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Follow up to previous correspondance: Thank you for your suggestions. I was able to get a signal with all of the antibodies, but I am worried about the sizes of the bands. According to your data sheet, the size of ALL the bands is about 52 kD. However, only the PPARalpha (ab8934) detects a specific band of this size. The bands of the phosphorylated forms of PPARalpha (antibodies ab3484 and ab3485)are closer to 70 kD, but are quite specific, producing little background except during long exposures (1h). Are you completely sure that the sizes of the phosphorylated forms should be approx. 52 kD, or is it possible that they are larger, i.e. the 70 kD bands? I hope you are able to answer this last query, as I want to ascertain that the signal we are getting is the proper one. For you question concerning storage, the antibodies were aliquoted and stored in -20 C immediately upon arrival.
Asked on Nov 09 2004
Thank you for your recent e-mail. My colleague Jennifer is unexpectedly away this week and I would like to help you. I understand you have recurring problems with ab3484 and ab3485 detecting the wrong bands and your protocol seems fine. I would like to send you replacements for these two antibodies as they should recognise well the 52kDa band. Can you please provide me with the order number used to purchase the antibodies and I will arrange replacements to be sent to you ASAP. I'm very sorry for the delay, thank you for your patience. I look forward to hearing from you soon,
Answered on Nov 16 2004