Key features and details
- Rabbit polyclonal to PPFIA1
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-PPFIA1 antibody
See all PPFIA1 primary antibodies
DescriptionRabbit polyclonal to PPFIA1
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
- Human testis tissue, RT-4 and U-251 lysates, U-2 OS cells.
Previously labelled as Liprin alpha 1.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab204406 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/2500 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/100 - 1/250. Predicted molecular weight: 136 kDa.|
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.|
FunctionMay regulate the disassembly of focal adhesions. May localize receptor-like tyrosine phosphatases type 2A at specific sites on the plasma membrane, possibly regulating their interaction with the extracellular environment and their association with substrates.
Sequence similaritiesBelongs to the liprin family. Liprin-alpha subfamily.
Contains 3 SAM (sterile alpha motif) domains.
DomainThe N-terminal coiled coil regions mediate homodimerization preferentially and heterodimerization type alpha/alpha. The C-terminal, non-coiled coil regions mediate heterodimerization type alpha/beta and interaction with PTPRD, PTPRF and PTPRS.
Cellular localizationCytoplasm. Colocalizes with PTPRF at the ends of focal adhesions most proximal to the cell nucleus.
- Information by UniProt
- FLJ42630 antibody
- HGNC:9245 antibody
- LAR interacting protein 1 antibody
All lanes : Anti-ATP9A antibody (ab204404) at 1/100 dilution
Lane 1 : RT-4 cell lysate
Lane 2 : U-251 MG cell lysate
Developed using the ECL technique.
Predicted band size: 136 kDa
Immunofluorescent analysis of U-2 OS cells labeling PPFIA1 with ab204406 at 4 µg/mL. Fixation/Permeabilization: PFA/Triton X-100.
Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling PPFIA1 with ab204406 antibody at 1/2500 dilution.
ab204406 has not yet been referenced specifically in any publications.