondary antibody conjugated to HRP is added to provide a sensitive colorimetric readout at 450 nm. ab133113 comes with a single plate that measures all three isoforms of PPAR alpha, delta, and gamma. There are enough reagents for one-third of a plate for each isoform.

  • Notes

    Peroxisome proliferator-activated receptors (PPARs) are ligand activated nuclear receptors. Three PPAR subtypes have been identified: alpha, delta and gamma. PPARs can be activated by polyunsaturated fatty acids, eicosanoids and various synthtic ligands. PPARs play pivotal roles in the regulation of lipid metabolism and homeostasis and are important indirect and direct regulators of cellular insulin sensitivity.

  • Platform

    Microplate reader

Properties

Images

  • Increasing amounts of positive control (total lysate) are assayed for PPAR gamma DNA-binding activity using ab133113.
  • Increasing amounts of positive control (total lysate) are assayed for PPAR delta DNA-binding activity using ab133113.
  • Increasing amounts of positive control (total lysate) are assayed for PPAR alpha DNA-binding activity using ab133113.

Protocols

References

This product has been referenced in:

  • Meex RC  et al. ATGL-mediated triglyceride turnover and the regulation of mitochondrial capacity in skeletal muscle. Am J Physiol Endocrinol Metab 308:E960-70 (2015). Functional Studies . Read more (PubMed: 25852007) »
See 1 Publication for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

There is a control protein for PARP delta included with this kit. Due to an IT issue this component did not make it on to the online product datasheet. This should be updated shortly.

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Question
Answer


According to the datasheet, the transcription factor stop solution is the only component to contain sulphuric acid.
I can confirm that the transcription factor stop solution contains 12 ml of a 0.5 M sulphuric acid solution.

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