Overview

  • Product name

    Anti-PPM1G antibody [EPR13362-31]
    See all PPM1G primary antibodies
  • Description

    Rabbit monoclonal [EPR13362-31] to PPM1G
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human PPM1G aa 300-450. The exact sequence is proprietary.
    Database link: O15355

  • Positive control

    • 293T, Jurkat, HeLa and MCF7 cell lysates; Human kidney clear cell carcinoma and Mouse cardiac muscle tissues; HeLa, 293T and 293 cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab186423 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 75 kDa (predicted molecular weight: 59 kDa).
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF 1/100.
Flow Cyt 1/200.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Tissue specificity

    Widely expressed. Most abundant in testis, skeletal muscle, and heart.
  • Sequence similarities

    Belongs to the PP2C family.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • MGC1675 antibody
    • MGC2870 antibody
    • OTTHUMP00000123426 antibody
    • PP2C antibody
    • PP2C gamma antibody
    • PP2C-gamma antibody
    • PP2CG antibody
    • PP2Cgamma antibody
    • PPM1G antibody
    • PPM1G_HUMAN antibody
    • PPP2CG antibody
    • Protein phosphatase 1C antibody
    • Protein phosphatase 1G antibody
    • Protein phosphatase 2 catalytic subunit gamma isoform antibody
    • Protein phosphatase 2C gamma isoform antibody
    • Protein phosphatase 2C isoform gamma antibody
    • Protein phosphatase magnesium dependent 1 gamma antibody
    • Protein phosphatase magnesium-dependent 1 gamma antibody
    • Protein phosphatase Mg2+/Mn2+ dependent 1G antibody
    see all

Images

  • All lanes : Anti-PPM1G antibody [EPR13362-31] (ab186423) at 1/10000 dilution

    Lane 1 : 293T cell lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : MCF7 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 59 kDa

  • Immunohistochemical analysis of paraffin-embedded Human kidney clear cell carcinoma tissue labeling PPM1G with ab186423 at 1/500 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded Mouse cardiac muscle tissue labeling PPM1G with ab186423 at 1/500 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling PPM1G with ab186423 at 1/500 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody at 1/200 dilution (Green). Counter stained with Dapi (Blue).

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed 293T cells labeling PPM1G with ab186423 at 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody at 1/200 dilution. Counter stained with Dapi.

  • Flow cytometric analysis of 2% paraformaldehyde-fixed 293 cells labeling PPM1G with ab186423 at 1/200 dilution (red) compared to a Rabbit monoclonal IgG isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.

References

ab186423 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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