Recombinant Anti-PPAR delta antibody [EPR5185(2)] (ab173299)
![Western blot - Anti-PPAR delta antibody [EPR5185(2)] (ab173299)](https://www.abcam.com/ps/products/173/ab173299/Images/ab173299-196728-anti-ppar-delta-antibody-epr51852-western-blot.jpg "Western blot - Anti-PPAR delta antibody [EPR5185(2)] (ab173299)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5185(2)] to PPAR delta
- Suitable for: WB
- Reacts with: Human
Overview
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Product name
Anti-PPAR delta antibody [EPR5185(2)]
See all PPAR delta primary antibodies -
Description
Rabbit monoclonal [EPR5185(2)] to PPAR delta -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human PPAR delta aa 150-250 (Cysteine residue). The exact sequence is proprietary.
Database link: Q03181 -
Positive control
- HCT-116, K562, and Jurkat cell lysates
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR5185(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab173299 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/5000. Detects a band of approximately 52 kDa (predicted molecular weight: 50 kDa). |
Target
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Function
Ligand-activated transcription factor. Receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. Has a preference for poly-unsaturated fatty acids, such as gamma-linoleic acid and eicosapentanoic acid. Once activated by a ligand, the receptor binds to promoter elements of target genes. Regulates the peroxisomal beta-oxidation pathway of fatty acids. Functions as transcription activator for the acyl-CoA oxidase gene. Decreases expression of NPC1L1 once activated by a ligand. -
Tissue specificity
Ubiquitous with maximal levels in placenta and skeletal muscle. -
Sequence similarities
Belongs to the nuclear hormone receptor family. NR1 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 5467 Human
- Omim: 600409 Human
- SwissProt: Q03181 Human
- Unigene: 696032 Human
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Alternative names
- FAAR antibody
- MGC3931 antibody
- NR1C2 antibody
see all
Images
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Western blot - Anti-PPAR delta antibody [EPR5185(2)] (ab173299)All lanes : Anti-PPAR delta antibody [EPR5185(2)] (ab173299) at 1/1000 dilution
Lane 1 : HCT-116 cell lysate
Lane 2 : K562 cell lysate
Lane 3 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 50 kDa
Additional bands at: 52 kDa. We are unsure as to the identity of these extra bands. -
![Anti-PPAR delta antibody [EPR5185(2)] (ab173299)](https://www.abcam.com/ps/products/173/ab173299/Images/ab173299-2-benefits-of-recombinant-antibodies.png)
Anti-PPAR delta antibody [EPR5185(2)] (ab173299)
Datasheets and documents
References (0)
ab173299 has not yet been referenced specifically in any publications.
