PPAR delta Transcription Factor Assay Kit (ab133106)

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Overview

  • Product name

    PPAR delta Transcription Factor Assay Kit

  • Detection method

    Colorimetric

  • Sample type

    Adherent cells, Suspension cells, Nuclear Extracts

  • Assay type

    Semi-quantitative

  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Mammals

  • Product overview

    Abcam's PPAR delta Transcription Factor Assay Kit (ab133106) is a non-radioactive, sensitive method for detecting specific transcription factor DNA binding activity in nuclear extracts.

    A 96-well enzyme-linked immunosorbent assay (ELISA) replaces the cumbersome radioactive electrophoretic mobility shift assay (EMSA). A specific double stranded DNA (dsDNA) sequence containing the peroxisome proliferator response element (PPRE) is immobilized onto the bottom of wells of a 96-well plate. PPARs contained in a nuclear extract, bind specifically to the PPRE. PPAR  delta is detected by addition of specific primary antibody directed against PPAR delta. A secondary antibody conjugated to HRP is added to provide a sensitive colorimetric readout at 450 nm. ab133106 detects human PPAR delta.

    This assay does not cross-react with PPAR gamma 1 and PPAR gamma 2 or PPAR alpha.

  • Notes

    Peroxisome proliferator-activated receptors (PPARs) are ligand activated nuclear receptors. Three PPAR subtypes have been identified: alpha, delta and gamma. PPARs can be activated by polyunsaturated fatty acids, eicosanoids and various synthtic ligands.

    PPAR delta is the least understood; however, identification of a high-affinity PPAR delta synthetic ligand suggests a potential role for this receptor in lipid metabolism and obesity. PPAR delta is ubiquitously expressed, but it is particularly abundant in tissues such as liver, intestine, kidney, abdominal adipose, and skeletal muscle.

  • Platform

    Microplate reader

Properties

Images

  • Functional Studies - PPAR delta Transcription Factor Assay Kit (ab133106)
    Functional Studies - PPAR delta Transcription Factor Assay Kit (ab133106)
    Panel A: Increasing amounts of positive control (total lysate) are assayed for PPAR delta DNA-binding activity using ab133106.
    Panel B: PPAR delta DNA-binding assays are performed in the presence of competitive dsDNA. The decrease in signal caused by addition of competitive dsDNA confirms the assay specificity.

Protocols

References

This product has been referenced in:

  • Sharif O  et al. The triggering receptor expressed on myeloid cells 2 inhibits complement component 1q effector mechanisms and exerts detrimental effects during pneumococcal pneumonia. PLoS Pathog 10:e1004167 (2014). Functional Studies ; Mouse . Read more (PubMed: 24945405) »
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