Overview

  • Product name

    Anti-PPCEL/PREPL antibody [EPR17751]
    See all PPCEL/PREPL primary antibodies
  • Description

    Rabbit monoclonal [EPR17751] to PPCEL/PREPL
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, IP, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human PPCEL/PREPL aa 50-150. The exact sequence is proprietary.
    Database link: Q4J6C6

  • Positive control

    • WB: MCF-7, NIH/3T3, A673 cell lysate, L6 cell lysate, Mouse brain lysate, Human fetal brain lysate, Human fetal heart lysate, Human fetal kidney lysate, C6 cell lysate, Raw264.7 cell lysate, PC12 cell lysate, Human PPCEL/PREPL full length protein; ICC/IF: SH-SY5Y and MCF-7 cells; Flow Cyt: NIH/3T3 cells; IP: MCF-7 cell lysate.
  • General notes

     

     

     This product was previously labelled as PPCEL

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR17751
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab202064 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 72 kDa (predicted molecular weight: 84 kDa).
ICC/IF 1/1000.
IP 1/40.
Flow Cyt 1/150.

Target

  • Function

    Probable serine peptidase whose precise substrate specificity remains unclear. Does not cleave peptides after a arginine or lysine residue.
  • Tissue specificity

    Widely expressed. Expressed at higher level in brain, skeletal muscle, heart and kidney.
  • Involvement in disease

    Defects in PREPL are a cause of hypotonia-cystinuria syndrome (HCS) [MIM:606407]. HCS is characterized generalized hypotonia at birth, nephrolithiasis, growth hormone deficiency, minor facial dysmorphism, failure to thrive, followed by hyperphagia and rapid weight gain in late childhood. HCS is caused by a deletion that disrups both SLC3A1 and PREPL genes. As SLC3A1 is known to cause isolated cystinuria type I, the extended phenotype could be attributed to the deletion of PREPL.
  • Sequence similarities

    Belongs to the peptidase S9A family.
  • Cellular localization

    Cytoplasm > cytosol.
  • Information by UniProt
  • Database links

  • Alternative names

    • PPCEL_HUMAN antibody
    • prepl antibody
    • Prolyl endopeptidase-like antibody
    • Prolylendopeptidase-like antibody
    • putative prolyl oligopeptidase antibody
    see all

Images

  • Anti-PPCEL/PREPL antibody [EPR17751] (ab202064) at 1/10000 dilution + Recombinant Human PPCEL/PREPL protein (ab160557)

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 84 kDa
    Observed band size: 110 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 seconds


    Blocking and diluting buffer was 5% NFDM/TBS

  • PPCEL/PREPL was immunoprecipitated from 1mg of MCF7 (Human breast adenocarcinoma cell line) whole cell extract with ab202064 at 1/40.

    Western blot was performed using ab202064 at 1/2000.

    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

    Lane 1: MCF7 whole cell lysate at 10ug.

    Lane 2: Immunoprecipitate of MCF7 whole cell lysate using ab202064.

    Lane 3: Immunoprecipitate of MCF7 whole cell lysate using ab172730 (Rabbit monoclonal IgG).
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

  • Flow cytometric analysis of 4% paraformaldehyde-fixed NIH/3T3 (Mouse embryo fibroblast) cells labeling PPCEL/PREPL with ab202064 at 1/150 (red) compared with a rabbit monoclonal IgG isotype control (ab172730) (black) and a unlabelled control (cells without incubation with primary antibody and secondary antibody (blue)). Goat anti rabbit IgG (FITC) at 1/500 dilution was used as the secondary antibody.

  • Immunofluorescent analysis of 100% methanol-fixed, 0.1% Triton X-100 permeabilized SH-SY5Y (Human neuroblastoma from bone marrow cells) cells labeling PPCEL/PREPL with ab202064 at 1/1000, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 (green). Confocal image showing cytoplasmic staining on SH-SY5Y cell line. The nuclear counterstain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) at 1/500 (red).
    The negative controls are as follows:-
    -ve control 1 - ab202064 at 1/1000 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) secondary antibody at 1/500 dilution.
    -ve control 2. - Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution.

  • Immunofluorescent analysis of 100% methanol-fixed, 0.1% Triton X-100 permeabilized MCF7 (Human breast adenocarcinoma cell line) cells labeling PPCEL/PREPL with ab202064 at 1/1000, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 (green). Confocal image showing cytoplasmic staining on MCF-7 cell line. The nuclear counterstain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) secondary antibody at 1/500 dilution (red).

    The negative controls are as follows:-

    -ve control 1: ab202064 at 1/1000 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) secondary at 1/500 dilution.
    -ve control 2: Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary at 1/500 dilution.

  • All lanes : Anti-PPCEL/PREPL antibody [EPR17751] (ab202064) at 1/1000 dilution

    Lane 1 : C6 (Rat glial tumor cells) lysate
    Lane 2 : Raw264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus)) lysate
    Lane 3 : PC-12 (Rat adrenal gland pheochromocytoma) lysate
    Lane 4 : NIH/3T3 (Mouse embryo fibroblast cells) lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 84 kDa
    Observed band size: 72 kDa why is the actual band size different from the predicted?


    Exposure time: 5 seconds


    Blocking and diluting buffer was 5% NFDM/TBS

  • All lanes : Anti-PPCEL/PREPL antibody [EPR17751] (ab202064) at 1/1000 dilution

    Lane 1 : Human fetal brain lysate
    Lane 2 : Human fetal heart lysate
    Lane 3 : Human fetal kidney lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 84 kDa
    Observed band size: 72 kDa why is the actual band size different from the predicted?


    Exposure time: 15 seconds


    Blocking and diluting buffer was 5% NFDM/TBS

  • Anti-PPCEL/PREPL antibody [EPR17751] (ab202064) at 1/5000 dilution + Mouse brain lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 84 kDa
    Observed band size: 72 kDa why is the actual band size different from the predicted?


    Exposure time: 5 seconds


    Blocking and diluting buffer was 5% NFDM/TBS

  • All lanes : Anti-PPCEL/PREPL antibody [EPR17751] (ab202064) at 1/5000 dilution

    Lane 1 : A673 (Human muscle Ewing's Sarcoma cell line) lysate
    Lane 2 : MCF7 (Human breast adenocarcinoma cell line ) lysate
    Lane 3 : L6 (Rat skeletal muscle cell line) lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 84 kDa
    Observed band size: 72 kDa why is the actual band size different from the predicted?


    Exposure time: 15 seconds


    Blocking and diluting buffer was 5% NFDM/TBST

References

ab202064 has not yet been referenced specifically in any publications.

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