Recombinant Anti-PPCEL/PREPL antibody [EPR17752] (ab203111)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR17752] to PPCEL/PREPL
- Suitable for: WB, IHC-P, ICC/IF, IP
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-PPCEL/PREPL antibody [EPR17752]
See all PPCEL/PREPL primary antibodies -
Description
Rabbit monoclonal [EPR17752] to PPCEL/PREPL -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IF, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment within Human PPCEL/PREPL aa 600-700. The exact sequence is proprietary.
Database link: Q4J6C6 -
Positive control
- WB: Human fetal brain, MCF-7, Mouse brain, Rat brain, C6, Raw264.7, PC-12 or NIH/3T3 whole cell lysate. IHC-P: Human, Mouse or Rat cerebral cortex tissue. ICC/IF: SH-SY5Y or MCF-7 cells. Flow Cyt: MCF-7 cells.
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General notes
This product was previously labelled as PPCEL
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR17752 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab203111 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | 1/2000. Detects a band of approximately 72 kDa (predicted molecular weight: 84 kDa). | |
IHC-P | 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. | |
ICC/IF | 1/250. | |
IP | 1/30. |
Target
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Function
Probable serine peptidase whose precise substrate specificity remains unclear. Does not cleave peptides after a arginine or lysine residue. -
Tissue specificity
Widely expressed. Expressed at higher level in brain, skeletal muscle, heart and kidney. -
Involvement in disease
Defects in PREPL are a cause of hypotonia-cystinuria syndrome (HCS) [MIM:606407]. HCS is characterized generalized hypotonia at birth, nephrolithiasis, growth hormone deficiency, minor facial dysmorphism, failure to thrive, followed by hyperphagia and rapid weight gain in late childhood. HCS is caused by a deletion that disrups both SLC3A1 and PREPL genes. As SLC3A1 is known to cause isolated cystinuria type I, the extended phenotype could be attributed to the deletion of PREPL. -
Sequence similarities
Belongs to the peptidase S9A family. -
Cellular localization
Cytoplasm > cytosol. - Information by UniProt
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Database links
- Entrez Gene: 9581 Human
- Entrez Gene: 213760 Mouse
- Entrez Gene: 298771 Rat
- Omim: 609557 Human
- SwissProt: Q4J6C6 Human
- SwissProt: Q8C167 Mouse
- SwissProt: Q5HZA6 Rat
- Unigene: 444349 Human
see all -
Alternative names
- PPCEL_HUMAN antibody
- prepl antibody
- Prolyl endopeptidase-like antibody
see all
Images
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Lane 1 : Anti-PPCEL/PREPL antibody [EPR17752] (ab203111) at 1/1000 dilution
Lane 2 : Anti-GST antibody [EPR4236] (ab111947) at 1/10000 dilution
All lanes : Recombinant Human PPCEL/PREPL protein (ab160557)
Lysates/proteins at 0.01 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 84 kDa
Observed band size: 109 kDa why is the actual band size different from the predicted?Exposure time:
Lane 1: 3 seconds.
Lane 2: 3 minutes.
Blocking and dilution buffer: 5% NFDM/TBST.
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Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized SH-SY5Y (Human neuroblastoma from bone marrow cells) cells labeling PPCEL/PREPL with ab203111 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on SH-SY5Y cell line. The nuclear counterstain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).
The negative controls are as follows:-
-ve control 1 - ab203111 at 1/250 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
-ve control 2. - ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution. -
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF-7(Human breast adenocarcinoma cell line) cells labeling PPCEL/PREPL with ab203111 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on MCF-7 cell line. The nuclear counterstain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).
The negative controls are as follows:-
-ve control 1 - ab203111 at 1/250 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
-ve control 2. - ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution. -
All lanes : Anti-PPCEL/PREPL antibody [EPR17752] (ab203111) at 1/2000 dilution
Lane 1 : Human fetal brain lysate
Lane 2 : MCF-7 (Human breast adenocarcinoma cell line) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution
Predicted band size: 84 kDa
Observed band size: 72 kDa why is the actual band size different from the predicted?
Exposure time: 1 minuteBlocking/Dilution buffer 5% NFDM/TBST.
The observed MW is consistent with what has been described in the literature PMID: 16385448.
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All lanes : Anti-PPCEL/PREPL antibody [EPR17752] (ab203111) at 1/2000 dilution
Lane 1 : Mouse brain whole cell lysate
Lane 2 : Rat brain whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 84 kDa
Observed band size: 72 kDa why is the actual band size different from the predicted?
Exposure time: 15 secondsBlocking/DIlution buffer 5% NFDM/TBST.
The observed MW is consistent with what has been described in the literature PMID: 16385448.
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All lanes : Anti-PPCEL/PREPL antibody [EPR17752] (ab203111) at 1/2000 dilution
Lane 1 : C6 (Rat glial tumor cells) whole cell lysate
Lane 2 : Raw264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus whole cell lysate
Lane 3 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate
Lane 4 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 84 kDa
Observed band size: 72 kDa why is the actual band size different from the predicted?
Exposure time: 30 secondsBlocking/Dilution buffer 5% NFDM/TBST.
The observed MW is consistent with what has been described in the literature PMID: 16385448.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPCEL/PREPL antibody [EPR17752] (ab203111)
Immunohistochemical analysis of paraffin-embedded Human cerebral cortex tissue labeling PPCEL/PREPL with ab203111 at 1/250 dilution followed by ab97051 Goat Anti-Rabbit IgG H&L (HRP) at a 1/500 dilution. Cytoplasmic staining on Human cerebral cortex tissue is observed. Counter stained with Hematoxylin.
Negative control: Used PBS instead of primary antibody, secondary antibody is ab97051 Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPCEL/PREPL antibody [EPR17752] (ab203111)
Immunohistochemical analysis of paraffin-embedded Mouse cerebral cortex tissue labeling PPCEL/PREPL with ab203111 at 1/250 dilution followed by ab97051 Goat Anti-Rabbit IgG H&L (HRP) at a 1/500 dilution. Cytoplasmic staining on Mouse cerebral cortex tissue is observed. Counter stained with Hematoxylin.
Negative control: Used PBS instead of primary antibody, secondary antibody is ab97051 Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPCEL/PREPL antibody [EPR17752] (ab203111)
Immunohistochemical analysis of paraffin-embedded Rat cerebral cortex tissue labeling PPCEL/PREPL with ab203111 at 1/250 dilution followed by ab97051 Goat Anti-Rabbit IgG H&L (HRP) at a 1/500 dilution. Cytoplasmic staining on Rat cerebral cortex tissue is observed. Counter stained with Hematoxylin.
Negative control: Used PBS instead of primary antibody, secondary antibody is ab97051 Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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PPCEL/PREPL was immunoprecipitated from 1mg of NIH/3T3 (Mouse embyro fibroblast cells) whole cell extract with ab203111 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab203111 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.
Lane 1: NIH/3T3 whole cell extract 10 µg (Input). Lane 2: ab203111 IP in NIH/3T3 whole cell extract. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab203111 in NIH/3T3 whole cell extract.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.Exposure time: 10 seconds
Blocking/Dilution Buffer: 5% NFDM/TBST
Protocols
Datasheets and documents
Certificate of Compliance
References (0)
ab203111 has not yet been referenced specifically in any publications.