Anti-PPM1G antibody (ab70794)
Key features and details
- Rabbit polyclonal to PPM1G
- Suitable for: IHC-P, WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-PPM1G antibody
See all PPM1G primary antibodies -
Description
Rabbit polyclonal to PPM1G -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, IPmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Xenopus laevis, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis, Platypus
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Immunogen
Synthetic peptide corresponding to a region between residue 400 and 450 of human PPM1G. (NP_817092.1)
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Positive control
- HeLa and 293T whole cell lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab70794 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| WB |
1/2000 - 1/10000. Detects a band of approximately 70 kDa (predicted molecular weight: 61 kDa).
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| IP |
Use at 2-5 µg/mg of lysate.
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| Notes |
|---|
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IHC-P
1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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WB
1/2000 - 1/10000. Detects a band of approximately 70 kDa (predicted molecular weight: 61 kDa). |
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IP
Use at 2-5 µg/mg of lysate. |
Target
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Tissue specificity
Widely expressed. Most abundant in testis, skeletal muscle, and heart. -
Sequence similarities
Belongs to the PP2C family. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 286880 Cow
- Entrez Gene: 5496 Human
- Entrez Gene: 14208 Mouse
- Omim: 605119 Human
- SwissProt: P79126 Cow
- SwissProt: O15355 Human
- SwissProt: Q61074 Mouse
- Unigene: 643951 Human
see all -
Alternative names
- MGC1675 antibody
- MGC2870 antibody
- OTTHUMP00000123426 antibody
see all
Images
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Western blot - Anti-PPM1G antibody (ab70794)All lanes : Anti-PPM1G antibody (ab70794) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 61 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?
Additional bands at: 130 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 10 seconds -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPM1G antibody (ab70794)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skin basal cell carcinoma (left) and mouse squamous cell carcinoma (right) tissues labelling PPM1G with ab70794 at 1/1000 (0.2µg/ml) and 1/200 (1µg/ml). Detection: DAB. -
Immunoprecipitation - Anti-PPM1G antibody (ab70794)HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) with ab70794 at 1 µg/ml for WB, subsequent to being used for IP at 3 µg/mg lysate.
Detection: Chemiluminescence with an exposure time of 1 second.
Protocols
Datasheets and documents
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Datasheet download
References (3)
ab70794 has been referenced in 3 publications.
- Pyo J et al. The Protein Phosphatase PPM1G Destabilizes HIF-1a Expression. Int J Mol Sci 19:N/A (2018). PubMed: 30081604
- McNamara RP et al. Transcription factors mediate the enzymatic disassembly of promoter-bound 7SK snRNP to locally recruit P-TEFb for transcription elongation. Cell Rep 5:1256-68 (2013). ChIP ; Human . PubMed: 24316072
- Sun L et al. Characterization of the interaction of Aha1 with components of the Hsp90 chaperone machine and client proteins. Biochim Biophys Acta 1823:1092-101 (2012). PubMed: 22504172
