Overview

  • Product name

    Anti-PPM1E antibody [EPR8721]
    See all PPM1E primary antibodies
  • Description

    Rabbit monoclonal [EPR8721] to PPM1E
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, IPmore details
    Unsuitable for: Flow Cyt or ICC/IF
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human PPM1E aa 200-300. The exact sequence is proprietary.

  • Positive control

    • SH-SY5Y, Human hypothalamus, and Human hippocampus lysates, Human brain tissue
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab137122 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 84 kDa (predicted molecular weight: 85 kDa).
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt or ICC/IF.
  • Target

    • Function

      Protein phosphatase that inactivates multifunctional CaM kinases such as CAMK4 and CAMK2 (By similarity). Dephosphorylates and inactivates PAK. May play a role in the inhibition of actin fiber stress breakdown and in morphological changes driven by TNK2/CDC42.
    • Sequence similarities

      Belongs to the PP2C family.
      Contains 1 PP2C-like domain.
    • Cellular localization

      Nucleus. Cytoplasm. A truncated form, major form, with the C-terminal part missing, is mostly found in the cytoplasm and a little in the nucleus. The full length, minor form, is found in the nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • Ca(2+)/calmodulin-dependent protein kinase phosphatase N antibody
      • CaMKN antibody
      • CaMKP-N antibody
      • CaMKP-nucleus antibody
      • KIAA1072 antibody
      • Nuclear calmodulin dependent protein kinase phosphatase antibody
      • Partner of PIX 1 antibody
      • Partner of PIX-alpha antibody
      • Partner of PIXA antibody
      • POPX1 antibody
      • PP2CH antibody
      • Ppm1e antibody
      • PPM1E_HUMAN antibody
      • Protein phosphatase 1E antibody
      • Protein phosphatase Mg2+/Mn2+ dependent 1E antibody
      see all

    Images

    • All lanes : Anti-PPM1E antibody [EPR8721] (ab137122) at 1/1000 dilution

      Lane 1 : SH-SY5Y lysate
      Lane 2 : Human hypothalamus lysate
      Lane 3 : Human hippocampus lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 85 kDa
      Observed band size: 84 kDa
      why is the actual band size different from the predicted?

    • Immunohistochemical analysis of paraffin embedded Human brain tissue labelling PPM1E with ab137122 antibody at a dilution of 1/50.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    References

    ab137122 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab137122.
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