Overview

  • Product name
  • Description
    Chicken polyclonal to PPFIA1
  • Host species
    Chicken
  • Specificity
    The antibody has been tested against an e-coli derived fusion protein and has not been tested against endogenous protein.
  • Tested applications
    Suitable for: WB, ICCmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Drosophila melanogaster
    Predicted to work with: Xenopus laevis
  • Immunogen

    Recombinant fragment corresponding to Human PPFIA1 aa 310-360.
    Sequence:

    E AMAQKEDMEE RITTLEKRYL AAQREATSVH DLNDKLENEI ANKDSMHRQT

  • Positive control
    • fusion protein
  • General notes

    Previously labelled as Liprin alpha 1. 

Properties

Applications

Our Abpromise guarantee covers the use of ab26192 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500. Predicted molecular weight: 136 kDa.
ICC 1/200.

Target

  • Function
    May regulate the disassembly of focal adhesions. May localize receptor-like tyrosine phosphatases type 2A at specific sites on the plasma membrane, possibly regulating their interaction with the extracellular environment and their association with substrates.
  • Tissue specificity
    Ubiquitous.
  • Sequence similarities
    Belongs to the liprin family. Liprin-alpha subfamily.
    Contains 3 SAM (sterile alpha motif) domains.
  • Domain
    The N-terminal coiled coil regions mediate homodimerization preferentially and heterodimerization type alpha/alpha. The C-terminal, non-coiled coil regions mediate heterodimerization type alpha/beta and interaction with PTPRD, PTPRF and PTPRS.
  • Cellular localization
    Cytoplasm. Colocalizes with PTPRF at the ends of focal adhesions most proximal to the cell nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ42630 antibody
    • HGNC:9245 antibody
    • LAR interacting protein 1 antibody
    • LAR-interacting protein 1 antibody
    • LIP 1 antibody
    • LIP-1 antibody
    • LIP.1 antibody
    • LIP1 antibody
    • LIPA1_HUMAN antibody
    • Liprin alpha 1 antibody
    • LIPRIN antibody
    • Liprin-alpha-1 antibody
    • MGC26800 antibody
    • PPFIA 1 antibody
    • PPFIA1 antibody
    • Protein tyrosine phosphatase receptor type f polypeptide (PTPRF) interacting protein (liprin) alpha 1 antibody
    • Protein tyrosine phosphatase receptor type f polypeptide interacting protein alpha 1 antibody
    • Protein tyrosine phosphatase receptor type f polypeptide-interacting protein alpha-1 antibody
    • PTPRF interacting protein alpha 1 antibody
    • PTPRF-interacting protein alpha-1 antibody
    see all

References

This product has been referenced in:
  • Lenihan JA  et al. Proteomic analysis reveals novel ligands and substrates for LNX1 E3 ubiquitin ligase. PLoS One 12:e0187352 (2017). Read more (PubMed: 29121065) »
  • Sakamoto S  et al. Liprin-a controls stress fiber formation by binding to mDia and regulating its membrane localization. J Cell Sci 125:108-20 (2012). WB ; Human . Read more (PubMed: 22266902) »
See all 2 Publications for this product

Customer reviews and Q&As

1-6 of 6 Abreviews or Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Drosophila melanogaster Tissue lysate - other (whole embryo)
Gel Running Conditions
Reduced Denaturing (4-20%)
Loading amount
15 µg
Specification
whole embryo
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 23°C

Abcam user community

Verified customer

Submitted Jul 17 2012

Abcam has not validated the combination of species/application used in this Abreview.
Application
IHC - Wholemount
Sample
Drosophila melanogaster Embryo (whole tissue)
Specification
whole tissue

Abcam user community

Verified customer

Submitted Jul 16 2012

Answer

DISCOUNT CODE: ******
Expiration date: *******

I am very pleased to hear you would like to accept our offer and test ab26192 in Drosophila. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview for Drosophila and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.


Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

Read More

Question
Answer

Thank you very much for your interest in ab26192 and please find the details of the 100% Testing Discount that we talked about on the telephone.

To our knowledge, ab26192 has not been tested in Drosophila. Therefore, I can offer a discount off a future purchase if you buy ab26192 now, test it in Drosophila and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free PRIMARY ANTIBODY.

If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed and test ab26192 in Drosophila. I will then send a discount code. This code must be issued before purchasing ab26192 so please wait for my reply before ordering.

2. Purchase ab26192 either by phone, fax, or online (www.abcam.com).

3. Test it in Drosophila.

4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any PRIMARY ANTIBODY ordered and the discount code is valid for 4 months after issue.

We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab26192 turns out to be unsuitable for Drosophila, you will still receive the discount on your next purchase after your Abreview has been submitted.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

Read More

Answer

Thank you for your enquiry. The antibody has not been tested on Drosophila melanogaster and we have not received feedback from customers using this antibody with that species. We do not offer free or trial sized samples for testing purposes. Our policy at Abcam is that if an antibody does not work as specified on the datasheet, we will offer a replacement or reimbursement. If the antibody is being used in an untested species or application, we cannot offer a replacement or refund; however, we invite you to use our Abreview system. The Abreview system allows researchers to submit reviews of our products in exchange for Abpoints. Abpoints can be redeemed for discounts on future antibody purchases or rewards such as Amazon.com gift certificates. Should you decide to go ahead and purchase this product, please let us know how you get on by submitting an Abreview right from the online datasheet. To find out more about our Abreview system, please see the following URL: https://www.abcam.com/abreviews I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

Read More

Answer

Thank you for your enquiry. The reference on the datasheet for ab26192 is a general reference on liprin alpha 1, it does not refer to the antibody I'm afraid. I'm sorry this caused confusion. We are not aware of publications having used this antibody yet, the antibody has only been tested against an E coli-derived fusion protein as test antigen and gave a single band as expected. I'm sorry I do not have further information to help you, please do not hesitate to contact me if I can be of further help,

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up