PPAR delta Transcription Factor Assay Kit (ab133106)

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Functional Studies - PPAR delta Transcription Factor Assay Kit (ab133106)

    Key features and details

    • Assay type: Semi-quantitative
    • Detection method: Colorimetric
    • Platform: Microplate reader
    • Sample type: Adherent cells, Nuclear Extracts, Suspension cells

    Overview

    • Product name

      PPAR delta Transcription Factor Assay Kit

    • Detection method

      Colorimetric

    • Sample type

      Adherent cells, Suspension cells, Nuclear Extracts

    • Assay type

      Semi-quantitative

    • Species reactivity

      Reacts with: Mouse, Human
      Predicted to work with: Mammals

    • Product overview

      Abcam's PPAR delta Transcription Factor Assay Kit (ab133106) is a non-radioactive, sensitive method for detecting specific transcription factor DNA binding activity in nuclear extracts.

      A 96-well enzyme-linked immunosorbent assay (ELISA) replaces the cumbersome radioactive electrophoretic mobility shift assay (EMSA). A specific double stranded DNA (dsDNA) sequence containing the peroxisome proliferator response element (PPRE) is immobilized onto the bottom of wells of a 96-well plate. PPARs contained in a nuclear extract, bind specifically to the PPRE. PPAR  delta is detected by addition of specific primary antibody directed against PPAR delta. A secondary antibody conjugated to HRP is added to provide a sensitive colorimetric readout at 450 nm. ab133106 detects human PPAR delta.

      This assay does not cross-react with PPAR gamma 1 and PPAR gamma 2 or PPAR alpha.

    • Notes

      Peroxisome proliferator-activated receptors (PPARs) are ligand activated nuclear receptors. Three PPAR subtypes have been identified: alpha, delta and gamma. PPARs can be activated by polyunsaturated fatty acids, eicosanoids and various synthtic ligands.

      PPAR delta is the least understood; however, identification of a high-affinity PPAR delta synthetic ligand suggests a potential role for this receptor in lipid metabolism and obesity. PPAR delta is ubiquitously expressed, but it is particularly abundant in tissues such as liver, intestine, kidney, abdominal adipose, and skeletal muscle.

    • Platform

      Microplate reader

    Properties

    Images

    • Functional Studies - PPAR delta Transcription Factor Assay Kit (ab133106)
      Functional Studies - PPAR delta Transcription Factor Assay Kit (ab133106)
      Panel A: Increasing amounts of positive control (total lysate) are assayed for PPAR delta DNA-binding activity using ab133106.
      Panel B: PPAR delta DNA-binding assays are performed in the presence of competitive dsDNA. The decrease in signal caused by addition of competitive dsDNA confirms the assay specificity.

    References (2)

    Publishing research using ab133106? Please let us know so that we can cite the reference in this datasheet.

    ab133106 has been referenced in 2 publications.

    • Huang KC  et al. Stearoyl-CoA Desaturase-1 Attenuates the High Shear Force Damage Effect on Human MG63 Osteosarcoma Cells. Int J Mol Sci 21:N/A (2020). PubMed: 32630668
    • Sharif O  et al. The triggering receptor expressed on myeloid cells 2 inhibits complement component 1q effector mechanisms and exerts detrimental effects during pneumococcal pneumonia. PLoS Pathog 10:e1004167 (2014). Functional Studies ; Mouse . PubMed: 24945405

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