Key features and details
- Rabbit polyclonal to PPIH
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-PPIH antibody
See all PPIH primary antibodies
DescriptionRabbit polyclonal to PPIH
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Cow
Recombinant full length protein corresponding to Human PPIH aa 2-177.
AVANSSPVNPVVFFDVSIGGQEVGRMKIELFADVVPKTAENFRQFCTGEF RKDGVPIGYKGSTFHRVIKDFMIQGGDFVNGDGTGVASIYRGPFADENFK LRHSAPGLLSMANSGPSTNGCQFFITCSKCDWLDGKHVVFGKIIDGLLVM RKIENVPTGPNNKPKLPVVISQCGEM
Database link: O43447
- WB: PC-3 whole cell lysate. IHC-P: Human tonsil tissue.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%.
Our Abpromise guarantee covers the use of ab235595 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/20 - 1/200.|
|WB||1/500 - 1/2000. Predicted molecular weight: 19 kDa.|
FunctionPPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. Participates in pre-mRNA splicing. May play a role in the assembly of the U4/U5/U6 tri-snRNP complex. May act as a chaperone.
Sequence similaritiesBelongs to the cyclophilin-type PPIase family. PPIase H subfamily.
Contains 1 PPIase cyclophilin-type domain.
Cellular localizationNucleus speckle. Cytoplasm. Colocalizes with spliceosomal snRNPs. A small proportion may also be cytoplasmic.
- Information by UniProt
- Cyclophilin H antibody
- CYP-20 antibody
- CypH antibody
Anti-PPIH antibody (ab235595) at 1/500 dilution + PC-3 (human prostate adenocarcinoma cell line) whole cell lysate
Goat polyclonal to rabbit at 1/10000 dilution
Predicted band size: 19 kDa
Paraffin-embedded human tonsil tissue stained for PPIH using ab235595 at 1/100 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab235595 has not yet been referenced specifically in any publications.