Overview

  • Product name
  • Description
    Rabbit polyclonal to PPME1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Cow
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 18-297 of Human PPME1.

  • Positive control
    • 293T, A431, HeLa, HepG2, NIH 3T3, JC and BCL1 whole cell lysates; Human Hepatoma tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 79% PBS, 20% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab154569 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 42 kDa.
IHC-P 1/100 - 1/1000.

Target

Images

  • All lanes : Anti-PPME1 antibody (ab154569) at 1/1000 dilution

    Lane 1 : NIH 3T3 whole cell lysate
    Lane 2 : JC whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 42 kDa



    10% SDS PAGE
  • All lanes : Anti-PPME1 antibody (ab154569) at 1/1000 dilution

    Lane 1 : 293T whole cell lysate
    Lane 2 : A431 whole cell lysate
    Lane 3 : HeLa whole cell lysate
    Lane 4 : HepG2 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 42 kDa



    10% SDS PAGE
  • Immunohistochemical analysis of paraffin-embedded Human Hepatoma tissue labeling PPME1 with ab154569 at 1/500 dilution.

References

This product has been referenced in:
  • Little SC  et al. Protein phosphatase 2A regulatory subunit B56a limits phosphatase activity in the heart. Sci Signal 8:ra72 (2015). Read more (PubMed: 26198358) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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