Recombinant
RabMAb

Recombinant Anti-PPCS antibody [EPR9453] (ab140626)

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Overview

  • Product name

    Anti-PPCS antibody [EPR9453]
    See all PPCS primary antibodies

  • Description

    Rabbit monoclonal [EPR9453] to PPCS

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC or IHC-P

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human PPCS aa 250-350 (C terminal). The exact sequence is proprietary.

  • Positive control

    • HeLa, 293T, SH-SY5Y and HepG2 whole cell lysate (ab7900).

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab140626 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 34 kDa.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IHC-P.

    • Target

    • Function

      Catalyzes the first step in the biosynthesis of coenzyme A from vitamin B5, where cysteine is conjugated to 4'-phosphopantothenate to form 4-phosphopantothenoylcysteine.

    • Pathway

      Cofactor biosynthesis; coenzyme A biosynthesis; CoA from (R)-pantothenate: step 2/5.

    • Sequence similarities

      Belongs to the PPC synthetase family.
    • Information by UniProt

    • Database links

      • Alternative names

        • COAB antibody
        • FLJ11838 antibody
        • MGC117357 antibody
        • MGC138220 antibody
        • OTTHUMP00000008433 antibody
        • Phosphopantothenate--cysteine ligase antibody
        • Phosphopantothenoylcysteine synthetase antibody
        • PPC synthetase antibody
        • Ppcs antibody
        • PPCS_HUMAN antibody
        • RP11-163G10.1 antibody
        see all

      Images

      • Western blot - Anti-PPCS antibody [EPR9453] (ab140626)
        Western blot - Anti-PPCS antibody [EPR9453] (ab140626)
        All lanes : Anti-PPCS antibody [EPR9453] (ab140626) at 1/1000 dilution

        Lane 1 : HeLa cell lysate
        Lane 2 : 293T cell lysate
        Lane 3 : SH-SY5Y cell lysate
        Lane 4 : HepG2 cell lysate

        Lysates/proteins at 10 µg per lane.

        Predicted band size: 34 kDa

      References

      This product has been referenced in:

      • Iuso A  et al. Mutations in PPCS, Encoding Phosphopantothenoylcysteine Synthetase, Cause Autosomal-Recessive Dilated Cardiomyopathy. Am J Hum Genet 102:1018-1030 (2018). Read more (PubMed: 29754768) »
      See 1 Publication for this product

      Publishing research using ab140626? Please let us know so that we can cite the reference in this datasheet.

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