Anti-PPM1G antibody (ab70794)
Key features and details
- Rabbit polyclonal to PPM1G
- Suitable for: IHC-P, WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-PPM1G antibody
See all PPM1G primary antibodies -
Description
Rabbit polyclonal to PPM1G -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P MouseHumanIP HumanWB Human
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Immunogen
Synthetic peptide corresponding to a region between residue 400 and 450 of human PPM1G. (NP_817092.1)
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Positive control
- HeLa and 293T whole cell lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab70794 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Guaranteed
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted
Predicted to work for this combination of applications and species but not guaranteed.
Incompatible
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| IHC-P |
Mouse
Human
|
| IP |
Human
|
| WB |
Human
|
| All applications |
Rabbit
Horse
Chicken
Guinea pig
Cow
Dog
Turkey
Pig
Xenopus laevis
Chimpanzee
Rhesus monkey
Gorilla
Orangutan
Xenopus tropicalis
Platypus
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| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
|
| WB |
1/2000 - 1/10000. Detects a band of approximately 70 kDa (predicted molecular weight: 61 kDa).
|
|
| IP |
Use at 2-5 µg/mg of lysate.
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| Notes |
|---|
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IHC-P
1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
WB
1/2000 - 1/10000. Detects a band of approximately 70 kDa (predicted molecular weight: 61 kDa). |
|
IP
Use at 2-5 µg/mg of lysate. |
Target
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Tissue specificity
Widely expressed. Most abundant in testis, skeletal muscle, and heart. -
Sequence similarities
Belongs to the PP2C family. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 286880 Cow
- Entrez Gene: 5496 Human
- Entrez Gene: 14208 Mouse
- Omim: 605119 Human
- SwissProt: P79126 Cow
- SwissProt: O15355 Human
- SwissProt: Q61074 Mouse
- Unigene: 643951 Human
see all -
Alternative names
- MGC1675 antibody
- MGC2870 antibody
- OTTHUMP00000123426 antibody
see all
Images
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Western blot - Anti-PPM1G antibody (ab70794)All lanes : Anti-PPM1G antibody (ab70794) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 61 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?
Additional bands at: 130 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 10 seconds -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPM1G antibody (ab70794)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skin basal cell carcinoma (left) and mouse squamous cell carcinoma (right) tissues labelling PPM1G with ab70794 at 1/1000 (0.2µg/ml) and 1/200 (1µg/ml). Detection: DAB. -
Immunoprecipitation - Anti-PPM1G antibody (ab70794)HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) with ab70794 at 1 µg/ml for WB, subsequent to being used for IP at 3 µg/mg lysate.
Detection: Chemiluminescence with an exposure time of 1 second.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab70794 has been referenced in 3 publications.
- Pyo J et al. The Protein Phosphatase PPM1G Destabilizes HIF-1a Expression. Int J Mol Sci 19:N/A (2018). PubMed: 30081604
- McNamara RP et al. Transcription factors mediate the enzymatic disassembly of promoter-bound 7SK snRNP to locally recruit P-TEFb for transcription elongation. Cell Rep 5:1256-68 (2013). ChIP ; Human . PubMed: 24316072
- Sun L et al. Characterization of the interaction of Aha1 with components of the Hsp90 chaperone machine and client proteins. Biochim Biophys Acta 1823:1092-101 (2012). PubMed: 22504172