Western blot abreview for Anti-PPAR delta antibody

Below Average
Abreviews
Application
Western blot
Sample
Mouse Cell lysate - whole cell (thymus)
Loading amount
20 µg
Specification
thymus
Gel Running Conditions
Reduced Denaturing (12 %)
Blocking step
BSA as blocking agent for 36 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 4°C

Other product details

Dilution
1/500
Incubation time
36 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: blocking solution

Secondary antibody

Name
Non-Abcam antibody was used: donkey anti-goat IgG HRP
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL
Exposure
10 minute(s) and 0 second(s)
Bands
Specific: 52 kDa Non-specific: 70, 48, 45, kDa

Additional data

Abcam response
The submitted results suggest that ab21209 recognises specifically the Mouse PPAR delta. The non-specific bands may be avoided by optimizing the blocking step using 5% BSA for 2 hours at room temperature.

Abcam user community

Verified customer

Submitted Mar 17 2011

Sign up