Overview

  • Product name

    Anti-PPP1A/PPP1CA antibody [1C11-C10-H5-E9]
    See all PPP1A/PPP1CA primary antibodies
  • Description

    Mouse monoclonal [1C11-C10-H5-E9] to PPP1A/PPP1CA
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Rabbit, Human, Pig
  • Immunogen

    Recombinant fragment corresponding to Human PPP1A/PPP1CA. Expressed in E.coli
    Database link: NM_001008709

  • Positive control

    • A431, HeLa and Jurkat Whole cell lysates, Human breast cancer tissue, HeLa cells
  • General notes

    This product was changed from ascites to tissue culture supernatant on 17.08.2018.  Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

     This product was previously labelled as PPP1A

     

Properties

Applications

Our Abpromise guarantee covers the use of ab150782 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 37 kDa.
IHC-P 1/500 - 1/1000.
ICC/IF 1/300.

Target

  • Function

    Protein phosphatase 1 (PP1) is essential for cell division, and participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. Involved in regulation of ionic conductances and long-term synaptic plasticity. May play an important role in dephosphorylating substrates such as the postsynaptic density-associated Ca(2+)/calmodulin dependent protein kinase II. Component of the PTW/PP1 phosphatase complex, which plays a role in the control of chromatin structure and cell cycle progression during the transition from mitosis into interphase.
  • Sequence similarities

    Belongs to the PPP phosphatase family. PP-1 subfamily.
  • Cellular localization

    Cytoplasm. Nucleus. Nucleus > nucleoplasm. Nucleus > nucleolus. Primarily nuclear and largely excluded from the nucleolus. Highly mobile in cells and can be relocalized through interaction with targeting subunits. NOM1 plays a role in targeting this protein to the nucleolus. In the presence of PPP1R8 relocalizes from the nucleus to nuclear speckles.
  • Information by UniProt
  • Database links

  • Alternative names

    • Alpha isoform serine threonine protein phosphatase PP1alpha 1 catalytic subunit antibody
    • Catalytic subunit antibody
    • EC 3.1.3.16 antibody
    • MGC15877 antibody
    • MGC1674 antibody
    • PP 1A antibody
    • PP-1A antibody
    • PP1A antibody
    • PP1A_HUMAN antibody
    • PP1alpha antibody
    • PP2C ALPHA antibody
    • PP2CA antibody
    • Ppp1ca antibody
    • Protein Phosphatase 2C Alpha Isoform antibody
    • Serine threonine protein phosphatase PP1 alpha catalytic subunit antibody
    • Serine threonine protein phosphatase PP1 alpha catalytic subunit protein phosphatase 1 antibody
    • Serine/threonine-protein phosphatase PP1-alpha catalytic subunit antibody
    see all

Images

  • All lanes : Anti-PPP1A/PPP1CA antibody [1C11-C10-H5-E9] (ab150782) at 1/1000 dilution

    Lane 1 : K562 cell lysates
    Lane 2 : 3T3 cell lysates
    Lane 3 : Hela cell lysates

    Predicted band size: 37 kDa

  • All lanes : Anti-PPP1A/PPP1CA antibody [1C11-C10-H5-E9] (ab150782) at 1/1000 dilution

    Lane 1 : A431 whole cell lysates
    Lane 2 : Jurkat whole cell lysates
    Lane 3 : HeLa whole cell lysates

    Predicted band size: 37 kDa



    This image was produced with antibody produced in ascites. 

  • Immunohistochemistry analysis of paraffin-embedded Human breast cancer tissue, labelling PPP1A/PPP1CA using ab150782 at a 1/500 dilution. This image was produced with antibody produced in ascites. 

  • Immunocytochemistry analysis of HeLa cells, labelling PPP1A/PPP1CA using ab150782 at a 1/300 dilution. This image was produced with antibody produced in ascites. 

References

ab150782 has not yet been referenced specifically in any publications.

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