Recombinant Anti-PPCS antibody [EPR9453] (ab140626)
![Western blot - Anti-PPCS antibody [EPR9453] (ab140626)](https://www.abcam.com/ps/products/140/ab140626/Images/ab140626-155921-anti-ppcs-antibody-epr9453-western-blot.jpg "Western blot - Anti-PPCS antibody [EPR9453] (ab140626)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9453] to PPCS
- Suitable for: WB
- Reacts with: Human
Overview
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Product name
Anti-PPCS antibody [EPR9453]
See all PPCS primary antibodies -
Description
Rabbit monoclonal [EPR9453] to PPCS -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC or IHC-P -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human PPCS aa 250-350 (C terminal). The exact sequence is proprietary.
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Positive control
- HeLa, 293T, SH-SY5Y and HepG2 whole cell lysate (ab7900).
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR9453 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab140626 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 34 kDa.
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| Notes |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 34 kDa. |
Target
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Function
Catalyzes the first step in the biosynthesis of coenzyme A from vitamin B5, where cysteine is conjugated to 4'-phosphopantothenate to form 4-phosphopantothenoylcysteine. -
Pathway
Cofactor biosynthesis; coenzyme A biosynthesis; CoA from (R)-pantothenate: step 2/5. -
Sequence similarities
Belongs to the PPC synthetase family. - Information by UniProt
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Database links
- Entrez Gene: 79717 Human
- Omim: 609853 Human
- SwissProt: Q9HAB8 Human
- Unigene: 706662 Human
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Alternative names
- COAB antibody
- FLJ11838 antibody
- MGC117357 antibody
see all
Images
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Western blot - Anti-PPCS antibody [EPR9453] (ab140626)All lanes : Anti-PPCS antibody [EPR9453] (ab140626) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : 293T cell lysate
Lane 3 : SH-SY5Y cell lysate
Lane 4 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 34 kDa -
![Anti-PPCS antibody [EPR9453] (ab140626)](https://www.abcam.com/ps/products/140/ab140626/Images/ab140626-2-benefits-of-recombinant-antibodies.png)
Anti-PPCS antibody [EPR9453] (ab140626)
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab140626 has been referenced in 1 publication.
- Iuso A et al. Mutations in PPCS, Encoding Phosphopantothenoylcysteine Synthetase, Cause Autosomal-Recessive Dilated Cardiomyopathy. Am J Hum Genet 102:1018-1030 (2018). PubMed: 29754768