Western blot abreview for Anti-PPAR delta antibody

Excellent
Abreviews
Application
Western blot
Sample
Pig Cell lysate - whole cell (RPE)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
20 µg
Specification
RPE
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/1000

Secondary antibody

Dilution
1/10000
Name
Non-Abcam antibody was used: Secondary antibody goat anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL
Exposure
20 second(s)
Bands
Specific: 50 kDa

Abcam user community

Verified customer

Submitted Jun 29 2017

Sign up