Anti-PPP1A (phospho T320) antibody [EP1512Y] (ab62334)


  • Product name
    Anti-PPP1A (phospho T320) antibody [EP1512Y]
    See all PPP1A primary antibodies
  • Description
    Rabbit monoclonal [EP1512Y] to PPP1A (phospho T320)
  • Host species
  • Specificity
    This antibody detects PP1 alpha phosphorylated at Threonine 320.
  • Tested applications
    Suitable for: WB, IP, IHC-Pmore details
    Unsuitable for: Flow Cyt or ICC
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Xenopus laevis
  • Immunogen

    Phospho specific peptide corresponding to residues surrounding Threonine 320 of human PP1A.

  • Positive control
    • HeLa cell lysate and human muscle tissue.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents



Our Abpromise guarantee covers the use of ab62334 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa).
IP 1/40.
IHC-P 1/100 - 1/250. An antigen retrieval step is strongly recommended.
  • Application notes
    Is unsuitable for Flow Cyt or ICC.
  • Target

    • Function
      Protein phosphatase 1 (PP1) is essential for cell division, and participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. Involved in regulation of ionic conductances and long-term synaptic plasticity. May play an important role in dephosphorylating substrates such as the postsynaptic density-associated Ca(2+)/calmodulin dependent protein kinase II. Component of the PTW/PP1 phosphatase complex, which plays a role in the control of chromatin structure and cell cycle progression during the transition from mitosis into interphase.
    • Sequence similarities
      Belongs to the PPP phosphatase family. PP-1 subfamily.
    • Cellular localization
      Cytoplasm. Nucleus. Nucleus > nucleoplasm. Nucleus > nucleolus. Primarily nuclear and largely excluded from the nucleolus. Highly mobile in cells and can be relocalized through interaction with targeting subunits. NOM1 plays a role in targeting this protein to the nucleolus. In the presence of PPP1R8 relocalizes from the nucleus to nuclear speckles.
    • Information by UniProt
    • Database links
    • Alternative names
      • Alpha isoform serine threonine protein phosphatase PP1alpha 1 catalytic subunit antibody
      • Catalytic subunit antibody
      • EC antibody
      • MGC15877 antibody
      • MGC1674 antibody
      • PP 1A antibody
      • PP-1A antibody
      • PP1A antibody
      • PP1A_HUMAN antibody
      • PP1alpha antibody
      • PP2C ALPHA antibody
      • PP2CA antibody
      • Ppp1ca antibody
      • Protein Phosphatase 2C Alpha Isoform antibody
      • Serine threonine protein phosphatase PP1 alpha catalytic subunit antibody
      • Serine threonine protein phosphatase PP1 alpha catalytic subunit protein phosphatase 1 antibody
      • Serine/threonine-protein phosphatase PP1-alpha catalytic subunit antibody
      see all


    • All lanes : Anti-PPP1A (phospho T320) antibody [EP1512Y] (ab62334)

      Lane 1 : HeLa cell lysate (untreated)
      Lane 2 : HeLa cell lysate (treated with Calyculin A)

      Lysates/proteins at 10 µg per lane.

      All lanes : Goat anti-rabbit HRP conjugate at 1/2000 dilution

      Predicted band size: 38 kDa
      Observed band size: 38 kDa

      The bottom image shows beta Tubulin as a positive control.
    • Ab62334 (1/100-1/250) staining human PP1A in human muscle tissue by immunohistochemistry using paraffin embedded tissue.


    This product has been referenced in:
    • Chassé H  et al. Cyclin B Translation Depends on mTOR Activity after Fertilization in Sea Urchin Embryos. PLoS One 11:e0150318 (2016). Read more (PubMed: 26962866) »
    • Horiuchi D  et al. MYC pathway activation in triple-negative breast cancer is synthetic lethal with CDK inhibition. J Exp Med 209:679-96 (2012). Read more (PubMed: 22430491) »

    See all 4 Publications for this product

    Customer reviews and Q&As

    I am very pleased to hear you would like to test ab62334 in rabbit. This code will give you: 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview forrabbitand include this code in the “Additional Com...

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