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RecombinantRabMAb

Recombinant Anti-PPAR delta antibody [EPR5185(2)] (ab173299)

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Western blot - Anti-PPAR delta antibody [EPR5185(2)] (ab173299)
  • Anti-PPAR delta antibody [EPR5185(2)] (ab173299)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5185(2)] to PPAR delta
  • Suitable for: WB
  • Reacts with: Human

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Recombinant Human PPAR delta protein (ab82051)

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Overview

  • Product name

    Anti-PPAR delta antibody [EPR5185(2)]
    See all PPAR delta primary antibodies
  • Description

    Rabbit monoclonal [EPR5185(2)] to PPAR delta
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human PPAR delta aa 150-250 (Cysteine residue). The exact sequence is proprietary.
    Database link: Q03181

  • Positive control

    • HCT-116, K562, and Jurkat cell lysates
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR5185(2)
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Zinc Finger
    • Cardiovascular
    • Heart
    • Cardiac metabolism
    • Kits/ Lysates/ Other
    • Kits
    • Cell Metabolism Kits
    • Lipid Metabolism Kits
    • Fatty acids
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Fatty acids
    • Metabolism
    • Types of disease
    • Obesity

Associated products

  • Alternative Versions

    • Anti-PPAR delta antibody [EPR5185(2)] - BSA and Azide free (ab249760)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • Jurkat whole cell lysate (ab30128)
    • Jurkat whole cell lysate (ab7899)
    • K562 whole cell lysate (ab7911)
  • Recombinant Protein

    • Recombinant Human PPAR delta protein (ab82051)
  • Related Products

    • Recombinant Human PPAR delta protein (ab81806)
    • Recombinant Human PPAR delta protein (ab82051)

Applications

Our Abpromise guarantee covers the use of ab173299 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Detects a band of approximately 52 kDa (predicted molecular weight: 50 kDa).
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
  • Target

    • Function

      Ligand-activated transcription factor. Receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. Has a preference for poly-unsaturated fatty acids, such as gamma-linoleic acid and eicosapentanoic acid. Once activated by a ligand, the receptor binds to promoter elements of target genes. Regulates the peroxisomal beta-oxidation pathway of fatty acids. Functions as transcription activator for the acyl-CoA oxidase gene. Decreases expression of NPC1L1 once activated by a ligand.
    • Tissue specificity

      Ubiquitous with maximal levels in placenta and skeletal muscle.
    • Sequence similarities

      Belongs to the nuclear hormone receptor family. NR1 subfamily.
      Contains 1 nuclear receptor DNA-binding domain.
    • Cellular localization

      Nucleus.
    • Target information above from: UniProt accession Q03181 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 5467 Human
      • Omim: 600409 Human
      • SwissProt: Q03181 Human
      • Unigene: 696032 Human
      • Alternative names

        • FAAR antibody
        • MGC3931 antibody
        • NR1C2 antibody
        • NUC1 antibody
        • NUCI antibody
        • NUCII antibody
        • Nuclear hormone receptor 1 antibody
        • Nuclear receptor subfamily 1 group C member 2 antibody
        • Peroxisome proliferative activated receptor delta antibody
        • Peroxisome proliferator-activated receptor beta (PPAR-beta) antibody
        • Peroxisome proliferator-activated receptor beta antibody
        • Peroxisome proliferator-activated receptor delta antibody
        • PPAR beta antibody
        • PPAR-beta antibody
        • PPAR-delta antibody
        • PPAR-ß antibody
        • PPARB antibody
        • ppard antibody
        • PPARD_HUMAN antibody
        see all

      Images

      • Western blot - Anti-PPAR delta antibody [EPR5185(2)] (ab173299)
        Western blot - Anti-PPAR delta antibody [EPR5185(2)] (ab173299)
        All lanes : Anti-PPAR delta antibody [EPR5185(2)] (ab173299) at 1/1000 dilution

        Lane 1 : HCT-116 cell lysate
        Lane 2 : K562 cell lysate
        Lane 3 : Jurkat cell lysate

        Lysates/proteins at 10 µg per lane.

        Predicted band size: 50 kDa
        Additional bands at: 52 kDa. We are unsure as to the identity of these extra bands.

      • Anti-PPAR delta antibody [EPR5185(2)] (ab173299)
        Anti-PPAR delta antibody [EPR5185(2)] (ab173299)

      Protocols

      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (0)

      Publishing research using ab173299? Please let us know so that we can cite the reference in this datasheet.

      ab173299 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

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      There are currently no Customer reviews or Questions for ab173299.
      Please use the links above to contact us or submit feedback about this product.

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