Overview

  • Product name

    Anti-PPM1A antibody [7F12]
    See all PPM1A primary antibodies
  • Description

    Mouse monoclonal [7F12] to PPM1A
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, ELISA, Flow Cytmore details
  • Species reactivity

    Reacts with: Human, Monkey
  • Immunogen

    Purified recombinant fragment corresponding to amino acids 202-382 of Human PPM1A, expressed in E. coli.

  • Positive control

    • Human PPM1A recombinant protein. Cell lysate from PPM1A transfected HEK293 cells. Cell lysates from Jurkat, A431, HeLa, HEK293, Raji, MCF-7 and COS7 cells. HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab135249 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Predicted molecular weight: 42.4 kDa.
ELISA 1/10000.
Flow Cyt 1/200 - 1/400.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    Enzyme with a broad specificity. Negatively regulates TGF-beta signaling through dephosphorylating SMAD2 and SMAD3, resulting in their dissociation from SMAD4, nuclear export of the SMADs and termination of the TGF-beta-mediated signaling.
  • Sequence similarities

    Belongs to the PP2C family.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Protein phosphatase 2C isoform alpha antibody
    • EC 3.1.3.16 antibody
    • FLJ42306 antibody
    • IA antibody
    • MGC9201 antibody
    • Mpp alpha antibody
    • PP2C alpha antibody
    • PP2C-alpha antibody
    • PP2CA antibody
    • PP2Calpha antibody
    • PPM 1A antibody
    • PPM1A antibody
    • PPM1A_HUMAN antibody
    • PPPM1A antibody
    • Protein phosphatase 1A (formerly 2C) magnesium dependent alpha isoform antibody
    • Protein phosphatase 1A antibody
    • Protein phosphatase 1A magnesium dependent alpha antibody
    • Protein phosphatase 2C alpha antibody
    • Protein phosphatase 2C alpha isoform antibody
    • Protein phosphatase 2C isoform alpha antibody
    • Protein phosphatase IA antibody
    • Protein phosphatase, Mg2+/Mn2+ dependent, 1A antibody
    see all

Images

  • Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: PPM1A knockout HAP1 cell lysate (20 µg)
    Lane 3: HeLa cell lysate (20 µg)
    Lane 4: Jurkat cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab135249 observed at 42 kDa. Red - loading control, ab18251, observed at 37 kDa.

    ab135249 was shown to specifically react with PPM1A when PPM1A knockout samples were used. Wild-type and PPM1A knockout samples were subjected to SDS-PAGE. ab135249 at a dilution of 1/500 and ab18251 (loading control to alpha Tubulin) at a dilution of 1/10,000 were incubated overnight at 4°C. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772 and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777 secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

  • ELISA analysis using ab135249 with different concentrations of antigen.
  • Anti-PPM1A antibody [7F12] (ab135249) at 1/500 dilution + Human PPM1A recombinant protein.

    Predicted band size: 42.4 kDa

  • All lanes : Anti-PPM1A antibody [7F12] (ab135249) at 1/500 dilution

    Lane 1 : HEK293 cell lysate
    Lane 2 : PPM1A (amino acids 202-382)-hIgGFc transfected HEK293 cell lysate.

    Predicted band size: 42.4 kDa

  • All lanes : Anti-PPM1A antibody [7F12] (ab135249) at 1/500 dilution

    Lanes 1-2 : Jurkat cell lysate
    Lane 3 : A431 cell lysate
    Lane 4 : HeLa cell lysate
    Lane 5 : HEK293 cell lysate
    Lane 6 : Raji cell lysate
    Lane 7 : MCF-7 cell lysate
    Lane 8 : COS7 cell lysate

    Predicted band size: 42.4 kDa

  • Flow cytometric analysis of HeLa cells using ab135249 at 1/200 dilution (green) or a negative control (red).

References

ab135249 has not yet been referenced specifically in any publications.

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