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    ppar-alpha-transcription-factor-assay-kit-ab133107.pdf

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Epigenetics and Nuclear Signaling Transcription Domain Families Zinc Finger
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PPAR alpha Transcription Factor Assay Kit (ab133107)

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  • SDS
  • Protocol Booklet
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Functional Studies - PPAR alpha Transcription Factor Assay Kit (ab133107)

    Key features and details

    • Assay type: Semi-quantitative
    • Detection method: Colorimetric
    • Platform: Microplate reader
    • Sample type: Adherent cells, Nuclear Extracts, Suspension cells

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    Overview

    • Product name

      PPAR alpha Transcription Factor Assay Kit
    • Detection method

      Colorimetric
    • Sample type

      Adherent cells, Suspension cells, Nuclear Extracts
    • Assay type

      Semi-quantitative
    • Species reactivity

      Reacts with: Mouse, Rat, Human
    • Product overview

      Abcam's PPAR alpha Transcription Factor Assay Kit (ab133107) is a non-radioactive, sensitive method for detecting specific transcription factor DNA binding activity in nuclear extracts.

      A 96-well enzyme-linked immunosorbent assay (ELISA) replaces the cumbersome radioactive electrophoretic mobility shift assay (EMSA). A specific double stranded DNA (dsDNA) sequence containing the peroxisome proliferator response element (PPRE) is immobilized onto the bottom of wells of a 96-well plate. PPARs contained in a nuclear extract, bind specifically to the PPRE. PPAR alpha is detected by addition of specific primary antibody directed against PPAR alpha. A secondary antibody conjugated to HRP is added to provide a sensitive colorometric readout at 450 nm. PPAR alpha Transcription Factor Assay will not cross-react with PPAR gamma or PPAR delta.

    • Notes

      Peroxisome proliferator-activated receptors (PPARs) are ligand activated nuclear receptors. Three PPAR subtypes have been identified: alpha, delta and gamma. PPARs can be activated by polyunsaturated fatty acids, eicosanoids and various synthtic ligands.

      PPAR alpha is predominantly expressed in liver, heart and kidneys. Upon activation by ligands such as free fatty acids, lipids and other small molecules, PPAR alpha forms a heterodimeric complex with RXR alpha, and this complex will bind to response elements allowing transcription of target genes involved in lipid metabolism.

    • Platform

      Microplate reader

    Properties

    • Storage instructions

      Please refer to protocols.
    • Components 96 tests
      96-Well Plate Cover 1 unit
      Polysorbate 20 1 vial
      Transcription Factor Antibody Binding Buffer (10X) 1 x 3ml
      Transcription Factor Binding Assay Buffer (4X) 1 x 3ml
      Transcription Factor Developing Solution 1 x 12ml
      Transcription Factor Goat Anti-Rabbit HRP Conjugate 1 x 100µl
      Transcription Factor PPAR 96-Well Strip Plate 1 unit
      Transcription Factor PPAR alpha Positive Control 1 vial
      Transcription Factor PPAR alpha Primary Antibody 1 vial
      Transcription Factor PPAR Competitor dsDNA 1 vial
      Transcription Factor Reagent A 1 x 120µl
      Transcription Factor Stop Solution 1 x 12ml
      Wash Buffer Concentrate (400X) 1 x 5ml
    • Research areas

      • Epigenetics and Nuclear Signaling
      • Transcription
      • Domain Families
      • Zinc Finger
      • Cancer
      • Signal transduction
      • Nuclear signaling
      • Nuclear hormone receptors
      • Other
      • Cardiovascular
      • Lipids / Lipoproteins
      • Fatty Acids
      • Metabolism
      • Cancer
      • Cancer Metabolism
      • Metabolic signaling pathway
      • Metabolism of lipids and lipoproteins
      • Cancer
      • Cancer Metabolism
      • Response to hypoxia
      • Kits/ Lysates/ Other
      • Kits
      • Cell Metabolism Kits
      • Lipid Metabolism Kits
      • Fatty acids
      • Kits/ Lysates/ Other
      • Kits
      • Cell Metabolism Kits
      • Lipid Metabolism Kits
      • Obesity
      • Metabolism
      • Pathways and Processes
      • Mitochondrial Metabolism
      • Mitochondrial Biogenesis
      • Metabolism
      • Pathways and Processes
      • Metabolic signaling pathways
      • Nucleotide metabolism
      • Molecular processes
      • Mitochondrial transcription
      • Metabolism
      • Pathways and Processes
      • Metabolic signaling pathways
      • Lipid and lipoprotein metabolism
      • Lipid metabolism
      • Metabolism
      • Pathways and Processes
      • Metabolic signaling pathways
      • Lipid and lipoprotein metabolism
      • Fatty acids
      • Metabolism
      • Pathways and Processes
      • Metabolism processes
      • Hypoxia
      • Metabolism
      • Types of disease
      • Obesity
      • Kits/ Lysates/ Other
      • Kits
      • Epigenetic kits
      • Transcription factor assay kits
      • Metabolism
      • Pathways and Processes
      • Redox metabolism
      • Fatty acid oxidation
      • Neuroscience
      • Processes
    • Function

