Anti-PPIL1 antibody (ab127199)
Key features and details
- Rabbit polyclonal to PPIL1
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PPIL1 antibody
See all PPIL1 primary antibodies -
Description
Rabbit polyclonal to PPIL1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Zebrafish
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Immunogen
Recombinant protein fragment corresponding to a region within amino acids 1 and 116 of Human PPIL1 (UniProt Q9Y3C6).
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Positive control
- U87-MG whole cell lysate, MCF7 cell lysate; MCF7 cell
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab127199 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 18 kDa.
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| ICC/IF |
1/100 - 1/500.
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| Notes |
|---|
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WB
1/500 - 1/3000. Predicted molecular weight: 18 kDa. |
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ICC/IF
1/100 - 1/500. |
Target
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Function
PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. May be involved in pre-mRNA splicing. -
Tissue specificity
Ubiquitous, with the most abundant expression in heart and skeletal muscle. -
Sequence similarities
Belongs to the cyclophilin-type PPIase family. PPIL1 subfamily.
Contains 1 PPIase cyclophilin-type domain. - Information by UniProt
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Database links
- Entrez Gene: 508179 Cow
- Entrez Gene: 51645 Human
- Entrez Gene: 68816 Mouse
- Entrez Gene: 309651 Rat
- Entrez Gene: 558042 Zebrafish
- Omim: 601301 Human
- SwissProt: Q5E992 Cow
- SwissProt: Q5TDC9 Human
see all -
Alternative names
- CGI 124 antibody
- CGI-124 antibody
- CGI124 antibody
see all
Images
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Western blot - Anti-PPIL1 antibody (ab127199)Anti-PPIL1 antibody (ab127199) at 1/1000 dilution + U-87 MG whole cell lysate at 30 µg
Predicted band size: 18 kDa
15% SDS-PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-PPIL1 antibody (ab127199)ab127199, at a 1/500 dilution, staining PPIL1 in paraformaldehyde fixed MCF7 cells by Immunofluorescence.
The lower image was co-stained with Hoechst 33342.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab127199 has not yet been referenced specifically in any publications.