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  1. Link

    ppar-gamma-antibody-ab59256.pdf

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Epigenetics and Nuclear Signaling Transcription Domain Families Zinc Finger
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Anti-PPAR gamma antibody (ab59256)

  • Datasheet
  • SDS
Reviews (2)Q&A (2)References (48)

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Western blot - Anti-PPAR gamma antibody (ab59256)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPAR gamma antibody (ab59256)
  • Immunocytochemistry/ Immunofluorescence - Anti-PPAR gamma antibody (ab59256)

Key features and details

  • Rabbit polyclonal to PPAR gamma
  • Suitable for: WB, ICC/IF, IHC-P
  • Reacts with: Human
  • Isotype: IgG

Get better batch-to-batch reproducibility with a recombinant antibody

Product image
Anti-PPAR gamma antibody [EPR18516] (ab178860)
  • Research with confidence – consistent and reproducible results with every batch
  • Long-term and scalable supply – powered by recombinant technology for fast production
  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    Anti-PPAR gamma antibody
    See all PPAR gamma primary antibodies
  • Description

    Rabbit polyclonal to PPAR gamma
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic non-phosphopeptide derived from human PPAR gamma around the phosphorylation site of serine 112 (P-A-SP-P-P).

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride

    Without Mg+2 and Ca+2
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab59256 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Zinc Finger
    • Neuroscience
    • Neurology process
    • Metabolism
    • Stem Cells
    • Mesenchymal Stem Cells
    • Adipogenesis
    • Cardiovascular
    • Atherosclerosis
    • Diabetes associated
    • Cardiovascular
    • Lipids / Lipoproteins
    • Fatty Acids
    • Metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Fatty acids
    • Metabolism
    • Types of disease
    • Diabetes
    • Metabolism
    • Types of disease
    • Obesity
    • Metabolism
    • Types of disease
    • Cancer
    • Metabolism
    • Types of disease
    • Heart disease
    • Metabolism
    • Pathways and Processes
    • Redox metabolism
    • Fatty acid oxidation

Associated products

  • Assay kits

    • PPAR gamma Transcription Factor Assay Kit (ab133101)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human PPAR gamma protein (ab53382)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab59256 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/500 - 1/1000. Predicted molecular weight: 57 kDa.
ICC/IF
Use a concentration of 5 µg/ml.
IHC-P (2)
1/50 - 1/100.
Notes
WB
1/500 - 1/1000. Predicted molecular weight: 57 kDa.
ICC/IF
Use a concentration of 5 µg/ml.
IHC-P
1/50 - 1/100.

Target

  • Function

    Receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. Once activated by a ligand, the receptor binds to a promoter element in the gene for acyl-CoA oxidase and activates its transcription. It therefore controls the peroxisomal beta-oxidation pathway of fatty acids. Key regulator of adipocyte differentiation and glucose homeostasis.
  • Tissue specificity

    Highest expression in adipose tissue. Lower in skeletal muscle, spleen, heart and liver. Also detectable in placenta, lung and ovary.
  • Involvement in disease

    Note=Defects in PPARG can lead to type 2 insulin-resistant diabetes and hyptertension. PPARG mutations may be associated with colon cancer.
    Defects in PPARG may be associated with susceptibility to obesity (OBESITY) [MIM:601665]. It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat.
    Defects in PPARG are the cause of familial partial lipodystrophy type 3 (FPLD3) [MIM:604367]. Familial partial lipodystrophies (FPLD) are a heterogeneous group of genetic disorders characterized by marked loss of subcutaneous (sc) fat from the extremities. Affected individuals show an increased preponderance of insulin resistance, diabetes mellitus and dyslipidemia.
    Genetic variations in PPARG can be associated with susceptibility to glioma type 1 (GLM1) [MIM:137800]. Gliomas are central nervous system neoplasms derived from glial cells and comprise astrocytomas, glioblastoma multiforme, oligodendrogliomas, and ependymomas. Note=Polymorphic PPARG alleles have been found to be significantly over-represented among a cohort of American patients with sporadic glioblastoma multiforme suggesting a possible contribution to disease susceptibility.
  • Sequence similarities

    Belongs to the nuclear hormone receptor family. NR1 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession P37231 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 5468 Human
    • Omim: 601487 Human
    • SwissProt: P37231 Human
    • Unigene: 162646 Human
    • Alternative names

      • CIMT1 antibody
      • GLM1 antibody
      • NR1C3 antibody
      • Nuclear receptor subfamily 1 group C member 3 antibody
      • OTTHUMP00000185032 antibody
      • OTTHUMP00000185036 antibody
      • Peroxisome proliferator activated nuclear receptor gamma variant 1 antibody
      • Peroxisome proliferator activated receptor gamma 1 antibody
      • Peroxisome Proliferator Activated Receptor gamma antibody
      • Peroxisome proliferator-activated receptor gamma antibody
      • PPAR gamma antibody
      • PPAR-gamma antibody
      • PPARG antibody
      • PPARG_HUMAN antibody
      • PPARG1 antibody
      • PPARG2 antibody
      • PPARgamma antibody
      see all

