Key features and details
- Rabbit polyclonal to PPOX
- Suitable for: WB, IP, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-PPOX antibody
See all PPOX primary antibodies
DescriptionRabbit polyclonal to PPOX
Tested applicationsSuitable for: WB, IP, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Cow, Cynomolgus monkey
Recombinant full length protein corresponding to Human PPOX aa 1-477.
MGRTVVVLGGGISGLAASYHLSRAPCPPKVVLVESSERLGGWIRSVRGPN GAIFELGPRGIRPAGALGARTLLLVSELGLDSEVLPVRGDHPAAQNRFLY VGGALHALPTGLRGLLRPSPPFSKPLFWAGLRELTKPRGKEPDETVHSFA QRRLGPEVASLAMDSLCRGVFAGNSRELSIRSCFPSLFQAEQTHRSILLG LLLGAGRTPQPDSALIRQALAERWSQWSLRGGLEMLPQALETHLTSRGVS VLRGQPVCGLSLQAEGRWKVSLRDSSLEADHVISAIPASVLSELLPAEAA PLARALSAITAVSVAVVNLQYQGAHLPVQGFGHLVPSSEDPGVLGIVYDS VAFPEQDGSPPGLRVTVMLGGSWLQTLEASGCVLSQELFQQRAQEAAATQ LGLKEMPSHCLVHLHKNCIPQYTLGHWQKLESARQFLTAHRLPLTLAGAS YEGVAVNDCIESGRQAAVSVLGTEPNS
Database link: P50336
- WB: A549 and HepG2 whole cell lysates. IHC-P: Human testis tissue. ICC/IF: PC-3 cells. IP: HepG2 whole cell lysate.
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We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%.
Our Abpromise guarantee covers the use of ab236853 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/5000. Predicted molecular weight: 51 kDa.|
|IP||1/200 - 1/2000.|
|IHC-P||1/20 - 1/200.|
|ICC/IF||1/50 - 1/200.|
FunctionCatalyzes the 6-electron oxidation of protoporphyrinogen-IX to form protoporphyrin-IX.
Tissue specificityExpressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
PathwayPorphyrin metabolism; protoporphyrin-IX biosynthesis; protoporphyrin-IX from protoporphyrinogen-IX: step 1/1.
Involvement in diseaseDefects in PPOX are the cause of variegate porphyria (VP) [MIM:176200]. Porphyrias are inherited defects in the biosynthesis of heme, resulting in the accumulation and increased excretion of porphyrins or porphyrin precursors. They are classified as erythropoietic or hepatic, depending on whether the enzyme deficiency occurs in red blood cells or in the liver. PV is the most common form of porphyria in South Africa. It is characterized by skin hyperpigmentation and hypertrichosis, abdominal pain, tachycardia, hypertension and neuromuscular disturbances. High fecal levels of protoporphyrin and coproporphyrin, increased urine uroporphyrins and iron overload are typical markers of the disease.
Sequence similaritiesBelongs to the protoporphyrinogen oxidase family.
Cellular localizationMitochondrion inner membrane.
- Information by UniProt
- MGC8485 antibody
- PPO antibody
- PPOX antibody
All lanes : Anti-PPOX antibody (ab236853) at 1/500 dilution
Lane 1 : A549 (human lung carcinoma cell line) whole cell lysate
Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 51 kDa
Paraffin-embedded human testis tissue stained for PPOX using ab236853 at 1/100 dilution in immunohistochemical analysis.
PC-3 (human prostate adenocarcinoma cell line) cells stained for PPOX (green) using ab236853 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).
PPOX was immunoprecipitated from 500 µg HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate with ab236853 at 1/200 dilution.
Lane 1: Rabbit control IgG IP in HepG2 whole cell lysate.
Lane 2: ab236853 IP in HepG2 whole cell lysate.
Lane 3: HepG2 whole cell lysate 10 µg (Input).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab236853 has not yet been referenced specifically in any publications.