Overview

  • Product name

  • Description

    Rabbit polyclonal to PPAR delta
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow, Dog, Pig
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 129-378 of Human PPAR delta.

  • Positive control

    • Molt-4 and Mouse brown adipose tissue whole cell lysates; A549 Xenograft tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab137724 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 50 kDa.
IHC-P 1/100 - 1/1000.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)

Blocking condition: 

Incubate the sections in 3% H2O2 at room temperature for 30 minutes.

Incubate the sections in 1.5% goat serum (dilute goat serum by 1xPBS) at room temperature for 30 minutes.

Target

  • Function

    Ligand-activated transcription factor. Receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. Has a preference for poly-unsaturated fatty acids, such as gamma-linoleic acid and eicosapentanoic acid. Once activated by a ligand, the receptor binds to promoter elements of target genes. Regulates the peroxisomal beta-oxidation pathway of fatty acids. Functions as transcription activator for the acyl-CoA oxidase gene. Decreases expression of NPC1L1 once activated by a ligand.
  • Tissue specificity

    Ubiquitous with maximal levels in placenta and skeletal muscle.
  • Sequence similarities

    Belongs to the nuclear hormone receptor family. NR1 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • FAAR antibody
    • MGC3931 antibody
    • NR1C2 antibody
    • NUC1 antibody
    • NUCI antibody
    • NUCII antibody
    • Nuclear hormone receptor 1 antibody
    • Nuclear receptor subfamily 1 group C member 2 antibody
    • Peroxisome proliferative activated receptor delta antibody
    • Peroxisome proliferator-activated receptor beta (PPAR-beta) antibody
    • Peroxisome proliferator-activated receptor beta antibody
    • Peroxisome proliferator-activated receptor delta antibody
    • PPAR beta antibody
    • PPAR-beta antibody
    • PPAR-delta antibody
    • PPAR-ß antibody
    • PPARB antibody
    • ppard antibody
    • PPARD_HUMAN antibody
    see all

Images

  • Anti-PPAR delta antibody (ab137724) at 1/1000 dilution + Molt4 whole cell lysate at 30 µg

    Predicted band size: 50 kDa



    10% SDS PAGE
  • Anti-PPAR delta antibody (ab137724) at 1/1000 dilution + Mouse brown adipose tissue whole cell lysate
    at 50 µg

    Predicted band size: 50 kDa



    10% SDS PAGE
  • Immunohistochemical analysis of paraffin-embedded A549 xenograft tissue labelling PPAR delta with ab137724 at 1/100 dilution.

References

This product has been referenced in:

  • Maurya SK  et al. Sarcolipin Signaling Promotes Mitochondrial Biogenesis and Oxidative Metabolism in Skeletal Muscle. Cell Rep 24:2919-2931 (2018). Read more (PubMed: 30208317) »
  • Li XH  et al. Neuroprotective effects of sodium hydrosulfide against ß-amyloid-induced neurotoxicity. Int J Mol Med 38:1152-60 (2016). WB ; Rat . Read more (PubMed: 27511125) »
See all 3 Publications for this product

Customer reviews and Q&As

Filter by Application

Filter by Species

Filter by Ratings

Application
Western blot
Sample
Pig Cell lysate - whole cell (RPE)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
20 µg
Specification
RPE
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

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Verified customer

Submitted Jun 29 2017

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