Recombinant
RabMAb

Recombinant Anti-PPIC antibody [EPR15355] - C-terminal (ab184552)

Overview

  • Product name

    Anti-PPIC antibody [EPR15355] - C-terminal
    See all PPIC primary antibodies
  • Description

    Rabbit monoclonal [EPR15355] to PPIC - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human PPIC aa 150 to the C-terminus. The exact sequence is proprietary.
    Database link: P45877

  • Positive control

    • Human lung carcinoma and mouse cardiac muscle tissues; HeLa, U87-MG and Human fetal kidney lysates; U87-MG cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab184552 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cyt 1/250.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.
  • Sequence similarities

    Belongs to the cyclophilin-type PPIase family.
    Contains 1 PPIase cyclophilin-type domain.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • Cyclophilin C antibody
    • CYPC antibody
    • MGC3673 antibody
    • Parvulin antibody
    • Peptidyl prolyl cis trans isomerase C antibody
    • Peptidyl-prolyl cis-trans isomerase C antibody
    • Peptidylprolyl isomerase C (cyclophilin C) antibody
    • peptidylprolyl isomerase C antibody
    • PPIase C antibody
    • ppiC antibody
    • PPIC_HUMAN antibody
    • Rotamase C antibody
    see all

Images

  • All lanes : Anti-PPIC antibody [EPR15355] - C-terminal (ab184552) at 1/10000 dilution

    Lane 1 : U87-MG cell lysate
    Lane 2 : Human fetal kidney lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 23 kDa

  • Immunohistochemical analysis of paraffin-embedded Human lung carcinoma tissue labeling PPIC with ab184552 at 1/250 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Anti-PPIC antibody [EPR15355] - C-terminal (ab184552) at 1/50000 dilution + HeLa cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 23 kDa

  • Immunohistochemical analysis of paraffin-embedded Mouse cardiac muscle tissue labeling PPIC with ab184552 at 1/250 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Flow cytometric analysis of U87-MG cells (2% paraformaldehyde-fixed) labeling PPIC with ab184552 at 1/250 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.

References

This product has been referenced in:

  • de Wilde AH  et al. Coronaviruses and arteriviruses display striking differences in their cyclophilin A-dependence during replication in cell culture. Virology 517:148-156 (2018). WB . Read more (PubMed: 29249267) »
  • Nguyen D  et al. Proteomics reveals signal peptide features determining the client specificity in human TRAP-dependent ER protein import. Nat Commun 9:3765 (2018). Read more (PubMed: 30217974) »
See all 3 Publications for this product

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