Recombinant Anti-PPCEL/PREPL antibody [EPR17751] (ab202064)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR17751] to PPCEL/PREPL
- Suitable for: Flow Cyt (Intra), WB, ICC/IF, IP
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-PPCEL/PREPL antibody [EPR17751]
See all PPCEL/PREPL primary antibodies -
Description
Rabbit monoclonal [EPR17751] to PPCEL/PREPL -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, ICC/IF, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: MCF-7, NIH/3T3, A673 cell lysate, L6 cell lysate, Mouse brain lysate, Human fetal brain lysate, Human fetal heart lysate, Human fetal kidney lysate, C6 cell lysate, Raw264.7 cell lysate, PC12 cell lysate, Human PPCEL/PREPL full length protein; ICC/IF: SH-SY5Y and MCF-7 cells; Flow Cyt (intra): NIH/3T3 cells; IP: MCF-7 cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR17751 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab202064 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
1/150.
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WB |
1/1000. Detects a band of approximately 72 kDa (predicted molecular weight: 84 kDa).
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ICC/IF |
1/1000.
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IP |
1/40.
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Notes |
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Flow Cyt (Intra)
1/150. |
WB
1/1000. Detects a band of approximately 72 kDa (predicted molecular weight: 84 kDa). |
ICC/IF
1/1000. |
IP
1/40. |
Target
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Function
Probable serine peptidase whose precise substrate specificity remains unclear. Does not cleave peptides after a arginine or lysine residue. -
Tissue specificity
Widely expressed. Expressed at higher level in brain, skeletal muscle, heart and kidney. -
Involvement in disease
Defects in PREPL are a cause of hypotonia-cystinuria syndrome (HCS) [MIM:606407]. HCS is characterized generalized hypotonia at birth, nephrolithiasis, growth hormone deficiency, minor facial dysmorphism, failure to thrive, followed by hyperphagia and rapid weight gain in late childhood. HCS is caused by a deletion that disrups both SLC3A1 and PREPL genes. As SLC3A1 is known to cause isolated cystinuria type I, the extended phenotype could be attributed to the deletion of PREPL. -
Sequence similarities
Belongs to the peptidase S9A family. -
Cellular localization
Cytoplasm > cytosol. - Information by UniProt
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Database links
- Entrez Gene: 9581 Human
- Entrez Gene: 213760 Mouse
- Entrez Gene: 298771 Rat
- Omim: 609557 Human
- SwissProt: Q4J6C6 Human
- SwissProt: Q8C167 Mouse
- SwissProt: Q5HZA6 Rat
- Unigene: 444349 Human
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Alternative names
- PPCEL_HUMAN antibody
- prepl antibody
- Prolyl endopeptidase-like antibody
see all
Images
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Anti-PPCEL/PREPL antibody [EPR17751] (ab202064) at 1/10000 dilution + Recombinant Human PPCEL/PREPL protein (ab160557)
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 84 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?
Exposure time: 3 secondsBlocking and diluting buffer was 5% NFDM/TBS
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PPCEL/PREPL was immunoprecipitated from 1mg of MCF7 (Human breast adenocarcinoma cell line) whole cell extract with ab202064 at 1/40.
Western blot was performed using ab202064 at 1/2000.
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.
Lane 1: MCF7 whole cell lysate at 10ug.
Lane 2: Immunoprecipitate of MCF7 whole cell lysate using ab202064.
Lane 3: Immunoprecipitate of MCF7 whole cell lysate using ab172730 (Rabbit monoclonal IgG).
Blocking and dilution buffer and concentration: 5% NFDM/TBST. -
Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed NIH/3T3 (Mouse embryo fibroblast) cells labeling PPCEL/PREPL with ab202064 at 1/150 (red) compared with a rabbit monoclonal IgG isotype control (ab172730) (black) and a unlabelled control (cells without incubation with primary antibody and secondary antibody (blue)). Goat anti rabbit IgG (FITC) at 1/500 dilution was used as the secondary antibody.
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Immunofluorescent analysis of 100% methanol-fixed, 0.1% Triton X-100 permeabilized SH-SY5Y (Human neuroblastoma from bone marrow cells) cells labeling PPCEL/PREPL with ab202064 at 1/1000, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 (green). Confocal image showing cytoplasmic staining on SH-SY5Y cell line. The nuclear counterstain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) at 1/500 (red).
The negative controls are as follows:-
-ve control 1 - ab202064 at 1/1000 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) secondary antibody at 1/500 dilution.
-ve control 2. - Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution. -
Immunofluorescent analysis of 100% methanol-fixed, 0.1% Triton X-100 permeabilized MCF7 (Human breast adenocarcinoma cell line) cells labeling PPCEL/PREPL with ab202064 at 1/1000, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 (green). Confocal image showing cytoplasmic staining on MCF-7 cell line. The nuclear counterstain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) secondary antibody at 1/500 dilution (red).
The negative controls are as follows:-
-ve control 1: ab202064 at 1/1000 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) secondary at 1/500 dilution.
-ve control 2: Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary at 1/500 dilution. -
All lanes : Anti-PPCEL/PREPL antibody [EPR17751] (ab202064) at 1/1000 dilution
Lane 1 : C6 (Rat glial tumor cells) lysate
Lane 2 : RAW264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) lysate
Lane 3 : PC-12 (Rat adrenal gland pheochromocytoma) lysate
Lane 4 : NIH/3T3 (Mouse embryo fibroblast cells) lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 84 kDa
Observed band size: 72 kDa why is the actual band size different from the predicted?
Exposure time: 5 secondsBlocking and diluting buffer was 5% NFDM/TBS
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All lanes : Anti-PPCEL/PREPL antibody [EPR17751] (ab202064) at 1/1000 dilution
Lane 1 : Human fetal brain tissue lysate
Lane 2 : Human fetal heart tissue lysate
Lane 3 : Human fetal kidney tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 84 kDa
Observed band size: 72 kDa why is the actual band size different from the predicted?
Exposure time: 15 secondsBlocking and diluting buffer was 5% NFDM/TBS
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Anti-PPCEL/PREPL antibody [EPR17751] (ab202064) at 1/5000 dilution + Mouse brain lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 84 kDa
Observed band size: 72 kDa why is the actual band size different from the predicted?
Exposure time: 5 secondsBlocking and diluting buffer was 5% NFDM/TBS
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All lanes : Anti-PPCEL/PREPL antibody [EPR17751] (ab202064) at 1/5000 dilution
Lane 1 : A673 (Human muscle Ewing's Sarcoma cell line) lysate
Lane 2 : MCF7 (Human breast adenocarcinoma cell line ) lysate
Lane 3 : L6 (Rat skeletal muscle cell line) lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 84 kDa
Observed band size: 72 kDa why is the actual band size different from the predicted?
Exposure time: 15 secondsBlocking and diluting buffer was 5% NFDM/TBST
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab202064 has not yet been referenced specifically in any publications.