Key features and details
- Rabbit polyclonal to PPM1K
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-PPM1K antibody
See all PPM1K primary antibodies
DescriptionRabbit polyclonal to PPM1K
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Cow
- WB: PPM1K overexpression HEK-293T cell lysate. IHC-P: Human urinary bladder tissue. ICC/IF: U-2 OS cells.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab224424 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/100 - 1/250. Predicted molecular weight: 41 kDa.|
|IHC-P||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100.
FunctionRegulates the mitochondrial permeability transition pore and is essential for cellular survival and development.
Sequence similaritiesBelongs to the PP2C family.
Contains 1 PP2C-like domain.
Cellular localizationMitochondrion matrix.
- Information by UniProt
- BDP antibody
- mitochondrial antibody
- MSUDMV antibody
PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for PPM1K (green) using ab224424 at 4 µg/ml in ICC/IF.
Paraffin-embedded human urinary bladder tissue stained for PPM1K using ab224424 at 1/200 dilution in immunohistochemical analysis.
All lanes : Anti-PPM1K antibody (ab224424) at 1/100 dilution
Lane 1 : Vector only transfected HEK-293T (human epithelial cell line from embryonic kidney transformed wth a large T antigen) cell lysate
Lane 2 : PPM1K overexpression HEK-293T cell lysate (Co-expressed with a C-terminal myc-DDK tag, ~3.1 kDa)
Predicted band size: 41 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab224424 has not yet been referenced specifically in any publications.