Product nameAnti-PPM1E antibody [EPR8722]
See all PPM1E primary antibodies
DescriptionRabbit monoclonal [EPR8722] to PPM1E
Tested applicationsSuitable for: WB, ICC/IF, IP, Flow Cytmore details
Unsuitable for: IHC-P
Species reactivityReacts with: Human
Synthetic peptide within Human PPM1E aa 500-600. The exact sequence is proprietary.
- SH-SY5Y, Human fetal brain and Human cerebellum lysates; SH-SY5Y cells
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at -20ºC.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab137023 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 85 kDa.|
|ICC/IF||1/100 - 1/250.|
|IP||1/10 - 1/100.|
|Flow Cyt||1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionProtein phosphatase that inactivates multifunctional CaM kinases such as CAMK4 and CAMK2 (By similarity). Dephosphorylates and inactivates PAK. May play a role in the inhibition of actin fiber stress breakdown and in morphological changes driven by TNK2/CDC42.
Sequence similaritiesBelongs to the PP2C family.
Contains 1 PP2C-like domain.
Cellular localizationNucleus. Cytoplasm. A truncated form, major form, with the C-terminal part missing, is mostly found in the cytoplasm and a little in the nucleus. The full length, minor form, is found in the nucleus.
- Information by UniProt
- Ca(2+)/calmodulin-dependent protein kinase phosphatase N antibody
- CaMKN antibody
- CaMKP-N antibody
All lanes : Anti-PPM1E antibody [EPR8722] (ab137023) at 1/1000 dilution
Lane 1 : SH-SY5Y lysate
Lane 2 : Human fetal brain lysate
Lane 3 : Human cerebellum lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 85 kDa
Flow Cytometric analysis of permeabilized SH-SY5Y cells labelling PPM1E with ab137023 at 1/100 dilution (red). Rabbit IgG negative control is green.
ab137023 has not yet been referenced specifically in any publications.