Overview

  • Product name

  • Description

    Rabbit polyclonal to PPAR delta
  • Host species

    Rabbit
  • Specificity

    This affinity purified antibody is directed against mouse PPAR delta protein.
  • Tested applications

    Suitable for: ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide:

    MEQPQEETPEAREE

    , corresponding to N terminal amino acids 1-14 of Mouse PPAR delta .

  • Positive control

    • 3T3 cell lysate HepG2
  • General notes

    OMIM link - 600409

Properties

Applications

Our Abpromise guarantee covers the use of ab8937 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
WB 1/1000 - 1/4000. Detects a band of approximately 54 kDa (predicted molecular weight: 50 kDa).

Target

  • Function

    Ligand-activated transcription factor. Receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. Has a preference for poly-unsaturated fatty acids, such as gamma-linoleic acid and eicosapentanoic acid. Once activated by a ligand, the receptor binds to promoter elements of target genes. Regulates the peroxisomal beta-oxidation pathway of fatty acids. Functions as transcription activator for the acyl-CoA oxidase gene. Decreases expression of NPC1L1 once activated by a ligand.
  • Tissue specificity

    Ubiquitous with maximal levels in placenta and skeletal muscle.
  • Sequence similarities

    Belongs to the nuclear hormone receptor family. NR1 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • FAAR antibody
    • MGC3931 antibody
    • NR1C2 antibody
    • NUC1 antibody
    • NUCI antibody
    • NUCII antibody
    • Nuclear hormone receptor 1 antibody
    • Nuclear receptor subfamily 1 group C member 2 antibody
    • Peroxisome proliferative activated receptor delta antibody
    • Peroxisome proliferator-activated receptor beta (PPAR-beta) antibody
    • Peroxisome proliferator-activated receptor beta antibody
    • Peroxisome proliferator-activated receptor delta antibody
    • PPAR beta antibody
    • PPAR-beta antibody
    • PPAR-delta antibody
    • PPAR-ß antibody
    • PPARB antibody
    • ppard antibody
    • PPARD_HUMAN antibody
    see all

Images

  • ICC/IF image of ab8937 stained HepG2 cells (ab7900). The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum (ab7481) / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab8937, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Rabbit polyclonal to PPAR delta (ab8937).
    Each lane contains 20 ug 3T3 cell lysate

    Lane 1: ab8937 1/500
    Lane 2: ab8937 1/1000

    Secondary antibody: Goat anti-Rabbit (HRP) (ab6721) at 1/2000

    Rabbit polyclonal to PPAR delta (ab8937). Each lane contains 20 ug 3T3 cell lysate (ab7179) Lane 1: ab8937 1/500 Lane 2: ab8937 1/1000 Secondary antibody: Goat anti-Rabbit (HRP) (ab6721) at 1/2000

References

This product has been referenced in:

  • Mandai S  et al. WNK1 regulates skeletal muscle cell hypertrophy by modulating the nuclear localization and transcriptional activity of FOXO4. Sci Rep 8:9101 (2018). Read more (PubMed: 29904119) »
  • Mukherjee S  et al. Boosting efferocytosis in alveolar space using BCG vaccine to protect host against influenza pneumonia. PLoS One 12:e0180143 (2017). WB ; Mouse . Read more (PubMed: 28686604) »
See all 11 Publications for this product

Customer reviews and Q&As

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1-3 of 3 Abreviews

Application
Western blot
Sample
Human Cell lysate - whole cell (Colorectal cancer HCT116)
Loading amount
10 µg
Specification
Colorectal cancer HCT116
Gel Running Conditions
Non-reduced Denaturing (10% Gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Ms. Injoo Hwang

Verified customer

Submitted Nov 09 2009

Application
Western blot
Sample
Mouse Tissue lysate - nuclear (Liver)
Loading amount
20 µg
Specification
Liver
Gel Running Conditions
Reduced Denaturing (8%)
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Abcam user community

Verified customer

Submitted Jul 10 2009

Application
Western blot
Sample
Mouse Tissue lysate - whole (Brain)
Loading amount
30 µg
Specification
Brain
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Mar 07 2008

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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