Product nameAnti-PPFIBP1 antibody
See all PPFIBP1 primary antibodies
DescriptionRabbit polyclonal to PPFIBP1
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Sheep, Rabbit, Horse, Cow, Cat, Dog, Pig, Chimpanzee, Rhesus monkey, Orangutan
- HeLa, Jurkat, 293T, TCMK-1 and NIH/3T3 whole cell lysates.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab195339 was affinity purified using an epitope specific to PPFIBP1 immobilized on solid support.
Our Abpromise guarantee covers the use of ab195339 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 114 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionMay regulate the disassembly of focal adhesions. Did not bind receptor-like tyrosine phosphatases type 2A.
Tissue specificityWidely expressed. Absent in liver.
Sequence similaritiesBelongs to the liprin family. Liprin-beta subfamily.
Contains 3 SAM (sterile alpha motif) domains.
DomainThe N-terminal coiled coil regions mediate homodimerization preferentially and heterodimerization type beta/beta. The C-terminal, non-coiled coil regions mediate heterodimerization type beta/alpha and interaction with S100A4.
- Information by UniProt
- PPFIBP 1 antibody
- hSGT2 antibody
- hSgt2p antibody
All lanes : Anti-PPFIBP1 antibody (ab195339) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate in NETN lysis buffer
Lane 2 : 293T whole cell lysate in NETN lysis buffer
Lane 3 : Jurkat whole cell lysate in NETN lysis buffer
Lane 4 : TCMK-1 whole cell lysate in NETN lysis buffer
Lane 5 : NIH/3T3 whole cell lysate in NETN lysis buffer
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 114 kDa
Exposure time: 10 seconds
Detection of PPFIBP1 in Immunoprecipitates of 293T whole cell lysate prepared using NETN lysis buffer (1 mg for IP, 20% of IP loaded) using ab195339 at 6 µg/mg lysate for IP (Lane 1) and at 0.4 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP.
Detection: Chemiluminescence with an exposure time of 10 seconds.
ab195339 has not yet been referenced specifically in any publications.