Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR18517] to PPAR delta - BSA and Azide free
- Suitable for: WB, IP
- Reacts with: Mouse, Rat, Human
Product nameAnti-PPAR delta antibody [EPR18517] - BSA and Azide free
See all PPAR delta primary antibodies
DescriptionRabbit monoclonal [EPR18517] to PPAR delta - BSA and Azide free
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human PPAR delta aa 200-300. The exact sequence is proprietary.
Database link: Q03181
- WB: Human PPAR delta full length recombinant protein; Jurkat, K562, HeLa, C6 and NIH/3T3 whole cell lysates; Mouse brain, heart and spleen lysates; Rat brain, heart and kidney lysates. IP: NIH/3T3 whole cell lysate.
Ab223137 is the carrier-free version of ab178866. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab223137 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
Storage instructionsShipped at 4°C. Store at +4°C. Do Not Freeze.
Storage bufferConstituent: PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab223137 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Detects a band of approximately 52 kDa (predicted molecular weight: 50 kDa).|
|IP||Use at an assay dependent concentration.|
FunctionLigand-activated transcription factor. Receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. Has a preference for poly-unsaturated fatty acids, such as gamma-linoleic acid and eicosapentanoic acid. Once activated by a ligand, the receptor binds to promoter elements of target genes. Regulates the peroxisomal beta-oxidation pathway of fatty acids. Functions as transcription activator for the acyl-CoA oxidase gene. Decreases expression of NPC1L1 once activated by a ligand.
Tissue specificityUbiquitous with maximal levels in placenta and skeletal muscle.
Sequence similaritiesBelongs to the nuclear hormone receptor family. NR1 subfamily.
Contains 1 nuclear receptor DNA-binding domain.
- Information by UniProt
- FAAR antibody
- MGC3931 antibody
- NR1C2 antibody
PPAR delta was immunoprecipitated from 0.35 mg of NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate with ab178866 at 1/20 dilution. Western blot was performed from the immunoprecipitate using ab178866 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
Lane 1: NIH/3T3 whole cell lysate, 10µg (Input).
Lane 2: ab178866 IP in NIH/3T3 whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 30 seconds.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab178866).
ab223137 has been referenced in 1 publication.
- Wang L et al. Rosuvastatin relieves myocardial ischemia/reperfusion injury by upregulating PPAR-? and UCP2. Mol Med Rep 18:789-798 (2018). PubMed: 29845235