Overview

  • Product name

    Anti-PPHLN1/Periphilin-1 antibody
    See all PPHLN1/Periphilin-1 primary antibodies
  • Description

    Rabbit polyclonal to PPHLN1/Periphilin-1
  • Host species

    Rabbit
  • Specificity

    ab69569 detects endogenous levels of total PPHLN protein.
  • Tested applications

    Suitable for: ICC/IF, WB, ELISA, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide corresponding to Human PPHLN1/Periphilin-1 (internal sequence).

  • Positive control

    • Extracts from COLO or 293 cells. Liver carcinoma tissue.
  • General notes

     This product was previously labelled as PPHLN1

     

Properties

Applications

Our Abpromise guarantee covers the use of ab69569 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
WB 1/500 - 1/1000. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).
ELISA 1/20000.
IHC-P 1/50 - 1/100.

Target

  • Function

    Involved in epithelial differentiation and contributes to epidermal integrity and barrier formation.
  • Tissue specificity

    Ubiquitous.
  • Post-translational
    modifications

    Substrate of transglutaminase (in vitro).
  • Cellular localization

    Nucleus. Cytoplasm. In undifferentiated keratinocytes expressed in speckle-type nuclear granules and at the nuclear membrane, but in the differentiated keratinocytes colocalized with periplakin at the cell periphery and at cell-cell junctions.
  • Information by UniProt
  • Database links

  • Alternative names

    • Gastric cancer antigen Ga50 antibody
    • Periphilin 1 antibody
    • Periphilin-1 antibody
    • PPHLN_HUMAN antibody
    • PPHLN1 antibody
    see all

Images

  • All lanes : Anti-PPHLN1/Periphilin-1 antibody (ab69569) at 1/500 dilution

    Lane 1 : Extracts from COLO cells
    Lane 2 : Extracts from 293 cells
    Lane 3 : Extracts from COLO cells with immunizing peptide at 10 µg

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 53 kDa
    Observed band size: 53 kDa
    Additional bands at: 100 kDa, 35 kDa, 40 kDa, 55 kDa, 85 kDa. We are unsure as to the identity of these extra bands.

  • Immunohistochemistry analysis of paraffin-embedded human liver carcinoma tissue using 1/50-1/100 ab69569. For the image in the right panel, the antibody was preincubated with the immunizing peptide.

References

This product has been referenced in:

See all 2 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Permeabilization
Yes - 0.5% Triton X-100
Specification
HeLa
Blocking step
BSA as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Nov 01 2011

Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa)
Loading amount
40 µg
Specification
HeLa
Gel Running Conditions
Reduced Denaturing (8%)
Blocking step
BSA as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Oct 31 2011

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