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    ppm1g-antibody-ab70794.pdf

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Cell Biology Cell Cycle Kinases/Phosphatases Phosphatases
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Anti-PPM1G antibody (ab70794)

  • Datasheet
  • SDS
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Western blot - Anti-PPM1G antibody (ab70794)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPM1G antibody (ab70794)
  • Immunoprecipitation - Anti-PPM1G antibody (ab70794)

Key features and details

  • Rabbit polyclonal to PPM1G
  • Suitable for: IHC-P, WB, IP
  • Reacts with: Mouse, Human
  • Isotype: IgG

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Recombinant human PPM1G protein (ab128553)
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Human PPM1G knockout HEK-293T cell line (ab266162)

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Overview

  • Product name

    Anti-PPM1G antibody
    See all PPM1G primary antibodies
  • Description

    Rabbit polyclonal to PPM1G
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    IHC-P
    Mouse
    Human
    IP
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide corresponding to a region between residue 400 and 450 of human PPM1G. (NP_817092.1)

  • Positive control

    • HeLa and 293T whole cell lysates.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 6.8
    Preservative: 0.09% Sodium azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Kinases/Phosphatases
    • Phosphatases
    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Phosphatases
    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Kinases/Phosphatases
    • Phospatases

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HeLa whole cell lysate (ab29545)
  • Recombinant Protein

    • Recombinant human PPM1G protein (ab128553)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab70794 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IHC-P
Mouse
Human
IP
Human
WB
Human
All applications
Rabbit
Horse
Chicken
Guinea pig
Cow
Dog
Turkey
Pig
Xenopus laevis
Chimpanzee
Rhesus monkey
Gorilla
Orangutan
Xenopus tropicalis
Platypus
Application Abreviews Notes
IHC-P
1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
1/2000 - 1/10000. Detects a band of approximately 70 kDa (predicted molecular weight: 61 kDa).
IP
Use at 2-5 µg/mg of lysate.
Notes
IHC-P
1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
1/2000 - 1/10000. Detects a band of approximately 70 kDa (predicted molecular weight: 61 kDa).
IP
Use at 2-5 µg/mg of lysate.

Target

  • Tissue specificity

    Widely expressed. Most abundant in testis, skeletal muscle, and heart.
  • Sequence similarities

    Belongs to the PP2C family.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Cytoplasm.
  • Target information above from: UniProt accession O15355 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 286880 Cow
    • Entrez Gene: 5496 Human
    • Entrez Gene: 14208 Mouse
    • Omim: 605119 Human
    • SwissProt: P79126 Cow
    • SwissProt: O15355 Human
    • SwissProt: Q61074 Mouse
    • Unigene: 643951 Human
    • Unigene: 14501 Mouse
    see all
  • Alternative names

    • MGC1675 antibody
    • MGC2870 antibody
    • OTTHUMP00000123426 antibody
    • PP2C antibody
    • PP2C gamma antibody
    • PP2C-gamma antibody
    • PP2CG antibody
    • PP2Cgamma antibody
    • PPM1G antibody
    • PPM1G_HUMAN antibody
    • PPP2CG antibody
    • Protein phosphatase 1C antibody
    • Protein phosphatase 1G antibody
    • Protein phosphatase 2 catalytic subunit gamma isoform antibody
    • Protein phosphatase 2C gamma isoform antibody
    • Protein phosphatase 2C isoform gamma antibody
    • Protein phosphatase magnesium dependent 1 gamma antibody
    • Protein phosphatase magnesium-dependent 1 gamma antibody
    • Protein phosphatase Mg2+/Mn2+ dependent 1G antibody
    see all

Images

  • Western blot - Anti-PPM1G antibody (ab70794)
    Western blot - Anti-PPM1G antibody (ab70794)
    All lanes : Anti-PPM1G antibody (ab70794) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 61 kDa
    Observed band size: 70 kDa why is the actual band size different from the predicted?
    Additional bands at: 130 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 10 seconds
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPM1G antibody (ab70794)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPM1G antibody (ab70794)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skin basal cell carcinoma (left) and mouse squamous cell carcinoma (right) tissues labelling PPM1G with ab70794 at 1/1000 (0.2µg/ml) and 1/200 (1µg/ml). Detection: DAB.
  • Immunoprecipitation - Anti-PPM1G antibody (ab70794)
    Immunoprecipitation - Anti-PPM1G antibody (ab70794)
    HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) with ab70794 at 1 µg/ml for WB, subsequent to being used for IP at 3 µg/mg lysate.
    Detection: Chemiluminescence with an exposure time of 1 second.

Protocols

  • Western blot protocols
  • Immunoprecipitation protocols
  • Immunohistochemistry protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (3)

Publishing research using ab70794? Please let us know so that we can cite the reference in this datasheet.

ab70794 has been referenced in 3 publications.

  • Pyo J  et al. The Protein Phosphatase PPM1G Destabilizes HIF-1a Expression. Int J Mol Sci 19:N/A (2018). PubMed: 30081604
  • McNamara RP  et al. Transcription factors mediate the enzymatic disassembly of promoter-bound 7SK snRNP to locally recruit P-TEFb for transcription elongation. Cell Rep 5:1256-68 (2013). ChIP ; Human . PubMed: 24316072
  • Sun L  et al. Characterization of the interaction of Aha1 with components of the Hsp90 chaperone machine and client proteins. Biochim Biophys Acta 1823:1092-101 (2012). PubMed: 22504172

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