Overview

  • Product name
    Anti-PPAR gamma antibody - ChIP Grade
    See all PPAR gamma primary antibodies
  • Description
    Rabbit polyclonal to PPAR gamma - ChIP Grade
  • Host species
    Rabbit
  • Specificity
    Ab45036 detects peroxisome proliferator activated receptor (PPAR) gamma 2. This antibody does not detect PPAR alpha or PPAR delta. This sequence is from P37231-1 (Isoform 2), the sequence is not present in P37231-2 (Isoform 1) or P37231-3 (Isoform 3).
  • Tested applications
    Suitable for: ChIP, IP, IHC-P, ICC/IF, WB, Functional Studies, EMSA, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Cow, Human, Non human primates
    Predicted to work with: Dog, Pig
  • Immunogen

    Synthetic peptide corresponding to Human PPAR gamma 2 aa 1-16.
    Sequence:

    MGETLGDSPIDPESDSC


    (Peptide available as ab41762)

  • Positive control
    • NIH-3T3 cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab45036 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ChIP Use at an assay dependent concentration. PubMed: 21247904
IP Use at an assay dependent concentration. PubMed: 21247904
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/500.
WB 1/500. Predicted molecular weight: 56 kDa.Can be blocked with Human PPAR gamma peptide (ab41762).

By Western blot, this antibody detects an ~56 kDa protein representing PPAR gamma 2 from NIH-3T3 cell lysate.

Functional Studies Use at an assay dependent concentration. Ab45036 inhibits PPAR gamma 2 DNA binding.
EMSA Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.

Target

  • Function
    Receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. Once activated by a ligand, the receptor binds to a promoter element in the gene for acyl-CoA oxidase and activates its transcription. It therefore controls the peroxisomal beta-oxidation pathway of fatty acids. Key regulator of adipocyte differentiation and glucose homeostasis.
  • Tissue specificity
    Highest expression in adipose tissue. Lower in skeletal muscle, spleen, heart and liver. Also detectable in placenta, lung and ovary.
  • Involvement in disease
    Note=Defects in PPARG can lead to type 2 insulin-resistant diabetes and hyptertension. PPARG mutations may be associated with colon cancer.
    Defects in PPARG may be associated with susceptibility to obesity (OBESITY) [MIM:601665]. It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat.
    Defects in PPARG are the cause of familial partial lipodystrophy type 3 (FPLD3) [MIM:604367]. Familial partial lipodystrophies (FPLD) are a heterogeneous group of genetic disorders characterized by marked loss of subcutaneous (sc) fat from the extremities. Affected individuals show an increased preponderance of insulin resistance, diabetes mellitus and dyslipidemia.
    Genetic variations in PPARG can be associated with susceptibility to glioma type 1 (GLM1) [MIM:137800]. Gliomas are central nervous system neoplasms derived from glial cells and comprise astrocytomas, glioblastoma multiforme, oligodendrogliomas, and ependymomas. Note=Polymorphic PPARG alleles have been found to be significantly over-represented among a cohort of American patients with sporadic glioblastoma multiforme suggesting a possible contribution to disease susceptibility.
  • Sequence similarities
    Belongs to the nuclear hormone receptor family. NR1 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • CIMT1 antibody
    • GLM1 antibody
    • NR1C3 antibody
    • Nuclear receptor subfamily 1 group C member 3 antibody
    • OTTHUMP00000185032 antibody
    • OTTHUMP00000185036 antibody
    • Peroxisome proliferator activated nuclear receptor gamma variant 1 antibody
    • Peroxisome proliferator activated receptor gamma 1 antibody
    • Peroxisome Proliferator Activated Receptor gamma antibody
    • Peroxisome proliferator-activated receptor gamma antibody
    • PPAR gamma antibody
    • PPAR-gamma antibody
    • PPARG antibody
    • PPARG_HUMAN antibody
    • PPARG1 antibody
    • PPARG2 antibody
    • PPARgamma antibody
    see all

Images

  • Lane 2 : Anti-PPAR gamma antibody - ChIP Grade (ab45036)

    Lane 1 : Protein Marker
    Lane 2 : Mouse liver tissue lysate at 1 µg

    Secondary
    Lane 2 : IRDye® 680RD Donkey anti-Rabbit

    Performed under reducing conditions.

    Predicted band size: 56 kDa


    Exposure time: 2 seconds

    See Abreview

  • ab45036 staining PPAR gamma in 3T3-L1 cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 3% BSA for 30 minutes at room temperature. Samples were incubated with primary antibody (1:200) for 1 hour at room temperature. A Dylight 680-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody (1/1000).

