Product nameAnti-PPM1E antibody [EPR8721]
See all PPM1E primary antibodies
DescriptionRabbit monoclonal [EPR8721] to PPM1E
Tested applicationsSuitable for: WB, IHC-P, IPmore details
Unsuitable for: Flow Cyt or ICC/IF
Species reactivityReacts with: Human
Synthetic peptide within Human PPM1E aa 200-300. The exact sequence is proprietary.
- SH-SY5Y, Human hypothalamus, and Human hippocampus lysates, Human brain tissue
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab137122 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 84 kDa (predicted molecular weight: 85 kDa).|
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|IP||1/10 - 1/100.|
FunctionProtein phosphatase that inactivates multifunctional CaM kinases such as CAMK4 and CAMK2 (By similarity). Dephosphorylates and inactivates PAK. May play a role in the inhibition of actin fiber stress breakdown and in morphological changes driven by TNK2/CDC42.
Sequence similaritiesBelongs to the PP2C family.
Contains 1 PP2C-like domain.
Cellular localizationNucleus. Cytoplasm. A truncated form, major form, with the C-terminal part missing, is mostly found in the cytoplasm and a little in the nucleus. The full length, minor form, is found in the nucleus.
- Information by UniProt
- Ca(2+)/calmodulin-dependent protein kinase phosphatase N antibody
- CaMKN antibody
- CaMKP-N antibody
All lanes : Anti-PPM1E antibody [EPR8721] (ab137122) at 1/1000 dilution
Lane 1 : SH-SY5Y lysate
Lane 2 : Human hypothalamus lysate
Lane 3 : Human hippocampus lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 85 kDa
Observed band size: 84 kDa why is the actual band size different from the predicted?
Immunohistochemical analysis of paraffin embedded Human brain tissue labelling PPM1E with ab137122 antibody at a dilution of 1/50.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ab137122 has not yet been referenced specifically in any publications.