Overview

  • Product name

  • Description

    Rabbit polyclonal to PPM1K
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Horse, Dog, Chimpanzee, Macaque monkey, Gorilla, Chinese hamster, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human PPM1K aa 50-150 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab166587)

  • Positive control

    • This antibody gave a positive signal in the following tissue lysates: Human Heart; Mouse Heart; Human Skeletal Muscle; Mouse Skeletal Muscle. IHC-P: Human heart muscle FFPE tissue sections.

Properties

Applications

Our Abpromise guarantee covers the use of ab135286 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 40 kDa).

Target

  • Function

    Regulates the mitochondrial permeability transition pore and is essential for cellular survival and development.
  • Sequence similarities

    Belongs to the PP2C family.
    Contains 1 PP2C-like domain.
  • Cellular localization

    Mitochondrion matrix.
  • Information by UniProt
  • Database links

  • Alternative names

    • BDP antibody
    • mitochondrial antibody
    • MSUDMV antibody
    • PP2C domain containing protein phosphatase 1K antibody
    • PP2C domain-containing protein phosphatase 1K antibody
    • PP2C kappa antibody
    • PP2C like mitochondrial protein antibody
    • PP2C type mitochondrial phosphoprotein phosphatase antibody
    • PP2C-kappa antibody
    • PP2C-like mitochondrial protein antibody
    • PP2C-type mitochondrial phosphoprotein phosphatase antibody
    • PP2Ckappa antibody
    • PP2CM antibody
    • PPM1K antibody
    • PPM1K_HUMAN antibody
    • Protein phosphatase 1K antibody
    • Protein phosphatase 1K, mitochondrial antibody
    • Protein phosphatase 2C isoform kappa antibody
    • Protein phosphatase Mg2+/Mn2+ dependent 1K antibody
    • PTMP antibody
    • UG0882E07 antibody
    see all

Images

  • IHC image of PPMK1 staining in human heart formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab135286, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • All lanes : Anti-PPM1K antibody (ab135286) at 1 µg/ml

    Lane 1 : Human heart tissue lysate - total protein (ab29431)
    Lane 2 : Heart (Mouse) Tissue Lysate
    Lane 3 : Human skeletal muscle tissue lysate - total protein (ab29330)
    Lane 4 : Skeletal Muscle (Mouse) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 40 kDa
    Observed band size: 37 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 70 kDa, 99 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 90 seconds


    The band observed at 37 kDa could potentially be a cleaved form of PPM1K due to the presence of a 29 amino acid transit peptide. This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab135286 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

References

ab135286 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Rat Tissue lysate - nuclear (brain)
Gel Running Conditions
Reduced Denaturing (NuPAGE® Novex® 4-12% Bis-Tris Protein Gels, 1.0 mm, 12 well)
Loading amount
30 µg
Specification
brain
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C

Abcam user community

Verified customer

Submitted Sep 29 2015

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