    Images

    • Western blot - Anti-PPAR gamma antibody (ab59256)
      Western blot - Anti-PPAR gamma antibody (ab59256)
      All lanes : Anti-PPAR gamma antibody (ab59256) at 1/500 dilution

      Lane 1 : HUVEC (human umbilical vein endothelial cell line) whole cell lysate
      Lane 2 : HUVEC (blocked with immunizing peptide), whole cell lysate

      Predicted band size: 57 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPAR gamma antibody (ab59256)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPAR gamma antibody (ab59256)
      ab59256, at 1/50 dilution, staining PPAR gamma in paraffin embedded human placenta tissue by Immunohistochemistry in the absence or presence of the immunising peptide.
    • Immunocytochemistry/ Immunofluorescence - Anti-PPAR gamma antibody (ab59256)
      Immunocytochemistry/ Immunofluorescence - Anti-PPAR gamma antibody (ab59256)
      ICC/IF image of ab59256 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab59256, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    Protocols

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (48)

    Publishing research using ab59256? Please let us know so that we can cite the reference in this datasheet.

    ab59256 has been referenced in 48 publications.

    • Hao Y  et al. TRIM27-mediated ubiquitination of PPAR? promotes glutamate-induced cell apoptosis and inflammation. Exp Cell Res 400:112437 (2021). PubMed: 33385414
    • Mouton AJ  et al. Interaction of Obesity and Hypertension on Cardiac Metabolic Remodeling and Survival Following Myocardial Infarction. J Am Heart Assoc 10:e018212 (2021). PubMed: 33666098
    • Luo P  et al. S-Allylmercaptocysteine improves alcoholic liver disease partly through a direct modulation of insulin receptor signaling. Acta Pharm Sin B 11:668-679 (2021). PubMed: 33777674
    • Chen L  et al. Injectable bio-responsive hydrogel for therapy of inflammation related eyelid diseases. Bioact Mater 6:3062-3073 (2021). PubMed: 33778188
    • Ansorge N  et al. Combined COX-2/PPAR? Expression as Independent Negative Prognosticator for Vulvar Cancer Patients. Diagnostics (Basel) 11:N/A (2021). PubMed: 33802010
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-4 of 4 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-PPAR gamma antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (placenta)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: EDTA-Buffer pH 9,0
    Permeabilization
    No
    Specification
    placenta
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Mar 14 2017

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-PPAR gamma antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (pancreas)
    Specification
    pancreas
    Fixative
    Formaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: TRIS-EDTA Buffer
    Permeabilization
    Yes - TRITON X
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    MR. Rudolf Jung

    Verified customer

    Submitted Feb 10 2009

    Question

    Thank you very much. I had checked the datasheet for ab59256 and ab19481. I realise that ab59256 is written aliquot and store at -20C, but for ab19481 it said store at 4C and do not freeze. Does it mean that ab59256 is better and will have a prolonged shelf life compared to ab19481? As storage at -20C is stable for 12 months it says, but for ab19481, no information is given regarding this...

    Many thanks!

    Best,

    Read More

    Abcam community

    Verified customer

    Asked on Jun 01 2012

    Answer

    Thank you for your response.

    Each antibody has specific storage buffer and recommended storage conditions. I would suggest checking this information carefully and follow the instructions. Generally, we suggest customers to use our antibodies within our guarantee period 6 months to make sure that our full Abpromise applies in case the product has been accidentally damaged or faulty.

    If you need any further assistance, please do not hesitate to contact me.

    Read More

    Abcam Scientific Support

    Answered on Jun 01 2012

    Question

    To provide more details, the cellsholocrine primarymeibomian epithelial cells from human eyelid, that when mature in vivo will burst to release their lipid content. Iculture the cellson collagen-coated cover slips and fixed them in 4% paraformaldehyde, 15min, room temp. The cells are cultured in CnT57 from cell n tec. P0 cells are obtained from organ culture of dissected gland, currently I can passage the cells up to passage 4 in the same media with no apparent loss of viability or change in morphology...



    Please let me know if more information is needed. Many thanks.

    Read More

    Abcam community

    Verified customer

    Asked on May 30 2012

    Answer

    Thank you for providing some further details of the cells/samples you wish to use in the experiments.

    ab59256 and ab19481 both have been tested for ICC/IF and they detect human protein so they would suit your need. However, since the cell types you wish to use is very specific and we do not have any data on them in terms of expression levels of PPAR gamma, I would suggest using positive control alongside with the samples to make sure that the experimental and staining conditions are fine and optimal.

    We have received good feedback on these antibodies from customers (published as Abreviews) and some specific publications are also available. Please take a look at the on-line datasheets to get some further information.

    BODIPY is a class of http://en.wikipedia.org/wiki/Fluorescence dyes and the signal can be bleached if you perform an additional staining for PPAR gamma with fluorochrome-labelled secondary antibodies.

    I would recommend performing the staining for PPAR gamma and lipid content separately. If you have good and acceptable results, then you may be able to combine the two staining procedures together but it may be necessary to optimize the double-staining steps.

    If you need any further assistance, please do not hesitate to contact me.

    Read More

    Abcam Scientific Support

    Answered on May 30 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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