      Ligand-activated transcription factor. Key regulator of lipid metabolism. Activated by the endogenous ligand 1-palmitoyl-2-oleoyl-sn-glycerol-3-phosphocholine (16:0/18:1-GPC). Activated by oleylethanolamide, a naturally occurring lipid that regulates satiety (By similarity). Receptor for peroxisome proliferators such as hypolipidemic drugs and fatty acids. Regulates the peroxisomal beta-oxidation pathway of fatty acids. Functions as transcription activator for the ACOX1 and P450 genes. Transactivation activity requires heterodimerization with RXRA and is antagonized by NR2C2.
    • Tissue specificity

      Skeletal muscle, liver, heart and kidney.
    • Sequence similarities

      Belongs to the nuclear hormone receptor family. NR1 subfamily.
      Contains 1 nuclear receptor DNA-binding domain.
    • Cellular localization

      Nucleus.
    • Target information above from: UniProt accession Q07869 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Alternative names

      • hPPAR
      • MGC2237
      • MGC2452
      • NR1C1
      • Nuclear receptor subfamily 1 group C member 1
      • OTTHUMP00000197740
      • OTTHUMP00000197741
      • Peroxisome proliferative activated receptor alpha
      • Peroxisome proliferator activated receptor alpha
      • Peroxisome proliferator-activated receptor alpha
      • PPAR
      • PPAR-alpha
      • ppara
      • PPARA_HUMAN
      • PPARalpha
      see all
    • Database links

      • Entrez Gene: 5465 Human
      • Entrez Gene: 19013 Mouse
      • Entrez Gene: 25747 Rat
      • Omim: 170998 Human
      • SwissProt: Q07869 Human
      • SwissProt: P23204 Mouse
      • SwissProt: P37230 Rat
      • Unigene: 103110 Human
      • Unigene: 710044 Human
      • Unigene: 212789 Mouse
      • Unigene: 9753 Rat
      see all

    Associated products

    • Related Products

      • Indomethacin, Non-selective COX inhibitor (ab120719)
      • Rosiglitazone, PPARgamma agonist (ab120762)
      • Pioglitazone hydrochloride, PPAR-gamma agonist (ab120794)
      • Telmisartan, angiotensin II (AT1) antagonist (ab120831)
      • Clofibric acid, PPARalpha agonist (ab120833)
      • GW 9662, PPARgamma antagonist (ab141125)
      • Ciglitazone, PPARgamma agonist (ab141139)
      • MK886, Lipoxygenase inhibitor (ab141140)
      • 15-Deoxy-Delta12,14-prostaglandin J2, Selective PPARgamma agonist (ab141717)
    • sELISA pair antibody

      • Nuclear Extraction Kit (ab113474)

    Images

    • Functional Studies - PPAR alpha Transcription Factor Assay Kit (ab133107)
      Functional Studies - PPAR alpha Transcription Factor Assay Kit (ab133107)
      Panel A: Increasing amounts of positive control (total lysate) are assayed for PPAR alpha DNA-binding activity using ab133107.
      Panel B: PPAR alpha DNA-binding assays are performed in the presence of competitive dsDNA. The decrease in signal caused by addition of competitive dsDNA confirms the assay specificity.

    Protocols

    • Protocol Booklet

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (5)

    Publishing research using ab133107? Please let us know so that we can cite the reference in this datasheet.

    ab133107 has been referenced in 5 publications.

    • Watts AJ  et al. Regulation of Peroxisome Proliferator-Activated Receptor Pathway During Torpor in the Garden Dormouse, Eliomys quercinus. Front Physiol 11:615025 (2020). PubMed: 33408645
    • Ghosh S  et al. Linoleic acid in diets of mice increases total endocannabinoid levels in bowel and liver: modification by dietary glucose. Obes Sci Pract 5:383-394 (2019). PubMed: 31452923
    • Qi K  et al. Tongxinluo attenuates reperfusion injury in diabetic hearts by angiopoietin-like 4-mediated protection of endothelial barrier integrity via PPAR-a pathway. PLoS One 13:e0198403 (2018). PubMed: 29912977
    • Ren T  et al. The Combination of Blueberry Juice and Probiotics Ameliorate Non-Alcoholic Steatohepatitis (NASH) by Affecting SREBP-1c/PNPLA-3 Pathway via PPAR-a. Nutrients 9:N/A (2017). PubMed: 28264426
    • Bakshi MV  et al. Total body exposure to low-dose ionizing radiation induces long-term alterations to the liver proteome of neonatally exposed mice. J Proteome Res 14:366-73 (2015). PubMed: 25299163

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