PPAR alpha Transcription Factor Assay Kit (ab133107)

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Functional Studies - PPAR alpha Transcription Factor Assay Kit (ab133107)

    Key features and details

    • Assay type: Semi-quantitative
    • Detection method: Colorimetric
    • Platform: Microplate reader
    • Sample type: Adherent cells, Nuclear Extracts, Suspension cells

    Overview

    • Product name

      PPAR alpha Transcription Factor Assay Kit

    • Detection method

      Colorimetric

    • Sample type

      Adherent cells, Suspension cells, Nuclear Extracts

    • Assay type

      Semi-quantitative

    • Species reactivity

      Reacts with: Mouse, Rat, Human

    • Product overview

      Abcam's PPAR alpha Transcription Factor Assay Kit (ab133107) is a non-radioactive, sensitive method for detecting specific transcription factor DNA binding activity in nuclear extracts.

      A 96-well enzyme-linked immunosorbent assay (ELISA) replaces the cumbersome radioactive electrophoretic mobility shift assay (EMSA). A specific double stranded DNA (dsDNA) sequence containing the peroxisome proliferator response element (PPRE) is immobilized onto the bottom of wells of a 96-well plate. PPARs contained in a nuclear extract, bind specifically to the PPRE. PPAR alpha is detected by addition of specific primary antibody directed against PPAR alpha. A secondary antibody conjugated to HRP is added to provide a sensitive colorometric readout at 450 nm. PPAR alpha Transcription Factor Assay will not cross-react with PPAR gamma or PPAR delta.

    • Notes

      Peroxisome proliferator-activated receptors (PPARs) are ligand activated nuclear receptors. Three PPAR subtypes have been identified: alpha, delta and gamma. PPARs can be activated by polyunsaturated fatty acids, eicosanoids and various synthtic ligands.

      PPAR alpha is predominantly expressed in liver, heart and kidneys. Upon activation by ligands such as free fatty acids, lipids and other small molecules, PPAR alpha forms a heterodimeric complex with RXR alpha, and this complex will bind to response elements allowing transcription of target genes involved in lipid metabolism.

    • Platform

      Microplate reader

    Properties

    Images

    • Functional Studies - PPAR alpha Transcription Factor Assay Kit (ab133107)
      Functional Studies - PPAR alpha Transcription Factor Assay Kit (ab133107)
      Panel A: Increasing amounts of positive control (total lysate) are assayed for PPAR alpha DNA-binding activity using ab133107.
      Panel B: PPAR alpha DNA-binding assays are performed in the presence of competitive dsDNA. The decrease in signal caused by addition of competitive dsDNA confirms the assay specificity.

    References (5)

    Publishing research using ab133107? Please let us know so that we can cite the reference in this datasheet.

    ab133107 has been referenced in 5 publications.

    • Watts AJ  et al. Regulation of Peroxisome Proliferator-Activated Receptor Pathway During Torpor in the Garden Dormouse, Eliomys quercinus. Front Physiol 11:615025 (2020). PubMed: 33408645
    • Ghosh S  et al. Linoleic acid in diets of mice increases total endocannabinoid levels in bowel and liver: modification by dietary glucose. Obes Sci Pract 5:383-394 (2019). PubMed: 31452923
    • Qi K  et al. Tongxinluo attenuates reperfusion injury in diabetic hearts by angiopoietin-like 4-mediated protection of endothelial barrier integrity via PPAR-a pathway. PLoS One 13:e0198403 (2018). PubMed: 29912977
    • Ren T  et al. The Combination of Blueberry Juice and Probiotics Ameliorate Non-Alcoholic Steatohepatitis (NASH) by Affecting SREBP-1c/PNPLA-3 Pathway via PPAR-a. Nutrients 9:N/A (2017). PubMed: 28264426
    • Bakshi MV  et al. Total body exposure to low-dose ionizing radiation induces long-term alterations to the liver proteome of neonatally exposed mice. J Proteome Res 14:366-73 (2015). PubMed: 25299163

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