Overview

  • Product name
  • Description
    Rabbit polyclonal to PPP1A
  • Host species
    Rabbit
  • Specificity
    Recognises recombinant PP1 beta, PP1 gamma 1 and PP1 gamma 2.
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Cow, Human
    Predicted to work with: Rabbit, Dog
  • Immunogen

    Synthetic peptide:

    RPIT PPRNSAKAKK

    conjugated to KLH, corresponding to C terminal amino acids 317-330 of Human PPP1A

  • Positive control
    • Total rat brain homogenate.

Properties

Applications

Our Abpromise guarantee covers the use of ab16446 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 38 kDa.
IP Use at an assay dependent dilution.

Target

  • Function
    Protein phosphatase 1 (PP1) is essential for cell division, and participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. Involved in regulation of ionic conductances and long-term synaptic plasticity. May play an important role in dephosphorylating substrates such as the postsynaptic density-associated Ca(2+)/calmodulin dependent protein kinase II. Component of the PTW/PP1 phosphatase complex, which plays a role in the control of chromatin structure and cell cycle progression during the transition from mitosis into interphase.
  • Sequence similarities
    Belongs to the PPP phosphatase family. PP-1 subfamily.
  • Cellular localization
    Cytoplasm. Nucleus. Nucleus > nucleoplasm. Nucleus > nucleolus. Primarily nuclear and largely excluded from the nucleolus. Highly mobile in cells and can be relocalized through interaction with targeting subunits. NOM1 plays a role in targeting this protein to the nucleolus. In the presence of PPP1R8 relocalizes from the nucleus to nuclear speckles.
  • Information by UniProt
  • Database links
  • Alternative names
    • Alpha isoform serine threonine protein phosphatase PP1alpha 1 catalytic subunit antibody
    • Catalytic subunit antibody
    • EC 3.1.3.16 antibody
    • MGC15877 antibody
    • MGC1674 antibody
    • PP 1A antibody
    • PP-1A antibody
    • PP1A antibody
    • PP1A_HUMAN antibody
    • PP1alpha antibody
    • PP2C ALPHA antibody
    • PP2CA antibody
    • Ppp1ca antibody
    • Protein Phosphatase 2C Alpha Isoform antibody
    • Serine threonine protein phosphatase PP1 alpha catalytic subunit antibody
    • Serine threonine protein phosphatase PP1 alpha catalytic subunit protein phosphatase 1 antibody
    • Serine/threonine-protein phosphatase PP1-alpha catalytic subunit antibody
    see all

References

This product has been referenced in:
  • Freire PP  et al. Obesity does not lead to imbalance between myocardial phospholamban phosphorylation and dephosphorylation. Arq Bras Cardiol 103:41-50 (2014). Read more (PubMed: 25120084) »
See 1 Publication for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Answer

Thank you for your feedback and for your cooperation. Should the suggestions not improve the results, please do not hesitate to contact me again with the further requested details. I wish you the best of luck with your research. Have a good weekend!

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Answer

Thank you for taking the time to complete our questionnaire and contact us. I appreciate the time taken to submit further information to us and I am sorry to hear you have been experiencing problems with a vial of ab16446. We have received no complaints about this product, therefore I am concerned it is not working for you in IP, although it is fine in Western blot. Unfortunately, we currently do not have a different lot of this PPP1A antibody available. Therefore, I would like to make some suggestions to the protocol in an attempt to improve the results obtained in IP. I would also appreciate if you can confirm some details of the procedure. 1) Is 2 µg/ml the optimised antibody concentration? The amount of antibody used needs to be optimised depending on the abundance of the protein and the affinity of the antibody for the protein as well as the method used: The first approach is to mix antibody with protein sample and allowing it to interact with protein followed by addition of Protein A/G support. The second approach (which I understand you have used) is to bind antibody to the Protein A/G beads and then mix with the antigen.This method gives lesser yield than first one and may require more antibody. Please find attached our detailed protocol guide. 2) Could you please confirm which positive control you have used, in order to check the Protein A-agarose and other reagents? 3) Which elution buffer have you used and for how long have you incubated to elute the proteins from the beads? I hope optimisation of the IP conditions will improve the results. As the antibody was purchased just recently you are covered by our Abpromise guarantee. Therefore if the suggestions do not improve the results I would be happy to offer a refund or replacement vial of a primary antibody of your choice. Please do not hesitate to contact me should you have additional questions.

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Question
Answer

Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this PPP1A antibody in IP. We will happily offer technical support and in the event that a product is not functioning in the applications cited on the product data sheet (and the problem has been reported within 6 months of purchase), we will be pleased to provide a credit note, free of charge replacement or refund. I would like to investigate this particular case further for you, and also obtain more information for our quality records. In order to proceed with this, I have enclosed a questionnaire. I would appreciate if you could complete this. It will help you put the information we require together very easily. I would appreciate if you could also provide an image which would help us to assess the results. Thank you for your time and cooperation. We look forward to receiving the completed questionnaire.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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