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    ppar-delta-transcription-factor-assay-kit-ab133106.pdf

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Epigenetics and Nuclear Signaling Transcription Domain Families Zinc Finger
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PPAR delta Transcription Factor Assay Kit (ab133106)

  • Datasheet
  • SDS
  • Protocol Booklet
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Functional Studies - PPAR delta Transcription Factor Assay Kit (ab133106)

    Key features and details

    • Assay type: Semi-quantitative
    • Detection method: Colorimetric
    • Platform: Microplate reader
    • Sample type: Adherent cells, Nuclear Extracts, Suspension cells

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    Overview

    • Product name

      PPAR delta Transcription Factor Assay Kit
    • Detection method

      Colorimetric
    • Sample type

      Adherent cells, Suspension cells, Nuclear Extracts
    • Assay type

      Semi-quantitative
    • Species reactivity

      Reacts with: Mouse, Human
      Predicted to work with: Mammals
    • Product overview

      Abcam's PPAR delta Transcription Factor Assay Kit (ab133106) is a non-radioactive, sensitive method for detecting specific transcription factor DNA binding activity in nuclear extracts.

      A 96-well enzyme-linked immunosorbent assay (ELISA) replaces the cumbersome radioactive electrophoretic mobility shift assay (EMSA). A specific double stranded DNA (dsDNA) sequence containing the peroxisome proliferator response element (PPRE) is immobilized onto the bottom of wells of a 96-well plate. PPARs contained in a nuclear extract, bind specifically to the PPRE. PPAR  delta is detected by addition of specific primary antibody directed against PPAR delta. A secondary antibody conjugated to HRP is added to provide a sensitive colorimetric readout at 450 nm. ab133106 detects human PPAR delta.

      This assay does not cross-react with PPAR gamma 1 and PPAR gamma 2 or PPAR alpha.

    • Notes

      Peroxisome proliferator-activated receptors (PPARs) are ligand activated nuclear receptors. Three PPAR subtypes have been identified: alpha, delta and gamma. PPARs can be activated by polyunsaturated fatty acids, eicosanoids and various synthtic ligands.

      PPAR delta is the least understood; however, identification of a high-affinity PPAR delta synthetic ligand suggests a potential role for this receptor in lipid metabolism and obesity. PPAR delta is ubiquitously expressed, but it is particularly abundant in tissues such as liver, intestine, kidney, abdominal adipose, and skeletal muscle.

    • Platform

      Microplate reader

    Properties

    • Storage instructions

      Please refer to protocols.
    • Components 96 tests
      96-Well Plate Cover 1 unit
      Polysorbate 20 1 vial
      Transcription Factor Antibody Binding Buffer (10X) 1 x 3ml
      Transcription Factor Binding Assay Buffer (4X) 1 x 3ml
      Transcription Factor Developing Solution 1 x 12ml
      Transcription Factor Goat Anti-Rabbit HRP Conjugate 1 x 100µl
      Transcription Factor PPAR 96-Well Strip Plate 1 unit
      Transcription Factor PPAR Competitor dsDNA 1 vial
      Transcription Factor PPARδ Positive Control 1 vial
      Transcription Factor PPARδ Primary Antibody 1 vial
      Transcription Factor Reagent A 1 x 120µl
      Transcription Factor Stop Solution 1 x 12ml
      Wash Buffer Concentrate (400X) 1 x 5ml
    • Research areas

      • Epigenetics and Nuclear Signaling
      • Transcription
      • Domain Families
      • Zinc Finger
      • Cardiovascular
      • Heart
      • Cardiac metabolism
      • Kits/ Lysates/ Other
      • Kits
      • Cell Metabolism Kits
      • Lipid Metabolism Kits
      • Fatty acids
      • Kits/ Lysates/ Other
      • Kits
      • Cell Metabolism Kits
      • Lipid Metabolism Kits
      • Obesity
      • Metabolism
      • Pathways and Processes
      • Metabolic signaling pathways
      • Lipid and lipoprotein metabolism
      • Fatty acids
      • Metabolism
      • Types of disease
      • Obesity
      • Kits/ Lysates/ Other
      • Kits
      • Epigenetic kits
      • Transcription factor assay kits
      • Metabolism
      • Pathways and Processes
      • Redox metabolism
      • Fatty acid oxidation
    • Function

