Overview

  • Product name

  • Description

    Goat polyclonal to PPAR delta
  • Host species

    Goat
  • Specificity

    ab21209 recognises isoform 1 of PPAR delta, but not isoform 2, a shorter protein with a different C-terminus.
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow, Pig
  • Immunogen

    Synthetic peptide corresponding to Human PPAR delta aa 429-441 (C terminal).
    Sequence:

    CHPLLQEIYKDMY

  • Positive control

    • WB: MOLT4 cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab21209 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 50 kDa).

Target

  • Function

    Ligand-activated transcription factor. Receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. Has a preference for poly-unsaturated fatty acids, such as gamma-linoleic acid and eicosapentanoic acid. Once activated by a ligand, the receptor binds to promoter elements of target genes. Regulates the peroxisomal beta-oxidation pathway of fatty acids. Functions as transcription activator for the acyl-CoA oxidase gene. Decreases expression of NPC1L1 once activated by a ligand.
  • Tissue specificity

    Ubiquitous with maximal levels in placenta and skeletal muscle.
  • Sequence similarities

    Belongs to the nuclear hormone receptor family. NR1 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • FAAR antibody
    • MGC3931 antibody
    • NR1C2 antibody
    • NUC1 antibody
    • NUCI antibody
    • NUCII antibody
    • Nuclear hormone receptor 1 antibody
    • Nuclear receptor subfamily 1 group C member 2 antibody
    • Peroxisome proliferative activated receptor delta antibody
    • Peroxisome proliferator-activated receptor beta (PPAR-beta) antibody
    • Peroxisome proliferator-activated receptor beta antibody
    • Peroxisome proliferator-activated receptor delta antibody
    • PPAR beta antibody
    • PPAR-beta antibody
    • PPAR-delta antibody
    • PPAR-ß antibody
    • PPARB antibody
    • ppard antibody
    • PPARD_HUMAN antibody
    see all

Images

  • Anti-PPAR delta antibody (ab21209) at 0.3 µg/ml + MOLT4 cell lysate in RIPA buffer at 35 µg

    Predicted band size: 50 kDa
    Observed band size: 55 kDa
    why is the actual band size different from the predicted?



    Primary incubation was 1 hour. Detected using chemiluminescence.

References

This product has been referenced in:

  • Blitek A & Szymanska M Regulation of expression and role of peroxisome proliferator-activated receptors (PPARs) in luminal epithelial and stromal cells of the porcine endometrium. Theriogenology 127:88-101 (2019). Read more (PubMed: 30677596) »
  • Borland MG  et al. Inhibition of tumorigenesis by peroxisome proliferator-activated receptor (PPAR)-dependent cell cycle blocks in human skin carcinoma cells. Toxicology 404-405:25-32 (2018). Read more (PubMed: 29729928) »
See all 8 Publications for this product

Customer reviews and Q&As

Filter by Application

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Application
Western blot
Sample
Mouse Cell lysate - whole cell (thymus)
Loading amount
20 µg
Specification
thymus
Gel Running Conditions
Reduced Denaturing (12 %)
Blocking step
BSA as blocking agent for 36 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 4°C

Abcam user community

Verified customer

Submitted Mar 17 2011

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