Overview

  • Product name
    Anti-PPAR gamma 1+2 antibody [A3409A] - ChIP Grade
  • Description
    Mouse monoclonal [A3409A] to PPAR gamma 1+2 - ChIP Grade
  • Host species
    Mouse
  • Specificity
    This antibody specifically recognizes PPAR gamma 1 and 2. The specificity for each subtype regarding to alpha, gamma -1, -2 , delta was confirmed by each cDNA transfected to CHO cell and the lysate reacted with corresponding antibody in a panel; the antibody was found not to react with alpha or delta forms.
  • Tested applications
    Suitable for: IHC-P, WB, ELISA, IP, ChIPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Baculovirus-expressed recombinant fragment, corresponding to amino acids 3-105 of Human PPAR gamma 1+2.

  • Positive control
    • IHC: Rat adipose cells and rat placenta. WB: Nuclear lysate of 3T3l-1 cells induced for adipocyte differentiation

Properties

Applications

Our Abpromise guarantee covers the use of ab41928 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 10 µg/ml. Perform antigen retrieval by autoclave to preserve reproducibility.
Sections should be fixed with formalin for no longer than 48 hours.
EMSA Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 58 kDa. A nuclear extraction is recommended.
ELISA Use a concentration of 0.012 µg/ml.
IP Use at an assay dependent concentration.
ChIP Use at an assay dependent concentration. PubMed: 16197558

Target

  • Function
    Receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. Once activated by a ligand, the receptor binds to a promoter element in the gene for acyl-CoA oxidase and activates its transcription. It therefore controls the peroxisomal beta-oxidation pathway of fatty acids. Key regulator of adipocyte differentiation and glucose homeostasis.
  • Tissue specificity
    Highest expression in adipose tissue. Lower in skeletal muscle, spleen, heart and liver. Also detectable in placenta, lung and ovary.
  • Involvement in disease
    Note=Defects in PPARG can lead to type 2 insulin-resistant diabetes and hyptertension. PPARG mutations may be associated with colon cancer.
    Defects in PPARG may be associated with susceptibility to obesity (OBESITY) [MIM:601665]. It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat.
    Defects in PPARG are the cause of familial partial lipodystrophy type 3 (FPLD3) [MIM:604367]. Familial partial lipodystrophies (FPLD) are a heterogeneous group of genetic disorders characterized by marked loss of subcutaneous (sc) fat from the extremities. Affected individuals show an increased preponderance of insulin resistance, diabetes mellitus and dyslipidemia.
    Genetic variations in PPARG can be associated with susceptibility to glioma type 1 (GLM1) [MIM:137800]. Gliomas are central nervous system neoplasms derived from glial cells and comprise astrocytomas, glioblastoma multiforme, oligodendrogliomas, and ependymomas. Note=Polymorphic PPARG alleles have been found to be significantly over-represented among a cohort of American patients with sporadic glioblastoma multiforme suggesting a possible contribution to disease susceptibility.
  • Sequence similarities
    Belongs to the nuclear hormone receptor family. NR1 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • NR1C3 antibody
    • Nuclear receptor subfamily 1 group C member 3 antibody
    • Peroxisome proliferator-activated receptor gamma antibody
    • PPAR gamma antibody
    • PPAR-gamma antibody
    • PPARG antibody
    • PPARG_HUMAN antibody
    • PPARG1 antibody
    • PPARG2 antibody
    see all

Images

  • ab41928 staining PPAR gamma 1+2 in rat adiposoe cells (10 ug/mL) by immunohistochemistry, formalin-fixed paraffin embedded sections.
  • All lanes : Anti-PPAR gamma 1+2 antibody [A3409A] - ChIP Grade (ab41928) at 1 µg/ml

    Lane 1 : Nuclear lysate of non induced
    3T3l-1 cells
    Lane 2 : Nuclear lysate of 3T3l-1 cells induced for adipocyte differentiation

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 58 kDa



    Two bands are visible: PPAR gamma 1 and PPAR gamma 2.
  • Lane 1: FLAG-M2
    Lane 2: ab41928
    Lane 3: ab41928
    Lane 4: Control IgG

    Immunoprecipitation analysis using Fluorescence labeled PPAR gamma generated in vitro translation. Anti-PPAR gamma antibody (ab41928) is diluted with translation production and mixed with protein G Sepharose beads. It is then rotated at 4oC for 1 hour and precipitates are eluted with addition of SDS loading buffer. Samples are incubated for 10minutes at 70°C and analyzed with Fluorescence imager.