  • All lanes : Anti-PPAR gamma antibody - ChIP Grade (ab45036) at 1/500 dilution

    Lane 1 : 3T3
    Lane 2 : 3T3-L1 differentiated day 7

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 56 kDa



    PPAR gamma is detected in 3T3-L1 Day 7 differentiated cell lysates with some background bands. No detection of PPAR gamma occurs in 3T3 cell lysate.

     

  • ab45036 positive staining PPAR gamma in Hela cells by ICC/IF (Immunocytochemistry/immunofluorescence) (right) negative control in abscence of ab45036 (left). Cells were fixed with formalin, permeabilized with 0.1% Triton X-100 and blocked with 3% BSA for 30 minutes at room temperature. Samples were incubated with primary antibody (1/200 in PBS + 3% BSA) over night at 4°C. A DyLight 488-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody. F-actin (red) stained with red phalloidin and nuclei (blue) stainded with DAPI.

  • Immunocytochemical analysis of PPAR gamma using ab45036 at the dilution 1/200. The image at the top shows 3T3-L1 cells differntiated (for 7 days) where PPAR gamma is shown in green. The image below shows 3T3-L1 undifferentiated cells where no PPAR gamma is detected.

     

  • Immunocytochemical analysis of PPAR gamma using ab45036 at the dilution 1/200. 3T3-L1 cells providing positive signal have been differentiated for 7 days.

    PPAR gamma is shown in red, lipid droplets (that indicates the proper differentiation of the cells) are shown in green.

References

This product has been referenced in:
  • Wang J  et al. Ablation of protein phosphatase 5 (PP5) leads to enhanced both bone and cartilage development in mice. Cell Death Dis 9:214 (2018). Read more (PubMed: 29434189) »
  • Prabakaran AD  et al. Identification of early indicators of altered metabolism in normal development using a rodent model system. Dis Model Mech 11:N/A (2018). Read more (PubMed: 29434026) »
See all 33 Publications for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 22°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate
Sample
Human Tissue sections (Adipose)
Specification
Adipose
Permeabilization
No
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Jul 08 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (Liver)
Loading amount
1 µg
Specification
Liver
Gel Running Conditions
Reduced Denaturing
Blocking step
Odyssey Blocking Buffer as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 20°C

Dr. Xiaofeng Xin

Verified customer

Submitted May 13 2013

Answer

DISCOUNT CODE: 100%ABR-00000
Expiration date: 06. January 2013

I am very pleased to hear you would like to use ab45036 in ICC/IF and send us an image of your results.

This code will give youthe value of ab45036 off a future orderbefore the expiration date. To redeem this offer, please submit an Abreview with the image for ICC/IF and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

The code will be active once the Abreview with the image has been submitted and can be redeemed in one of the following ways:
1) Call to place your order and mention the code to our customer service department;
2) Include the code in your fax order;
3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated. Remember if the product does not work you will be covered by our Abpromise guarantee and eligible for a full refund or replacement.

If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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Answer

Thank you for contacting us. Because we carry over 94,000 products, it isn't feasible for us to keep small sample sizes of our products.

We are happy to reassure our customers that all of our products are covered by our Abpromise, which guarantees that the product will work in the applications and species specified on the datasheet, or we will offer a replacement, credit, or refund within 6 months of purchase.

ab45036 was not experimentally tested to not react withPPAR gamma 1. The immunogen sequence used to generate ab45036 does not exist in PPAR gamma 1, but only in PPAR gamma 2.

We do not currently have an image of ab45036 in immunofluorescence. Therefore, I can offer a discount off a future purchase if you buy ab45036 now, use it immunofluorescence and submit an image of your results to us in the form of an Abreview. The discount would be to the value of ab45036 .

It doesn’t matter whether the result is positive or negative, we would just really like to receive your feedback. Of course, if the results are negative you will be covered by our Abpromise guarantee and eligible for a refund or replacement.

If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed and send an image of ab45036 in immunofluorescence. I will then send a discount code. This code must be issued before purchasing ab45036 so please wait for my reply before ordering.

2. Purchase ab45036 either by phone, fax, or online (www.abcam.com).

3. Use it in immunofluorescence.

4. Send us an image of the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any product ordered and the discount code is valid for 4 months after issue.
Please remember that submission of the Abreview with image is sufficient for the discount code to become active.

We are always pleased to obtain feedback about our products and any information is greatly appreciated!

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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