May regulate the disassembly of focal adhesions. Did not bind receptor-like tyrosine phosphatases type 2A.
Widely expressed. Absent in liver.
Belongs to the liprin family. Liprin-beta subfamily. Contains 3 SAM (sterile alpha motif) domains.
The N-terminal coiled coil regions mediate homodimerization preferentially and heterodimerization type beta/beta. The C-terminal, non-coiled coil regions mediate heterodimerization type beta/alpha and interaction with S100A4.
Protein tyrosine phosphatase receptor type f polypeptide interacting protein binding protein 1 antibody
Protein tyrosine phosphatase receptor type f polypeptide-interacting protein-binding protein 1 antibody
PTPRF interacting protein binding protein 1 antibody
PTPRF interacting protein, binding protein 1 (liprin beta 1) antibody
PTPRF-interacting protein-binding protein 1 antibody
Western blot - Anti-PPFIBP1 antibody (ab195339)
All lanes : Anti-PPFIBP1 antibody (ab195339) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate in NETN lysis buffer Lane 2 : 293T whole cell lysate in NETN lysis buffer Lane 3 : Jurkat whole cell lysate in NETN lysis buffer Lane 4 : TCMK-1 whole cell lysate in NETN lysis buffer Lane 5 : NIH/3T3 whole cell lysate in NETN lysis buffer
Detection of PPFIBP1 in Immunoprecipitates of 293T whole cell lysate prepared using NETN lysis buffer (1 mg for IP, 20% of IP loaded) using ab195339 at 6 µg/mg lysate for IP (Lane 1) and at 0.4 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP.
Detection: Chemiluminescence with an exposure time of 10 seconds.