  • ab41928 staining PPAR gamma 1+2 in rat plancenta(10 ug/mL) by immunohistochemistry, formalin-fixed paraffin embedded sections.

References

This product has been referenced in:
  • Zhan Y  et al. Aspirin-induced attenuation of adipogenic differentiation of bone marrow mesenchymal stem cells is accompanied by the disturbed epigenetic modification. Int J Biochem Cell Biol 98:29-42 (2018). WB, ICC/IF . Read more (PubMed: 29471042) »
  • Heming M  et al. Peroxisome Proliferator-Activated Receptor-? Modulates the Response of Macrophages to Lipopolysaccharide and Glucocorticoids. Front Immunol 9:893 (2018). Read more (PubMed: 29867927) »
See all 21 Publications for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Tissue lysate - whole (fat)
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (gel 10%)
Loading amount
20 µg
Specification
fat
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 26°C

Abcam user community

Verified customer

Submitted Mar 14 2017

Application
Western blot
Sample
Mouse Tissue lysate - whole (liver)
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (10%gel)
Loading amount
15 µg
Specification
liver
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Aug 19 2015

Answer

Proteins that are found exclusively or predominantly in a sub-cellular location can be enriched in a lysate of the sub-cellular fraction compared to whole cell or tissue lysates. This can be useful when trying to obtain a signal for a weakly-expressed protein. For instance, a nuclear protein will be a larger proportion of the total protein in a nuclear lysate than it will be in a whole-cell or whole-tissue lysate, making it possible to load more of the protein per gel lane. Another advantage is the removal of potentially cross-reactive proteins present in the unused fractions.
For this particular antibody we recommend working with nuclear extracts to optimise the results. The buffer we would use for nuclear extraction is RIPA, but alternatively you could try the following protocol for nuclear fractionation: https://www.abcam.com/index.html?pageconfig=resource&rid=11408.
We also sell sine Cell fractionation kits, which prepare cytosolic, mitochondrial and nuclear fractions in microplate format (see ab109718).
I am afraid we don’t have any further information using this antibody with different conditions, but if you are unable to prepare nuclear lysates you could always try your cellular or tissue whole cell extracts and run a positive control in parallel.
The recommended concentration for using this antibody in WB is 10ug/ml, but it is always advisable to optimise the method as it varies enormously depending on the conditions and samples used.

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Answer

DISCOUNT CODE: xxxxxxxx
Expiration date: xxxxxxxxxx
Value: xxxxxxxxxxx
I am very pleased to hear you would like to accept our offer and test ab41928 in Flow Cytometry. This code will give you xxxxxxxxx off your next order before the expiration date. To redeem this offer, please submit an Abreview for Flow Cytometry and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.
Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.
Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.
The terms and conditions applicable to this offer can be found here: www.abcam.com/abtrial.

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Answer

Thank you for contacting us.
Regarding PPAR-delta ab:
I think ab58137 (www.abcam.com/ab58137) as you suggested would be the best antibody to try as it has been tested in flow cyt and using the same secondary ab (ab96879) would be fine. However you would have to use a mouse IgG1 as a control as the primary antibody (ab58137) has the isotype IgG1. I suggest isotype control antibody, mouse IgG1 [ICIGG1] ab91353 (www.abcam.com/ab91353).
Regarding PPAR-gamma ab, we do not have any antibodies which have been tested in flow cytometry.
However, if you would be willing to test one of these antibodies and providing us with your results, whether positive or negative, I could give you a discount.
Simply follow these easy steps below to apply for our AbTrial Program:
1. Reply to this email, letting us know you are interested in testing this product.
2. Our scientists will email you an inactive personal discount code for the value of the product.
3. Purchase and test the product at the regular price.
4. Submit your results, including your discount code in the additional notes section of your Abreview.
5. Once the Abreview is submitted, the discount code will become active.
6. Apply your discount code on your next order to receive that value off.
Please let me know if you have any questions about this offer and I would be happy to help you further.
The Terms and Conditions of this offer can be found at: www.abcam.com/abtrial.
If you wish to test the antibody you suggested, ab41928, you could use the same secondary antibody but you would need a mouse IgG2a as a control. I suggest ab91361 as an isotype control (www.abcam.com/ab91361).
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
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