Recombinant Anti-PPAR delta antibody [EPR5185(2)] (ab173299)
![Western blot - Anti-PPAR delta antibody [EPR5185(2)] (ab173299)](https://www.abcam.com/ps/products/173/ab173299/Images/ab173299-196728-anti-ppar-delta-antibody-epr51852-western-blot.jpg "Western blot - Anti-PPAR delta antibody [EPR5185(2)] (ab173299)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5185(2)] to PPAR delta
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-PPAR delta antibody [EPR5185(2)]
See all PPAR delta primary antibodies -
Description
Rabbit monoclonal [EPR5185(2)] to PPAR delta -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human PPAR delta aa 150-250 (Cysteine residue). The exact sequence is proprietary.
Database link: Q03181 -
Positive control
- HCT-116, K562, and Jurkat cell lysates
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR5185(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab173299 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/5000. Detects a band of approximately 52 kDa (predicted molecular weight: 50 kDa).
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| Notes |
|---|
|
WB
1/1000 - 1/5000. Detects a band of approximately 52 kDa (predicted molecular weight: 50 kDa). |
Target
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Function
Ligand-activated transcription factor. Receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. Has a preference for poly-unsaturated fatty acids, such as gamma-linoleic acid and eicosapentanoic acid. Once activated by a ligand, the receptor binds to promoter elements of target genes. Regulates the peroxisomal beta-oxidation pathway of fatty acids. Functions as transcription activator for the acyl-CoA oxidase gene. Decreases expression of NPC1L1 once activated by a ligand. -
Tissue specificity
Ubiquitous with maximal levels in placenta and skeletal muscle. -
Sequence similarities
Belongs to the nuclear hormone receptor family. NR1 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 5467 Human
- Omim: 600409 Human
- SwissProt: Q03181 Human
- Unigene: 696032 Human
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Alternative names
- FAAR antibody
- MGC3931 antibody
- NR1C2 antibody
see all
Images
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Western blot - Anti-PPAR delta antibody [EPR5185(2)] (ab173299)All lanes : Anti-PPAR delta antibody [EPR5185(2)] (ab173299) at 1/1000 dilution
Lane 1 : HCT-116 cell lysate
Lane 2 : K562 cell lysate
Lane 3 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 50 kDa
Additional bands at: 52 kDa. We are unsure as to the identity of these extra bands. -
![Anti-PPAR delta antibody [EPR5185(2)] (ab173299)](https://www.abcam.com/ps/products/173/ab173299/Images/ab173299-2-benefits-of-recombinant-antibodies.png)
Anti-PPAR delta antibody [EPR5185(2)] (ab173299)
Datasheets and documents
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Datasheet download
References (0)
ab173299 has not yet been referenced specifically in any publications.
