Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8721] to PPM1E
- Suitable for: WB, IHC-P, IP
- Reacts with: Human
Product nameAnti-PPM1E antibody [EPR8721]
See all PPM1E primary antibodies
DescriptionRabbit monoclonal [EPR8721] to PPM1E
Tested applicationsSuitable for: WB, IHC-P, IPmore details
Unsuitable for: Flow Cyt or ICC/IF
Species reactivityReacts with: Human
Synthetic peptide within Human PPM1E aa 200-300. The exact sequence is proprietary.
- SH-SY5Y, Human hypothalamus, and Human hippocampus lysates, Human brain tissue
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab137122 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 84 kDa (predicted molecular weight: 85 kDa).|
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|IP||1/10 - 1/100.|
FunctionProtein phosphatase that inactivates multifunctional CaM kinases such as CAMK4 and CAMK2 (By similarity). Dephosphorylates and inactivates PAK. May play a role in the inhibition of actin fiber stress breakdown and in morphological changes driven by TNK2/CDC42.
Sequence similaritiesBelongs to the PP2C family.
Contains 1 PP2C-like domain.
Cellular localizationNucleus. Cytoplasm. A truncated form, major form, with the C-terminal part missing, is mostly found in the cytoplasm and a little in the nucleus. The full length, minor form, is found in the nucleus.
- Information by UniProt
- Ca(2+)/calmodulin-dependent protein kinase phosphatase N antibody
- CaMKN antibody
- CaMKP-N antibody
All lanes : Anti-PPM1E antibody [EPR8721] (ab137122) at 1/1000 dilution
Lane 1 : SH-SY5Y lysate
Lane 2 : Human hypothalamus lysate
Lane 3 : Human hippocampus lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 85 kDa
Observed band size: 84 kDa why is the actual band size different from the predicted?
Immunohistochemical analysis of paraffin embedded Human brain tissue labelling PPM1E with ab137122 antibody at a dilution of 1/50.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ab137122 has not yet been referenced specifically in any publications.