Recombinant Anti-PPM1E antibody [EPR8721] (ab137122)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8721] to PPM1E
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-PPM1E antibody [EPR8721]
See all PPM1E primary antibodies -
Description
Rabbit monoclonal [EPR8721] to PPM1E -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt or ICC/IF -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human PPM1E aa 200-300. The exact sequence is proprietary.
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Positive control
- SH-SY5Y, Human hypothalamus, and Human hippocampus lysates, Human brain tissue
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR8721 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab137122 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Detects a band of approximately 84 kDa (predicted molecular weight: 85 kDa).
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 84 kDa (predicted molecular weight: 85 kDa). |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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Function
Protein phosphatase that inactivates multifunctional CaM kinases such as CAMK4 and CAMK2 (By similarity). Dephosphorylates and inactivates PAK. May play a role in the inhibition of actin fiber stress breakdown and in morphological changes driven by TNK2/CDC42. -
Sequence similarities
Belongs to the PP2C family.
Contains 1 PP2C-like domain. -
Cellular localization
Nucleus. Cytoplasm. A truncated form, major form, with the C-terminal part missing, is mostly found in the cytoplasm and a little in the nucleus. The full length, minor form, is found in the nucleus. - Information by UniProt
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Database links
- Entrez Gene: 22843 Human
- SwissProt: Q8WY54 Human
- Unigene: 245044 Human
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Alternative names
- Ca(2+)/calmodulin-dependent protein kinase phosphatase N antibody
- CaMKN antibody
- CaMKP-N antibody
see all
Images
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All lanes : Anti-PPM1E antibody [EPR8721] (ab137122) at 1/1000 dilution
Lane 1 : SH-SY5Y lysate
Lane 2 : Human hypothalamus lysate
Lane 3 : Human hippocampus lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 85 kDa
Observed band size: 84 kDa why is the actual band size different from the predicted? -
Immunohistochemical analysis of paraffin embedded Human brain tissue labelling PPM1E with ab137122 antibody at a dilution of 1/50.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab137122 has been referenced in 1 publication.
- Jie W et al. Inhibition of cell proliferation by Tas of foamy viruses through cell cycle arrest or apoptosis underlines the different mechanisms of virus-host interactions. Virulence 13:342-354 (2022). PubMed: 35132916