Product nameAnti-PPAR gamma 1+2 antibody [A3409A] - ChIP Grade
DescriptionMouse monoclonal [A3409A] to PPAR gamma 1+2 - ChIP Grade
SpecificityThis antibody specifically recognizes PPAR gamma 1 and 2. The specificity for each subtype regarding to alpha, gamma -1, -2 , delta was confirmed by each cDNA transfected to CHO cell and the lysate reacted with corresponding antibody in a panel; the antibody was found not to react with alpha or delta forms.
Tested applicationsSuitable for: IHC-P, WB, ELISA, IP, ChIPmore details
Species reactivityReacts with: Mouse, Rat, Human
Baculovirus-expressed recombinant fragment, corresponding to amino acids 3-105 of Human PPAR gamma 1+2.
- IHC: Rat adipose cells and rat placenta. WB: Nuclear lysate of 3T3l-1 cells induced for adipocyte differentiation
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.1% Sodium azide
Concentration information loading...
Purification notesPurified from ascites via ammonium sulfate fractionation.
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Fatty acids
ChIP Related Products
Our Abpromise guarantee covers the use of ab41928 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 10 µg/ml. Perform antigen retrieval by autoclave to preserve reproducibility.
Sections should be fixed with formalin for no longer than 48 hours.
|EMSA||Use at an assay dependent concentration.|
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 58 kDa. A nuclear extraction is recommended.|
|ELISA||Use a concentration of 0.012 µg/ml.|
|IP||Use at an assay dependent concentration.|
|ChIP||Use at an assay dependent concentration. PubMed: 16197558|
FunctionReceptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. Once activated by a ligand, the receptor binds to a promoter element in the gene for acyl-CoA oxidase and activates its transcription. It therefore controls the peroxisomal beta-oxidation pathway of fatty acids. Key regulator of adipocyte differentiation and glucose homeostasis.
Tissue specificityHighest expression in adipose tissue. Lower in skeletal muscle, spleen, heart and liver. Also detectable in placenta, lung and ovary.
Involvement in diseaseNote=Defects in PPARG can lead to type 2 insulin-resistant diabetes and hyptertension. PPARG mutations may be associated with colon cancer.
Defects in PPARG may be associated with susceptibility to obesity (OBESITY) [MIM:601665]. It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat.
Defects in PPARG are the cause of familial partial lipodystrophy type 3 (FPLD3) [MIM:604367]. Familial partial lipodystrophies (FPLD) are a heterogeneous group of genetic disorders characterized by marked loss of subcutaneous (sc) fat from the extremities. Affected individuals show an increased preponderance of insulin resistance, diabetes mellitus and dyslipidemia.
Genetic variations in PPARG can be associated with susceptibility to glioma type 1 (GLM1) [MIM:137800]. Gliomas are central nervous system neoplasms derived from glial cells and comprise astrocytomas, glioblastoma multiforme, oligodendrogliomas, and ependymomas. Note=Polymorphic PPARG alleles have been found to be significantly over-represented among a cohort of American patients with sporadic glioblastoma multiforme suggesting a possible contribution to disease susceptibility.
Sequence similaritiesBelongs to the nuclear hormone receptor family. NR1 subfamily.
Contains 1 nuclear receptor DNA-binding domain.
- Information by UniProt
- NR1C3 antibody
- Nuclear receptor subfamily 1 group C member 3 antibody
- Peroxisome proliferator-activated receptor gamma antibody
ab41928 staining PPAR gamma 1+2 in rat adiposoe cells (10 ug/mL) by immunohistochemistry, formalin-fixed paraffin embedded sections.
All lanes : Anti-PPAR gamma 1+2 antibody [A3409A] - ChIP Grade (ab41928) at 1 µg/ml
Lane 1 : Nuclear lysate of non induced
Lane 2 : Nuclear lysate of 3T3l-1 cells induced for adipocyte differentiation
Lysates/proteins at 20 µg per lane.
Predicted band size: 58 kDa
Two bands are visible: PPAR gamma 1 and PPAR gamma 2.
Lane 1: FLAG-M2
Lane 2: ab41928
Lane 3: ab41928
Lane 4: Control IgG
Immunoprecipitation analysis using Fluorescence labeled PPAR gamma generated in vitro translation. Anti-PPAR gamma antibody (ab41928) is diluted with translation production and mixed with protein G Sepharose beads. It is then rotated at 4oC for 1 hour and precipitates are eluted with addition of SDS loading buffer. Samples are incubated for 10minutes at 70°C and analyzed with Fluorescence imager.
ab41928 staining PPAR gamma 1+2 in rat plancenta(10 ug/mL) by immunohistochemistry, formalin-fixed paraffin embedded sections.
This product has been referenced in:
- Zhan Y et al. Aspirin-induced attenuation of adipogenic differentiation of bone marrow mesenchymal stem cells is accompanied by the disturbed epigenetic modification. Int J Biochem Cell Biol 98:29-42 (2018). WB, ICC/IF . Read more (PubMed: 29471042) »
- Heming M et al. Peroxisome Proliferator-Activated Receptor-? Modulates the Response of Macrophages to Lipopolysaccharide and Glucocorticoids. Front Immunol 9:893 (2018). Read more (PubMed: 29867927) »