Key features and details
- Rabbit polyclonal to PPHLN1/Periphilin-1
- Suitable for: ICC/IF, WB, ELISA, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-PPHLN1/Periphilin-1 antibody
See all PPHLN1/Periphilin-1 primary antibodies
DescriptionRabbit polyclonal to PPHLN1/Periphilin-1
Specificityab69569 detects endogenous levels of total PPHLN protein.
Tested applicationsSuitable for: ICC/IF, WB, ELISA, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
Synthetic peptide corresponding to Human PPHLN1/Periphilin-1 (internal sequence).
- Extracts from COLO or 293 cells. Liver carcinoma tissue.
This product was previously labelled as PPHLN1
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab69569 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|WB||1/500 - 1/1000. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).|
|IHC-P||1/50 - 1/100.|
FunctionInvolved in epithelial differentiation and contributes to epidermal integrity and barrier formation.
modificationsSubstrate of transglutaminase (in vitro).
Cellular localizationNucleus. Cytoplasm. In undifferentiated keratinocytes expressed in speckle-type nuclear granules and at the nuclear membrane, but in the differentiated keratinocytes colocalized with periplakin at the cell periphery and at cell-cell junctions.
- Information by UniProt
- Gastric cancer antigen Ga50 antibody
- Periphilin 1 antibody
- Periphilin-1 antibody
All lanes : Anti-PPHLN1/Periphilin-1 antibody (ab69569) at 1/500 dilution
Lane 1 : Extracts from COLO cells
Lane 2 : Extracts from 293 cells
Lane 3 : Extracts from COLO cells with immunizing peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 53 kDa
Observed band size: 53 kDa
Additional bands at: 100 kDa, 35 kDa, 40 kDa, 55 kDa, 85 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry analysis of paraffin-embedded human liver carcinoma tissue using 1/50-1/100 ab69569. For the image in the right panel, the antibody was preincubated with the immunizing peptide.
ab69569 has been referenced in 2 publications.