Key features and details
- Rabbit polyclonal to PPM1B
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-PPM1B antibody
See all PPM1B primary antibodies
DescriptionRabbit polyclonal to PPM1B
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan
Synthetic peptide corresponding to a region between residue 450 and the C-terminus (residue 479) of Human PPM1B (NP_002697.)
- Whole cell lysate from HeLa, 293T and NIH3T3 cells.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab70804 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).|
|IP||Use at 2-5 µg/mg of lysate.|
FunctionEnzyme with a broad specificity. Dephosphorylates CDK2 and CDK6 in vitro.
Tissue specificityHighly expressed in heart and skeletal muscle.
Sequence similaritiesBelongs to the PP2C family.
- Information by UniProt
- MGC21657 antibody
- OTTHUMP00000158953 antibody
- OTTHUMP00000158954 antibody
All lanes : Anti-PPM1B antibody (ab70804) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 53 kDa
Observed band size: 53 kDa
Exposure time: 3 minutes
Detection of PPM1B by Western Blot of Immunoprecipitate.
1mg whole cell lysate from HeLa cells was immunoprecipitated using ab70804 at 3ug/mg of lysate. for the subsequent western blot, 20% of the immunoprecipitate was probed with ab70804 at 1ug/ml.
Detection: chemiluminescence with exposure time of 10 seconds.
ab70804 has been referenced in 2 publications.
- Zhu H et al. TNF-a induces apoptosis of human nucleus pulposus cells via activating the TRIM14/NF-?B signalling pathway. Artif Cells Nanomed Biotechnol 47:3004-3012 (2019). PubMed: 31322007
- Putz EM et al. Novel non-canonical role of STAT1 in Natural Killer cell cytotoxicity. Oncoimmunology 5:e1186314 (2016). PubMed: 27757297