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Signal Transduction Metabolism Amino Acids
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RecombinantRabMAb

Recombinant Anti-PPCS antibody [EPR9453] (ab140626)

  • Datasheet
  • SDS
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Western blot - Anti-PPCS antibody [EPR9453] (ab140626)
  • Anti-PPCS antibody [EPR9453] (ab140626)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9453] to PPCS
  • Suitable for: WB
  • Reacts with: Human

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Overview

  • Product name

    Anti-PPCS antibody [EPR9453]
    See all PPCS primary antibodies
  • Description

    Rabbit monoclonal [EPR9453] to PPCS
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC or IHC-P
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human PPCS aa 250-350 (C terminal). The exact sequence is proprietary.

  • Positive control

    • HeLa, 293T, SH-SY5Y and HepG2 whole cell lysate (ab7900).
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20ºC.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR9453
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Metabolism
    • Amino Acids
    • Signal Transduction
    • Metabolism
    • Vitamins / Minerals
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Amino acid metabolism
    • Metabolism
    • Pathways and Processes
    • Cofactors, Vitamins / minerals
    • Vitamins / minerals

Associated products

  • Alternative Versions

    • Anti-PPCS antibody [EPR9453] - BSA and Azide free (ab175048)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • HeLa whole cell lysate (ab29545)
  • Recombinant Protein

    • Recombinant Human PPCS protein (ab107145)
  • Related Products

    • Recombinant Human PPCS protein (ab107145)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab140626 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 34 kDa.
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 34 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IHC-P.
  • Target

    • Function

      Catalyzes the first step in the biosynthesis of coenzyme A from vitamin B5, where cysteine is conjugated to 4'-phosphopantothenate to form 4-phosphopantothenoylcysteine.
    • Pathway

      Cofactor biosynthesis; coenzyme A biosynthesis; CoA from (R)-pantothenate: step 2/5.
    • Sequence similarities

      Belongs to the PPC synthetase family.
    • Target information above from: UniProt accession Q9HAB8 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 79717 Human
      • Omim: 609853 Human
      • SwissProt: Q9HAB8 Human
      • Unigene: 706662 Human
      • Alternative names

        • COAB antibody
        • FLJ11838 antibody
        • MGC117357 antibody
        • MGC138220 antibody
        • OTTHUMP00000008433 antibody
        • Phosphopantothenate--cysteine ligase antibody
        • Phosphopantothenoylcysteine synthetase antibody
        • PPC synthetase antibody
        • Ppcs antibody
        • PPCS_HUMAN antibody
        • RP11-163G10.1 antibody
        see all

      Images

      • Western blot - Anti-PPCS antibody [EPR9453] (ab140626)
        Western blot - Anti-PPCS antibody [EPR9453] (ab140626)
        All lanes : Anti-PPCS antibody [EPR9453] (ab140626) at 1/1000 dilution

        Lane 1 : HeLa cell lysate
        Lane 2 : 293T cell lysate
        Lane 3 : SH-SY5Y cell lysate
        Lane 4 : HepG2 cell lysate

        Lysates/proteins at 10 µg per lane.

        Predicted band size: 34 kDa

      • Anti-PPCS antibody [EPR9453] (ab140626)
        Anti-PPCS antibody [EPR9453] (ab140626)

      Protocols

      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (1)

      Publishing research using ab140626? Please let us know so that we can cite the reference in this datasheet.

      ab140626 has been referenced in 1 publication.

      • Iuso A  et al. Mutations in PPCS, Encoding Phosphopantothenoylcysteine Synthetase, Cause Autosomal-Recessive Dilated Cardiomyopathy. Am J Hum Genet 102:1018-1030 (2018). PubMed: 29754768

      Customer reviews and Q&As

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