      Ligand-activated transcription factor. Receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. Has a preference for poly-unsaturated fatty acids, such as gamma-linoleic acid and eicosapentanoic acid. Once activated by a ligand, the receptor binds to promoter elements of target genes. Regulates the peroxisomal beta-oxidation pathway of fatty acids. Functions as transcription activator for the acyl-CoA oxidase gene. Decreases expression of NPC1L1 once activated by a ligand.
    • Tissue specificity

      Ubiquitous with maximal levels in placenta and skeletal muscle.
    • Sequence similarities

      Belongs to the nuclear hormone receptor family. NR1 subfamily.
      Contains 1 nuclear receptor DNA-binding domain.
    • Cellular localization

      Nucleus.
    • Target information above from: UniProt accession Q03181 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Alternative names

      • FAAR
      • MGC3931
      • NR1C2
      • NUC1
      • NUCI
      • NUCII
      • Nuclear hormone receptor 1
      • Nuclear receptor subfamily 1 group C member 2
      • Peroxisome proliferative activated receptor delta
      • Peroxisome proliferator-activated receptor beta
      • Peroxisome proliferator-activated receptor beta (PPAR-beta)
      • Peroxisome proliferator-activated receptor delta
      • PPAR beta
      • PPAR-beta
      • PPAR-delta
      • PPAR-ß
      • PPARB
      • ppard
      • PPARD_HUMAN
      see all
    • Database links

      • Entrez Gene: 5467 Human
      • Entrez Gene: 19015 Mouse
      • Omim: 600409 Human
      • SwissProt: Q03181 Human
      • SwissProt: P35396 Mouse
      • Unigene: 696032 Human
      • Unigene: 328914 Mouse

      Associated products

      • Related Products

        • Indomethacin, Non-selective COX inhibitor (ab120719)
        • Rosiglitazone, PPARgamma agonist (ab120762)
        • Pioglitazone hydrochloride, PPAR-gamma agonist (ab120794)
        • Telmisartan, angiotensin II (AT1) antagonist (ab120831)
        • Clofibric acid, PPARalpha agonist (ab120833)
        • GW 9662, PPARgamma antagonist (ab141125)
        • Ciglitazone, PPARgamma agonist (ab141139)
        • 15-Deoxy-Delta12,14-prostaglandin J2, Selective PPARgamma agonist (ab141717)
      • sELISA pair antibody

        • Nuclear Extraction Kit (ab113474)

      Images

      • Functional Studies - PPAR delta Transcription Factor Assay Kit (ab133106)
        Functional Studies - PPAR delta Transcription Factor Assay Kit (ab133106)
        Panel A: Increasing amounts of positive control (total lysate) are assayed for PPAR delta DNA-binding activity using ab133106.
        Panel B: PPAR delta DNA-binding assays are performed in the presence of competitive dsDNA. The decrease in signal caused by addition of competitive dsDNA confirms the assay specificity.

      Protocols

      • Protocol Booklet

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (3)

      Publishing research using ab133106? Please let us know so that we can cite the reference in this datasheet.

      ab133106 has been referenced in 3 publications.

      • Huang KC  et al. Stearoyl-CoA Desaturase-1 Attenuates the High Shear Force Damage Effect on Human MG63 Osteosarcoma Cells. Int J Mol Sci 21:N/A (2020). PubMed: 32630668
      • Watts AJ  et al. Regulation of Peroxisome Proliferator-Activated Receptor Pathway During Torpor in the Garden Dormouse, Eliomys quercinus. Front Physiol 11:615025 (2020). PubMed: 33408645
      • Sharif O  et al. The triggering receptor expressed on myeloid cells 2 inhibits complement component 1q effector mechanisms and exerts detrimental effects during pneumococcal pneumonia. PLoS Pathog 10:e1004167 (2014). Functional Studies ; Mouse . PubMed: 24945405

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