Overview

  • Product name
  • Description
    Rabbit polyclonal to PPM1D
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Xenopus laevis, Zebrafish
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 200 - 300 of Human PPM1D.

    Read Abcam's proprietary immunogen policy (Peptide available as ab32657.)

  • Positive control
    • This antibody gave a positive signal in the following whole cell lysates: Jurkat and HEK293 in WB.

Properties

Applications

Our Abpromise guarantee covers the use of ab31270 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 67 kDa (predicted molecular weight: 67 kDa).

Target

  • Function
    Required for the relief of p53-dependent checkpoint mediated cell cycle arrest. Binds to and dephosphorylates 'Ser-15' of TP53 and 'Ser-345' of CHEK1 which contributes to the functional inactivation of these proteins.
  • Sequence similarities
    Belongs to the PP2C family.
    Contains 1 PP2C-like domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • EC 3.1.3.16 antibody
    • p53 induced protein phosphatase 1 antibody
    • p53-induced protein phosphatase 1 antibody
    • PP2C delta antibody
    • PP2C-delta antibody
    • PP2CD antibody
    • Ppm1d antibody
    • PPM1D_HUMAN antibody
    • Protein phosphatase 1D antibody
    • protein phosphatase 1D magnesium-dependent delta isoform antibody
    • Protein phosphatase 2C delta isoform antibody
    • Protein phosphatase 2C isoform delta antibody
    • Protein phosphatase magnesium dependent 1 delta antibody
    • Protein phosphatase magnesium-dependent 1 delta antibody
    • Protein phosphatase Mg2+/Mn2+ dependent 1D antibody
    • Protein phosphatase Wip1 antibody
    • Wild type p53 induced phosphatase 1 antibody
    • WIP 1 antibody
    • WIP1 antibody
    see all

Images

  • All lanes : Anti-PPM1D antibody (ab31270) at 1 µg/ml

    Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 67 kDa
    Observed band size: 67 kDa


    Exposure time: 20 minutes

References

This product has been referenced in:
  • Wang ZP  et al. Wild-type p53-induced phosphatase 1 is a prognostic marker and therapeutic target in bladder transitional cell carcinoma. Oncol Lett 13:875-880 (2017). Read more (PubMed: 28356972) »
  • Li GB  et al. Protein phosphatase magnesium-dependent 1d (PPM1D) mRNA expression is a prognosis marker for hepatocellular carcinoma. PLoS One 8:e60775 (2013). IHC-P ; Human . Read more (PubMed: 23556002) »
See all 3 Publications for this product

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Mouse Cell (Primary cells, testicular germ cells))
Permeabilization
Yes - Triton X-100
Specification
Mouse Cell (Primary cells, testicular germ cells)
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 0.5% · Temperature: RT°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Sep 22 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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