Product nameAnti-PPOX antibody [EPR10400]
See all PPOX primary antibodies
DescriptionRabbit monoclonal [EPR10400] to PPOX
Tested applicationsSuitable for: WB, ICC/IF, Flow Cytmore details
Unsuitable for: IHC-P or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human PPOX aa 50-150 (Cysteine residue). The exact sequence is proprietary.
Database link: P50336
- HepG2, HT-1080, 293T and A549 cell lysates. A549 cells.Permeabilized HT-1080 cells.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at 4°C (stable for up to 12 months). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab170412 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa).|
|ICC/IF||1/100 - 1/250.|
|Flow Cyt||1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionCatalyzes the 6-electron oxidation of protoporphyrinogen-IX to form protoporphyrin-IX.
Tissue specificityExpressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
PathwayPorphyrin metabolism; protoporphyrin-IX biosynthesis; protoporphyrin-IX from protoporphyrinogen-IX: step 1/1.
Involvement in diseaseDefects in PPOX are the cause of variegate porphyria (VP) [MIM:176200]. Porphyrias are inherited defects in the biosynthesis of heme, resulting in the accumulation and increased excretion of porphyrins or porphyrin precursors. They are classified as erythropoietic or hepatic, depending on whether the enzyme deficiency occurs in red blood cells or in the liver. PV is the most common form of porphyria in South Africa. It is characterized by skin hyperpigmentation and hypertrichosis, abdominal pain, tachycardia, hypertension and neuromuscular disturbances. High fecal levels of protoporphyrin and coproporphyrin, increased urine uroporphyrins and iron overload are typical markers of the disease.
Sequence similaritiesBelongs to the protoporphyrinogen oxidase family.
Cellular localizationMitochondrion inner membrane.
- Information by UniProt
- MGC8485 antibody
- PPO antibody
- PPOX antibody
All lanes : Anti-PPOX antibody [EPR10400] (ab170412) at 1/1000 dilution
Lane 1 : A549 cell lysates
Lane 2 : HepG2 cell lysates
Lane 3 : HT-1080 cell lysates
Lane 4 : 293T cell lysates
Lysates/proteins at 10 µg per lane.
Predicted band size: 51 kDa
Immunocytochemistry/ Immunofluorescence analysis of A549 cells labeling PPOX with ab170412 at a 1/100 dilution (green). DAPI nuclear staining (blue).
Flow cytometric analysis of permeabilized HT-1080 cells using ab170412 (red) at a 1/100 dilution or a rabbit IgG (negative) (green).
ab170412 has been referenced in 1 publication.
- Vandekeere S et al. Serine Synthesis via PHGDH Is Essential for Heme Production in Endothelial Cells. Cell Metab N/A:N/A (2018). PubMed: 30017355