Recombinant Anti-PPAR gamma antibody [EPR23297-111] (ab272718)
![Western blot - Anti-PPAR gamma antibody [EPR23297-111] (ab272718)](https://www.abcam.com/ps/products/272/ab272718/Images/ab272718-395302-anti-ppar-gamma-antibody-epr23297111-western-blot-rat-ovary-tissue-lysate-20-ug.jpg "Western blot - Anti-PPAR gamma antibody [EPR23297-111] (ab272718)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23297-111] to PPAR gamma
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-PPAR gamma antibody [EPR23297-111]
See all PPAR gamma primary antibodies -
Description
Rabbit monoclonal [EPR23297-111] to PPAR gamma -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-Fr,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human adipose and ovary tissue lysate; Rat ovary tissue lysate; Mouse ovary tissue lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23297-111 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab272718 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
1/1000. Detects a band of approximately 58 kDa (predicted molecular weight: 57 kDa).
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| Notes |
|---|
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WB
1/1000. Detects a band of approximately 58 kDa (predicted molecular weight: 57 kDa). |
Application notes
Is unsuitable for Flow Cyt,ICC/IF,IHC-Fr,IHC-P or IP.
Target
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Function
Receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. Once activated by a ligand, the receptor binds to a promoter element in the gene for acyl-CoA oxidase and activates its transcription. It therefore controls the peroxisomal beta-oxidation pathway of fatty acids. Key regulator of adipocyte differentiation and glucose homeostasis. -
Tissue specificity
Highest expression in adipose tissue. Lower in skeletal muscle, spleen, heart and liver. Also detectable in placenta, lung and ovary. -
Involvement in disease
Note=Defects in PPARG can lead to type 2 insulin-resistant diabetes and hyptertension. PPARG mutations may be associated with colon cancer.
Defects in PPARG may be associated with susceptibility to obesity (OBESITY) [MIM:601665]. It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat.
Defects in PPARG are the cause of familial partial lipodystrophy type 3 (FPLD3) [MIM:604367]. Familial partial lipodystrophies (FPLD) are a heterogeneous group of genetic disorders characterized by marked loss of subcutaneous (sc) fat from the extremities. Affected individuals show an increased preponderance of insulin resistance, diabetes mellitus and dyslipidemia.
Genetic variations in PPARG can be associated with susceptibility to glioma type 1 (GLM1) [MIM:137800]. Gliomas are central nervous system neoplasms derived from glial cells and comprise astrocytomas, glioblastoma multiforme, oligodendrogliomas, and ependymomas. Note=Polymorphic PPARG alleles have been found to be significantly over-represented among a cohort of American patients with sporadic glioblastoma multiforme suggesting a possible contribution to disease susceptibility. -
Sequence similarities
Belongs to the nuclear hormone receptor family. NR1 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 5468 Human
- Entrez Gene: 19016 Mouse
- Entrez Gene: 25664 Rat
- Omim: 601487 Human
- SwissProt: P37231 Human
- SwissProt: P37238 Mouse
- SwissProt: O88275 Rat
- Unigene: 162646 Human
see all -
Alternative names
- CIMT1 antibody
- GLM1 antibody
- NR1C3 antibody
see all
Images
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Western blot - Anti-PPAR gamma antibody [EPR23297-111] (ab272718)All lanes : Anti-PPAR gamma antibody [EPR23297-111] (ab272718) at 1/1000 dilution
Lane 1 : Rat ovary tissue lysate
Lane 2 : Mouse ovary tissue lysate
Lane 3 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/50000 dilution
Predicted band size: 57 kDa
Observed band size: 58 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Negative control: NIH/3T3 (PMID:12023027).
Exposure time: 3 minutes.
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![Western blot - Anti-PPAR gamma antibody [EPR23297-111] (ab272718)](https://www.abcam.com/ps/products/272/ab272718/Images/ab272718-395301-anti-ppar-gamma-antibody-epr23297111-western-blot-human-adipose-tissue-lysate-20-ug.jpg)
Western blot - Anti-PPAR gamma antibody [EPR23297-111] (ab272718)All lanes : Anti-PPAR gamma antibody [EPR23297-111] (ab272718) at 1/1000 dilution
Lane 1 : Human adipose tissue lysate
Lane 2 : Human ovary tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : VeriBlot for IP secondary antibody(HRP)(ab131366) at 1/1000 dilution
Predicted band size: 57 kDa
Observed band size: 58 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
This blot was developed using a higher sensitivity ECL substrate.
Exposure time: 59 seconds.
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![Anti-PPAR gamma antibody [EPR23297-111] (ab272718)](https://www.abcam.com/ps/products/272/ab272718/Images/ab272718-3-benefits-of-recombinant-antibodies.png)
Anti-PPAR gamma antibody [EPR23297-111] (ab272718)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (3)
ab272718 has been referenced in 3 publications.
- Zhao H et al. Propofol ameliorates endotoxin-induced myocardial cell injury by inhibiting inflammation and apoptosis via the PPAR?/HMGB1/NLRP3 axis. Mol Med Rep 23:N/A (2021). PubMed: 33398367
- Dai M et al. Icariin enhance mild hypothermia-induced neuroprotection via inhibiting the activation of NF-?B in experimental ischemic stroke. Metab Brain Dis N/A:N/A (2021). PubMed: 33978900
- Hu Y et al. Exosome-guided bone targeted delivery of Antagomir-188 as an anabolic therapy for bone loss. Bioact Mater 6:2905-2913 (2021). PubMed: 33718671
